Experiment: R2A with A22 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS25010 and RR42_RS25015 are separated by 150 nucleotides RR42_RS25015 and RR42_RS25020 are separated by 22 nucleotides RR42_RS25020 and RR42_RS25025 are separated by 21 nucleotides
RR42_RS25010: RR42_RS25010 - betaine-aldehyde dehydrogenase, at 948,856 to 950,313
_RS25010
RR42_RS25015: RR42_RS25015 - gluconolactonase, at 950,464 to 951,348
_RS25015
RR42_RS25020: RR42_RS25020 - amino acid ABC transporter ATPase, at 951,371 to 952,129
_RS25020
RR42_RS25025: RR42_RS25025 - sugar ABC transporter ATPase, at 952,151 to 952,921
_RS25025
Position (kb) 950
951
952 Strain fitness (log2 ratio) -1
0
1 at 949.881 kb on + strand, within RR42_RS25010 at 949.881 kb on + strand, within RR42_RS25010 at 949.881 kb on + strand, within RR42_RS25010 at 949.882 kb on - strand, within RR42_RS25010 at 949.946 kb on + strand, within RR42_RS25010 at 949.987 kb on - strand, within RR42_RS25010 at 950.067 kb on + strand, within RR42_RS25010 at 950.249 kb on - strand at 950.397 kb on - strand at 950.397 kb on - strand at 950.397 kb on - strand at 950.600 kb on + strand, within RR42_RS25015 at 950.600 kb on + strand, within RR42_RS25015 at 950.601 kb on - strand, within RR42_RS25015 at 951.263 kb on + strand at 951.269 kb on + strand at 951.269 kb on + strand at 951.269 kb on + strand at 951.270 kb on - strand at 951.270 kb on - strand at 951.396 kb on + strand at 951.397 kb on - strand at 952.164 kb on + strand at 952.165 kb on - strand at 952.242 kb on + strand, within RR42_RS25025 at 952.243 kb on - strand, within RR42_RS25025 at 952.243 kb on - strand, within RR42_RS25025 at 952.243 kb on - strand, within RR42_RS25025 at 952.248 kb on + strand, within RR42_RS25025 at 952.249 kb on - strand, within RR42_RS25025
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.01 mg/ml remove 949,881 + RR42_RS25010 0.70 +0.5 949,881 + RR42_RS25010 0.70 +0.3 949,881 + RR42_RS25010 0.70 -0.4 949,882 - RR42_RS25010 0.70 +0.3 949,946 + RR42_RS25010 0.75 -0.2 949,987 - RR42_RS25010 0.78 +0.8 950,067 + RR42_RS25010 0.83 -1.1 950,249 - +0.6 950,397 - -1.1 950,397 - -0.5 950,397 - -0.6 950,600 + RR42_RS25015 0.15 -1.0 950,600 + RR42_RS25015 0.15 -0.8 950,601 - RR42_RS25015 0.15 -0.7 951,263 + -0.7 951,269 + +0.4 951,269 + +0.5 951,269 + -0.2 951,270 - -0.2 951,270 - +0.5 951,396 + -0.8 951,397 - -1.3 952,164 + -0.3 952,165 - -0.1 952,242 + RR42_RS25025 0.12 +1.6 952,243 - RR42_RS25025 0.12 +0.3 952,243 - RR42_RS25025 0.12 +0.2 952,243 - RR42_RS25025 0.12 -0.2 952,248 + RR42_RS25025 0.13 +0.7 952,249 - RR42_RS25025 0.13 +0.1
Or see this region's nucleotide sequence