Experiment: R2A with A22 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS24975 and RR42_RS24980 are separated by 23 nucleotides RR42_RS24980 and RR42_RS24985 overlap by 2 nucleotides RR42_RS24985 and RR42_RS24990 overlap by 4 nucleotides
RR42_RS24975: RR42_RS24975 - acetyl-CoA hydrolase, at 941,301 to 942,617
_RS24975
RR42_RS24980: RR42_RS24980 - 3-alpha,7-alpha, 12-alpha-trihydroxy-5-beta-cholest-24-enoyl-CoA hydratase, at 942,641 to 943,534
_RS24980
RR42_RS24985: RR42_RS24985 - hypothetical protein, at 943,533 to 943,787
_RS24985
RR42_RS24990: RR42_RS24990 - acyl-CoA dehydrogenase, at 943,784 to 944,950
_RS24990
Position (kb) 942
943
944 Strain fitness (log2 ratio) -2
-1
0
1
2 at 941.742 kb on - strand, within RR42_RS24975 at 941.743 kb on + strand, within RR42_RS24975 at 941.743 kb on + strand, within RR42_RS24975 at 941.743 kb on + strand, within RR42_RS24975 at 941.743 kb on + strand, within RR42_RS24975 at 941.743 kb on + strand, within RR42_RS24975 at 941.743 kb on + strand, within RR42_RS24975 at 941.744 kb on - strand, within RR42_RS24975 at 942.133 kb on + strand, within RR42_RS24975 at 942.133 kb on + strand, within RR42_RS24975 at 942.133 kb on + strand, within RR42_RS24975 at 942.134 kb on - strand, within RR42_RS24975 at 942.202 kb on + strand, within RR42_RS24975 at 942.203 kb on - strand, within RR42_RS24975 at 942.226 kb on + strand, within RR42_RS24975 at 942.264 kb on + strand, within RR42_RS24975 at 942.265 kb on - strand, within RR42_RS24975 at 942.294 kb on + strand, within RR42_RS24975 at 942.328 kb on - strand, within RR42_RS24975 at 943.081 kb on - strand, within RR42_RS24980 at 943.452 kb on + strand at 943.453 kb on - strand at 943.453 kb on - strand at 943.476 kb on + strand at 943.476 kb on + strand at 943.476 kb on + strand at 943.476 kb on + strand at 943.521 kb on + strand at 943.522 kb on - strand at 943.546 kb on + strand at 943.546 kb on + strand at 943.546 kb on + strand at 943.600 kb on - strand, within RR42_RS24985 at 943.600 kb on - strand, within RR42_RS24985 at 943.648 kb on + strand, within RR42_RS24985 at 943.833 kb on + strand at 943.866 kb on - strand at 943.922 kb on - strand, within RR42_RS24990 at 943.961 kb on + strand, within RR42_RS24990 at 944.057 kb on + strand, within RR42_RS24990 at 944.057 kb on + strand, within RR42_RS24990 at 944.058 kb on - strand, within RR42_RS24990 at 944.058 kb on - strand, within RR42_RS24990 at 944.058 kb on - strand, within RR42_RS24990 at 944.225 kb on + strand, within RR42_RS24990 at 944.226 kb on - strand, within RR42_RS24990 at 944.226 kb on - strand, within RR42_RS24990 at 944.495 kb on + strand, within RR42_RS24990 at 944.496 kb on - strand, within RR42_RS24990 at 944.496 kb on - strand, within RR42_RS24990 at 944.496 kb on - strand, within RR42_RS24990
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.01 mg/ml remove 941,742 - RR42_RS24975 0.33 -0.3 941,743 + RR42_RS24975 0.34 +0.9 941,743 + RR42_RS24975 0.34 +0.3 941,743 + RR42_RS24975 0.34 +0.5 941,743 + RR42_RS24975 0.34 +0.9 941,743 + RR42_RS24975 0.34 -0.0 941,743 + RR42_RS24975 0.34 -0.8 941,744 - RR42_RS24975 0.34 +0.4 942,133 + RR42_RS24975 0.63 -0.2 942,133 + RR42_RS24975 0.63 +0.7 942,133 + RR42_RS24975 0.63 +0.3 942,134 - RR42_RS24975 0.63 -0.8 942,202 + RR42_RS24975 0.68 -0.1 942,203 - RR42_RS24975 0.68 +1.7 942,226 + RR42_RS24975 0.70 -1.0 942,264 + RR42_RS24975 0.73 -0.1 942,265 - RR42_RS24975 0.73 +1.8 942,294 + RR42_RS24975 0.75 -0.1 942,328 - RR42_RS24975 0.78 +0.7 943,081 - RR42_RS24980 0.49 -0.9 943,452 + +0.7 943,453 - -2.1 943,453 - -0.5 943,476 + -1.1 943,476 + +0.3 943,476 + -0.2 943,476 + -1.5 943,521 + +0.1 943,522 - +0.4 943,546 + +0.8 943,546 + -0.9 943,546 + +0.1 943,600 - RR42_RS24985 0.26 -0.9 943,600 - RR42_RS24985 0.26 +0.1 943,648 + RR42_RS24985 0.45 +0.9 943,833 + +1.2 943,866 - -0.9 943,922 - RR42_RS24990 0.12 +0.3 943,961 + RR42_RS24990 0.15 +0.3 944,057 + RR42_RS24990 0.23 +0.4 944,057 + RR42_RS24990 0.23 +0.0 944,058 - RR42_RS24990 0.23 -0.2 944,058 - RR42_RS24990 0.23 +1.5 944,058 - RR42_RS24990 0.23 +0.2 944,225 + RR42_RS24990 0.38 +0.0 944,226 - RR42_RS24990 0.38 +0.2 944,226 - RR42_RS24990 0.38 +0.5 944,495 + RR42_RS24990 0.61 +0.6 944,496 - RR42_RS24990 0.61 -0.9 944,496 - RR42_RS24990 0.61 +0.3 944,496 - RR42_RS24990 0.61 -0.3
Or see this region's nucleotide sequence