Experiment: R2A with A22 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS25465 and RR42_RS25470 are separated by 32 nucleotides RR42_RS25470 and RR42_RS25475 overlap by 4 nucleotides RR42_RS25475 and RR42_RS25480 are separated by 11 nucleotides
RR42_RS25465: RR42_RS25465 - long-chain fatty acid--CoA ligase, at 1,059,637 to 1,061,289
_RS25465
RR42_RS25470: RR42_RS25470 - alpha/beta hydrolase, at 1,061,322 to 1,062,113
_RS25470
RR42_RS25475: RR42_RS25475 - peptidase S15, at 1,062,110 to 1,063,024
_RS25475
RR42_RS25480: RR42_RS25480 - enoyl-CoA hydratase, at 1,063,036 to 1,063,845
_RS25480
Position (kb)
1061
1062
1063 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1060.518 kb on + strand, within RR42_RS25465 at 1060.518 kb on + strand, within RR42_RS25465 at 1060.519 kb on - strand, within RR42_RS25465 at 1060.519 kb on - strand, within RR42_RS25465 at 1060.519 kb on - strand, within RR42_RS25465 at 1060.533 kb on - strand, within RR42_RS25465 at 1060.566 kb on - strand, within RR42_RS25465 at 1060.613 kb on + strand, within RR42_RS25465 at 1060.680 kb on + strand, within RR42_RS25465 at 1060.681 kb on - strand, within RR42_RS25465 at 1060.707 kb on - strand, within RR42_RS25465 at 1060.743 kb on + strand, within RR42_RS25465 at 1060.745 kb on + strand, within RR42_RS25465 at 1060.746 kb on - strand, within RR42_RS25465 at 1060.824 kb on - strand, within RR42_RS25465 at 1060.965 kb on + strand, within RR42_RS25465 at 1060.966 kb on - strand, within RR42_RS25465 at 1060.966 kb on - strand, within RR42_RS25465 at 1060.966 kb on - strand, within RR42_RS25465 at 1060.967 kb on + strand, within RR42_RS25465 at 1060.968 kb on - strand, within RR42_RS25465 at 1061.012 kb on - strand, within RR42_RS25465 at 1061.051 kb on + strand, within RR42_RS25465 at 1061.085 kb on + strand, within RR42_RS25465 at 1061.157 kb on - strand at 1061.160 kb on + strand at 1061.160 kb on + strand at 1061.161 kb on - strand at 1061.161 kb on - strand at 1061.172 kb on + strand at 1061.172 kb on + strand at 1061.247 kb on - strand at 1061.247 kb on - strand at 1061.655 kb on + strand, within RR42_RS25470 at 1061.844 kb on - strand, within RR42_RS25470 at 1062.042 kb on + strand at 1062.047 kb on - strand at 1062.103 kb on - strand at 1062.113 kb on + strand at 1062.113 kb on + strand at 1062.145 kb on - strand at 1062.351 kb on + strand, within RR42_RS25475 at 1062.351 kb on + strand, within RR42_RS25475 at 1062.351 kb on + strand, within RR42_RS25475 at 1062.351 kb on + strand, within RR42_RS25475 at 1062.352 kb on - strand, within RR42_RS25475 at 1062.457 kb on - strand, within RR42_RS25475 at 1062.632 kb on + strand, within RR42_RS25475 at 1062.684 kb on + strand, within RR42_RS25475 at 1062.685 kb on - strand, within RR42_RS25475 at 1062.742 kb on - strand, within RR42_RS25475 at 1062.894 kb on + strand, within RR42_RS25475 at 1062.894 kb on + strand, within RR42_RS25475 at 1063.005 kb on + strand at 1063.005 kb on + strand at 1063.005 kb on + strand at 1063.006 kb on - strand at 1063.006 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.01 mg/ml remove 1,060,518 + RR42_RS25465 0.53 -0.1 1,060,518 + RR42_RS25465 0.53 -0.1 1,060,519 - RR42_RS25465 0.53 +0.3 1,060,519 - RR42_RS25465 0.53 -0.9 1,060,519 - RR42_RS25465 0.53 -0.8 1,060,533 - RR42_RS25465 0.54 -0.2 1,060,566 - RR42_RS25465 0.56 +1.8 1,060,613 + RR42_RS25465 0.59 +0.0 1,060,680 + RR42_RS25465 0.63 +1.9 1,060,681 - RR42_RS25465 0.63 +0.9 1,060,707 - RR42_RS25465 0.65 +0.5 1,060,743 + RR42_RS25465 0.67 -0.5 1,060,745 + RR42_RS25465 0.67 -0.7 1,060,746 - RR42_RS25465 0.67 -0.5 1,060,824 - RR42_RS25465 0.72 +0.9 1,060,965 + RR42_RS25465 0.80 +0.5 1,060,966 - RR42_RS25465 0.80 +0.3 1,060,966 - RR42_RS25465 0.80 +1.4 1,060,966 - RR42_RS25465 0.80 +0.5 1,060,967 + RR42_RS25465 0.80 +1.1 1,060,968 - RR42_RS25465 0.81 -0.9 1,061,012 - RR42_RS25465 0.83 +0.0 1,061,051 + RR42_RS25465 0.86 -0.3 1,061,085 + RR42_RS25465 0.88 -0.2 1,061,157 - +0.5 1,061,160 + +1.8 1,061,160 + -0.7 1,061,161 - -1.4 1,061,161 - -0.3 1,061,172 + +1.4 1,061,172 + -2.3 1,061,247 - +0.1 1,061,247 - -0.2 1,061,655 + RR42_RS25470 0.42 -1.3 1,061,844 - RR42_RS25470 0.66 -1.2 1,062,042 + -0.9 1,062,047 - +0.3 1,062,103 - -0.9 1,062,113 + +0.4 1,062,113 + -0.6 1,062,145 - +0.5 1,062,351 + RR42_RS25475 0.26 +0.4 1,062,351 + RR42_RS25475 0.26 -2.2 1,062,351 + RR42_RS25475 0.26 -0.1 1,062,351 + RR42_RS25475 0.26 +0.2 1,062,352 - RR42_RS25475 0.26 +0.5 1,062,457 - RR42_RS25475 0.38 +1.1 1,062,632 + RR42_RS25475 0.57 -0.1 1,062,684 + RR42_RS25475 0.63 +0.9 1,062,685 - RR42_RS25475 0.63 -0.1 1,062,742 - RR42_RS25475 0.69 +1.9 1,062,894 + RR42_RS25475 0.86 -0.4 1,062,894 + RR42_RS25475 0.86 +0.1 1,063,005 + +0.5 1,063,005 + +0.1 1,063,005 + +0.6 1,063,006 - +0.2 1,063,006 - -0.3
Or see this region's nucleotide sequence