Experiment: R2A with A22 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS25995 and RR42_RS26000 are separated by 200 nucleotides RR42_RS26000 and RR42_RS26005 are separated by 330 nucleotides
RR42_RS25995: RR42_RS25995 - cytochrome o ubiquinol oxidase subunit I, at 1,175,125 to 1,177,101
_RS25995
RR42_RS26000: RR42_RS26000 - cytochrome O ubiquinol oxidase subunit II, at 1,177,302 to 1,178,063
_RS26000
RR42_RS26005: RR42_RS26005 - GntR family transcriptional regulator, at 1,178,394 to 1,179,797
_RS26005
Position (kb)
1177
1178
1179 Strain fitness (log2 ratio)
-2
-1
0
1 at 1176.309 kb on + strand, within RR42_RS25995 at 1176.514 kb on + strand, within RR42_RS25995 at 1176.529 kb on + strand, within RR42_RS25995 at 1176.529 kb on + strand, within RR42_RS25995 at 1176.529 kb on + strand, within RR42_RS25995 at 1176.529 kb on + strand, within RR42_RS25995 at 1176.529 kb on + strand, within RR42_RS25995 at 1176.529 kb on + strand, within RR42_RS25995 at 1176.530 kb on - strand, within RR42_RS25995 at 1176.530 kb on - strand, within RR42_RS25995 at 1176.530 kb on - strand, within RR42_RS25995 at 1176.533 kb on + strand, within RR42_RS25995 at 1176.534 kb on - strand, within RR42_RS25995 at 1176.534 kb on - strand, within RR42_RS25995 at 1176.534 kb on - strand, within RR42_RS25995 at 1176.534 kb on - strand, within RR42_RS25995 at 1176.534 kb on - strand, within RR42_RS25995 at 1176.561 kb on - strand, within RR42_RS25995 at 1176.806 kb on + strand, within RR42_RS25995 at 1176.806 kb on + strand, within RR42_RS25995 at 1176.824 kb on + strand, within RR42_RS25995 at 1176.974 kb on + strand at 1176.992 kb on + strand at 1177.218 kb on + strand at 1177.218 kb on + strand at 1177.236 kb on - strand at 1177.293 kb on - strand at 1177.307 kb on - strand at 1177.307 kb on - strand at 1177.307 kb on - strand at 1177.307 kb on - strand at 1177.513 kb on + strand, within RR42_RS26000 at 1177.534 kb on + strand, within RR42_RS26000 at 1177.534 kb on + strand, within RR42_RS26000 at 1177.535 kb on - strand, within RR42_RS26000 at 1177.535 kb on - strand, within RR42_RS26000 at 1177.591 kb on + strand, within RR42_RS26000 at 1177.591 kb on + strand, within RR42_RS26000 at 1177.709 kb on - strand, within RR42_RS26000 at 1177.717 kb on + strand, within RR42_RS26000 at 1177.793 kb on - strand, within RR42_RS26000 at 1177.909 kb on + strand, within RR42_RS26000 at 1177.909 kb on + strand, within RR42_RS26000 at 1177.909 kb on + strand, within RR42_RS26000 at 1177.909 kb on + strand, within RR42_RS26000 at 1177.910 kb on - strand, within RR42_RS26000 at 1178.052 kb on + strand at 1178.053 kb on - strand at 1178.195 kb on + strand at 1178.201 kb on + strand at 1178.202 kb on - strand at 1178.213 kb on + strand at 1178.214 kb on - strand at 1178.217 kb on + strand at 1178.218 kb on - strand at 1178.218 kb on - strand at 1178.228 kb on + strand at 1178.317 kb on - strand at 1178.362 kb on + strand at 1178.363 kb on - strand at 1178.367 kb on + strand at 1178.414 kb on - strand at 1178.809 kb on - strand, within RR42_RS26005 at 1178.809 kb on - strand, within RR42_RS26005 at 1178.809 kb on - strand, within RR42_RS26005
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.01 mg/ml remove 1,176,309 + RR42_RS25995 0.60 +0.3 1,176,514 + RR42_RS25995 0.70 +0.2 1,176,529 + RR42_RS25995 0.71 +1.1 1,176,529 + RR42_RS25995 0.71 +0.2 1,176,529 + RR42_RS25995 0.71 +0.4 1,176,529 + RR42_RS25995 0.71 -0.6 1,176,529 + RR42_RS25995 0.71 -0.4 1,176,529 + RR42_RS25995 0.71 -0.0 1,176,530 - RR42_RS25995 0.71 -0.1 1,176,530 - RR42_RS25995 0.71 -0.7 1,176,530 - RR42_RS25995 0.71 +0.3 1,176,533 + RR42_RS25995 0.71 -2.5 1,176,534 - RR42_RS25995 0.71 -1.2 1,176,534 - RR42_RS25995 0.71 +0.7 1,176,534 - RR42_RS25995 0.71 -0.3 1,176,534 - RR42_RS25995 0.71 -0.5 1,176,534 - RR42_RS25995 0.71 -0.6 1,176,561 - RR42_RS25995 0.73 +0.3 1,176,806 + RR42_RS25995 0.85 -0.1 1,176,806 + RR42_RS25995 0.85 +0.6 1,176,824 + RR42_RS25995 0.86 -0.2 1,176,974 + +0.2 1,176,992 + -0.9 1,177,218 + -0.1 1,177,218 + -0.6 1,177,236 - -0.3 1,177,293 - -0.2 1,177,307 - -0.5 1,177,307 - +0.6 1,177,307 - +1.2 1,177,307 - -0.8 1,177,513 + RR42_RS26000 0.28 -1.0 1,177,534 + RR42_RS26000 0.30 -1.4 1,177,534 + RR42_RS26000 0.30 -0.9 1,177,535 - RR42_RS26000 0.31 +0.1 1,177,535 - RR42_RS26000 0.31 -0.7 1,177,591 + RR42_RS26000 0.38 -1.2 1,177,591 + RR42_RS26000 0.38 +1.1 1,177,709 - RR42_RS26000 0.53 +0.2 1,177,717 + RR42_RS26000 0.54 -0.1 1,177,793 - RR42_RS26000 0.64 +0.5 1,177,909 + RR42_RS26000 0.80 -0.6 1,177,909 + RR42_RS26000 0.80 +0.8 1,177,909 + RR42_RS26000 0.80 +1.3 1,177,909 + RR42_RS26000 0.80 -2.2 1,177,910 - RR42_RS26000 0.80 +0.8 1,178,052 + +0.0 1,178,053 - +0.3 1,178,195 + -0.8 1,178,201 + +1.0 1,178,202 - +0.8 1,178,213 + -0.4 1,178,214 - +1.7 1,178,217 + -0.6 1,178,218 - -0.2 1,178,218 - +0.0 1,178,228 + +0.8 1,178,317 - -0.1 1,178,362 + -0.4 1,178,363 - -0.2 1,178,367 + +0.2 1,178,414 - +0.3 1,178,809 - RR42_RS26005 0.30 +0.4 1,178,809 - RR42_RS26005 0.30 -0.7 1,178,809 - RR42_RS26005 0.30 +0.2
Or see this region's nucleotide sequence