Experiment: R2A with A22 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS26165 and RR42_RS26170 are separated by 469 nucleotides RR42_RS26170 and RR42_RS26175 are separated by 108 nucleotides
RR42_RS26165: RR42_RS26165 - DNA-binding protein, at 1,226,689 to 1,228,125
_RS26165
RR42_RS26170: RR42_RS26170 - rod shape-determining protein MreB, at 1,228,595 to 1,229,638
_RS26170
RR42_RS26175: RR42_RS26175 - gamma-glutamyltransferase, at 1,229,747 to 1,231,612
_RS26175
Position (kb)
1228
1229
1230 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1227.610 kb on + strand, within RR42_RS26165 at 1227.610 kb on + strand, within RR42_RS26165 at 1227.610 kb on + strand, within RR42_RS26165 at 1227.610 kb on + strand, within RR42_RS26165 at 1227.611 kb on - strand, within RR42_RS26165 at 1227.611 kb on - strand, within RR42_RS26165 at 1227.611 kb on - strand, within RR42_RS26165 at 1227.611 kb on - strand, within RR42_RS26165 at 1227.611 kb on - strand, within RR42_RS26165 at 1227.611 kb on - strand, within RR42_RS26165 at 1227.611 kb on - strand, within RR42_RS26165 at 1227.658 kb on + strand, within RR42_RS26165 at 1227.658 kb on + strand, within RR42_RS26165 at 1227.658 kb on + strand, within RR42_RS26165 at 1227.658 kb on + strand, within RR42_RS26165 at 1227.658 kb on + strand, within RR42_RS26165 at 1227.659 kb on - strand, within RR42_RS26165 at 1227.776 kb on - strand, within RR42_RS26165 at 1227.776 kb on - strand, within RR42_RS26165 at 1227.776 kb on - strand, within RR42_RS26165 at 1227.808 kb on + strand, within RR42_RS26165 at 1227.808 kb on + strand, within RR42_RS26165 at 1227.850 kb on + strand, within RR42_RS26165 at 1227.850 kb on + strand, within RR42_RS26165 at 1227.850 kb on + strand, within RR42_RS26165 at 1227.851 kb on - strand, within RR42_RS26165 at 1228.076 kb on + strand at 1228.077 kb on - strand at 1228.220 kb on + strand at 1228.270 kb on + strand at 1228.270 kb on + strand at 1228.270 kb on + strand at 1228.271 kb on - strand at 1228.271 kb on - strand at 1228.271 kb on - strand at 1228.351 kb on + strand at 1228.351 kb on + strand at 1228.351 kb on + strand at 1228.351 kb on + strand at 1228.352 kb on - strand at 1228.352 kb on - strand at 1228.437 kb on + strand at 1228.438 kb on - strand at 1228.438 kb on - strand at 1228.518 kb on + strand at 1228.519 kb on - strand at 1228.519 kb on - strand at 1228.519 kb on - strand at 1228.524 kb on + strand at 1228.524 kb on + strand at 1228.613 kb on - strand at 1228.617 kb on - strand at 1228.644 kb on + strand at 1228.645 kb on - strand at 1228.645 kb on - strand at 1228.667 kb on + strand at 1228.667 kb on + strand at 1228.668 kb on - strand at 1228.794 kb on + strand, within RR42_RS26170 at 1229.012 kb on + strand, within RR42_RS26170 at 1229.012 kb on + strand, within RR42_RS26170 at 1229.144 kb on + strand, within RR42_RS26170 at 1229.145 kb on - strand, within RR42_RS26170 at 1229.145 kb on - strand, within RR42_RS26170 at 1229.145 kb on - strand, within RR42_RS26170 at 1229.198 kb on + strand, within RR42_RS26170 at 1229.198 kb on + strand, within RR42_RS26170 at 1229.199 kb on - strand, within RR42_RS26170 at 1229.199 kb on - strand, within RR42_RS26170 at 1229.213 kb on + strand, within RR42_RS26170 at 1229.213 kb on + strand, within RR42_RS26170 at 1229.630 kb on + strand at 1230.027 kb on + strand, within RR42_RS26175 at 1230.028 kb on - strand, within RR42_RS26175 at 1230.028 kb on - strand, within RR42_RS26175 at 1230.243 kb on + strand, within RR42_RS26175 at 1230.244 kb on - strand, within RR42_RS26175 at 1230.426 kb on + strand, within RR42_RS26175 at 1230.483 kb on + strand, within RR42_RS26175 at 1230.483 kb on + strand, within RR42_RS26175 at 1230.484 kb on - strand, within RR42_RS26175 at 1230.484 kb on - strand, within RR42_RS26175 at 1230.501 kb on + strand, within RR42_RS26175 at 1230.604 kb on - strand, within RR42_RS26175
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.01 mg/ml remove 1,227,610 + RR42_RS26165 0.64 -1.7 1,227,610 + RR42_RS26165 0.64 -0.5 1,227,610 + RR42_RS26165 0.64 -1.3 1,227,610 + RR42_RS26165 0.64 -0.1 1,227,611 - RR42_RS26165 0.64 -0.4 1,227,611 - RR42_RS26165 0.64 +0.7 1,227,611 - RR42_RS26165 0.64 -0.5 1,227,611 - RR42_RS26165 0.64 -0.2 1,227,611 - RR42_RS26165 0.64 +1.4 1,227,611 - RR42_RS26165 0.64 +0.7 1,227,611 - RR42_RS26165 0.64 +1.4 1,227,658 + RR42_RS26165 0.67 +0.1 1,227,658 + RR42_RS26165 0.67 -0.8 1,227,658 + RR42_RS26165 0.67 +0.0 1,227,658 + RR42_RS26165 0.67 +0.4 1,227,658 + RR42_RS26165 0.67 +1.0 1,227,659 - RR42_RS26165 0.68 -0.7 1,227,776 - RR42_RS26165 0.76 -1.0 1,227,776 - RR42_RS26165 0.76 +0.4 1,227,776 - RR42_RS26165 0.76 +0.7 1,227,808 + RR42_RS26165 0.78 -1.5 1,227,808 + RR42_RS26165 0.78 -0.6 1,227,850 + RR42_RS26165 0.81 -1.1 1,227,850 + RR42_RS26165 0.81 -1.1 1,227,850 + RR42_RS26165 0.81 -1.0 1,227,851 - RR42_RS26165 0.81 +0.8 1,228,076 + +0.8 1,228,077 - -0.3 1,228,220 + -3.6 1,228,270 + -0.4 1,228,270 + +0.8 1,228,270 + -1.8 1,228,271 - +1.2 1,228,271 - -0.1 1,228,271 - +0.8 1,228,351 + -0.9 1,228,351 + +0.1 1,228,351 + -1.1 1,228,351 + -0.2 1,228,352 - +0.5 1,228,352 - +0.5 1,228,437 + -0.9 1,228,438 - +0.0 1,228,438 - -0.3 1,228,518 + -0.2 1,228,519 - +1.4 1,228,519 - +0.6 1,228,519 - +1.0 1,228,524 + -0.0 1,228,524 + +0.3 1,228,613 - +1.1 1,228,617 - +0.5 1,228,644 + +0.1 1,228,645 - +1.2 1,228,645 - +0.9 1,228,667 + -2.2 1,228,667 + +0.9 1,228,668 - +1.0 1,228,794 + RR42_RS26170 0.19 +0.5 1,229,012 + RR42_RS26170 0.40 +0.5 1,229,012 + RR42_RS26170 0.40 -0.6 1,229,144 + RR42_RS26170 0.53 +0.5 1,229,145 - RR42_RS26170 0.53 +1.1 1,229,145 - RR42_RS26170 0.53 +0.8 1,229,145 - RR42_RS26170 0.53 -0.2 1,229,198 + RR42_RS26170 0.58 +1.1 1,229,198 + RR42_RS26170 0.58 +1.2 1,229,199 - RR42_RS26170 0.58 +1.4 1,229,199 - RR42_RS26170 0.58 -1.8 1,229,213 + RR42_RS26170 0.59 +0.3 1,229,213 + RR42_RS26170 0.59 +1.6 1,229,630 + +0.5 1,230,027 + RR42_RS26175 0.15 +0.4 1,230,028 - RR42_RS26175 0.15 -0.8 1,230,028 - RR42_RS26175 0.15 +0.1 1,230,243 + RR42_RS26175 0.27 +0.3 1,230,244 - RR42_RS26175 0.27 +0.3 1,230,426 + RR42_RS26175 0.36 -0.5 1,230,483 + RR42_RS26175 0.39 -0.4 1,230,483 + RR42_RS26175 0.39 -0.1 1,230,484 - RR42_RS26175 0.39 +0.1 1,230,484 - RR42_RS26175 0.39 -1.6 1,230,501 + RR42_RS26175 0.40 -0.1 1,230,604 - RR42_RS26175 0.46 -0.7
Or see this region's nucleotide sequence