Experiment: R2A with A22 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS25200 and RR42_RS25205 are separated by 375 nucleotides RR42_RS25205 and RR42_RS25210 are separated by 172 nucleotides
RR42_RS25200: RR42_RS25200 - TetR family transcriptional regulator, at 995,493 to 996,071
_RS25200
RR42_RS25205: RR42_RS25205 - glucose 1-dehydrogenase, at 996,447 to 997,181
_RS25205
RR42_RS25210: RR42_RS25210 - acetyl-CoA acetyltransferase, at 997,354 to 998,538
_RS25210
Position (kb) 996
997
998 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 995.453 kb on + strand at 995.453 kb on + strand at 995.454 kb on - strand at 995.546 kb on + strand at 995.546 kb on + strand at 995.634 kb on + strand, within RR42_RS25200 at 995.634 kb on + strand, within RR42_RS25200 at 995.634 kb on + strand, within RR42_RS25200 at 995.635 kb on - strand, within RR42_RS25200 at 995.635 kb on - strand, within RR42_RS25200 at 995.636 kb on + strand, within RR42_RS25200 at 995.763 kb on + strand, within RR42_RS25200 at 995.764 kb on - strand, within RR42_RS25200 at 996.084 kb on + strand at 996.084 kb on + strand at 996.084 kb on + strand at 996.084 kb on + strand at 996.085 kb on - strand at 996.085 kb on - strand at 996.145 kb on + strand at 996.299 kb on + strand at 996.355 kb on - strand at 996.355 kb on - strand at 996.355 kb on - strand at 996.355 kb on - strand at 996.618 kb on + strand, within RR42_RS25205 at 996.619 kb on - strand, within RR42_RS25205 at 996.619 kb on - strand, within RR42_RS25205 at 996.709 kb on + strand, within RR42_RS25205 at 996.872 kb on + strand, within RR42_RS25205 at 996.900 kb on + strand, within RR42_RS25205 at 996.901 kb on - strand, within RR42_RS25205 at 996.901 kb on - strand, within RR42_RS25205 at 996.992 kb on + strand, within RR42_RS25205 at 996.992 kb on + strand, within RR42_RS25205 at 996.992 kb on + strand, within RR42_RS25205 at 996.992 kb on + strand, within RR42_RS25205 at 996.993 kb on - strand, within RR42_RS25205 at 996.993 kb on - strand, within RR42_RS25205 at 996.993 kb on - strand, within RR42_RS25205 at 997.026 kb on - strand, within RR42_RS25205 at 997.050 kb on + strand, within RR42_RS25205 at 997.050 kb on + strand, within RR42_RS25205 at 997.091 kb on + strand, within RR42_RS25205 at 997.105 kb on - strand, within RR42_RS25205 at 997.105 kb on - strand, within RR42_RS25205 at 997.111 kb on - strand at 997.179 kb on + strand at 997.205 kb on + strand at 997.206 kb on - strand at 997.322 kb on + strand at 997.323 kb on - strand at 997.408 kb on - strand at 997.519 kb on - strand, within RR42_RS25210 at 997.519 kb on - strand, within RR42_RS25210 at 997.688 kb on + strand, within RR42_RS25210 at 997.792 kb on + strand, within RR42_RS25210 at 997.793 kb on - strand, within RR42_RS25210 at 997.793 kb on - strand, within RR42_RS25210 at 997.793 kb on - strand, within RR42_RS25210 at 997.835 kb on - strand, within RR42_RS25210 at 997.860 kb on + strand, within RR42_RS25210 at 997.861 kb on - strand, within RR42_RS25210 at 997.861 kb on - strand, within RR42_RS25210 at 997.864 kb on + strand, within RR42_RS25210 at 997.864 kb on + strand, within RR42_RS25210 at 997.865 kb on - strand, within RR42_RS25210 at 997.888 kb on - strand, within RR42_RS25210 at 997.928 kb on + strand, within RR42_RS25210 at 997.928 kb on + strand, within RR42_RS25210 at 997.929 kb on - strand, within RR42_RS25210 at 997.929 kb on - strand, within RR42_RS25210 at 997.929 kb on - strand, within RR42_RS25210 at 997.931 kb on + strand, within RR42_RS25210 at 997.932 kb on - strand, within RR42_RS25210 at 997.932 kb on - strand, within RR42_RS25210 at 997.932 kb on - strand, within RR42_RS25210 at 997.998 kb on - strand, within RR42_RS25210
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.01 mg/ml remove 995,453 + -0.4 995,453 + +0.1 995,454 - -1.8 995,546 + -1.3 995,546 + -0.6 995,634 + RR42_RS25200 0.24 +0.5 995,634 + RR42_RS25200 0.24 +0.4 995,634 + RR42_RS25200 0.24 -0.6 995,635 - RR42_RS25200 0.25 +0.9 995,635 - RR42_RS25200 0.25 -0.9 995,636 + RR42_RS25200 0.25 -0.6 995,763 + RR42_RS25200 0.47 +1.9 995,764 - RR42_RS25200 0.47 +0.3 996,084 + -0.0 996,084 + -0.2 996,084 + -0.7 996,084 + +0.3 996,085 - +0.8 996,085 - -0.9 996,145 + +0.4 996,299 + +0.6 996,355 - -0.7 996,355 - +0.2 996,355 - +0.4 996,355 - +1.5 996,618 + RR42_RS25205 0.23 +0.0 996,619 - RR42_RS25205 0.23 -3.1 996,619 - RR42_RS25205 0.23 +0.7 996,709 + RR42_RS25205 0.36 -1.3 996,872 + RR42_RS25205 0.58 -0.4 996,900 + RR42_RS25205 0.62 -0.3 996,901 - RR42_RS25205 0.62 -2.1 996,901 - RR42_RS25205 0.62 -0.4 996,992 + RR42_RS25205 0.74 -0.3 996,992 + RR42_RS25205 0.74 -0.1 996,992 + RR42_RS25205 0.74 -0.6 996,992 + RR42_RS25205 0.74 -1.4 996,993 - RR42_RS25205 0.74 +0.2 996,993 - RR42_RS25205 0.74 +0.1 996,993 - RR42_RS25205 0.74 -0.3 997,026 - RR42_RS25205 0.79 +0.0 997,050 + RR42_RS25205 0.82 -0.1 997,050 + RR42_RS25205 0.82 -0.8 997,091 + RR42_RS25205 0.88 +1.1 997,105 - RR42_RS25205 0.90 -0.2 997,105 - RR42_RS25205 0.90 +0.2 997,111 - +3.4 997,179 + -0.3 997,205 + -0.2 997,206 - -0.1 997,322 + -0.3 997,323 - -0.2 997,408 - +0.1 997,519 - RR42_RS25210 0.14 -0.5 997,519 - RR42_RS25210 0.14 -2.7 997,688 + RR42_RS25210 0.28 +0.3 997,792 + RR42_RS25210 0.37 +0.3 997,793 - RR42_RS25210 0.37 -0.7 997,793 - RR42_RS25210 0.37 -0.7 997,793 - RR42_RS25210 0.37 -0.8 997,835 - RR42_RS25210 0.41 +0.7 997,860 + RR42_RS25210 0.43 +0.7 997,861 - RR42_RS25210 0.43 +0.7 997,861 - RR42_RS25210 0.43 -0.6 997,864 + RR42_RS25210 0.43 +0.9 997,864 + RR42_RS25210 0.43 -0.4 997,865 - RR42_RS25210 0.43 -0.5 997,888 - RR42_RS25210 0.45 +1.8 997,928 + RR42_RS25210 0.48 +0.5 997,928 + RR42_RS25210 0.48 +0.6 997,929 - RR42_RS25210 0.49 -0.3 997,929 - RR42_RS25210 0.49 -0.7 997,929 - RR42_RS25210 0.49 +0.2 997,931 + RR42_RS25210 0.49 -0.3 997,932 - RR42_RS25210 0.49 -0.5 997,932 - RR42_RS25210 0.49 +0.2 997,932 - RR42_RS25210 0.49 -0.7 997,998 - RR42_RS25210 0.54 -0.4
Or see this region's nucleotide sequence