Experiment: R2A with A22 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS24510 and RR42_RS24515 overlap by 4 nucleotides RR42_RS24515 and RR42_RS24520 overlap by 4 nucleotides RR42_RS24520 and RR42_RS24525 are separated by 938 nucleotides
RR42_RS24510: RR42_RS24510 - hypothetical protein, at 831,928 to 832,356
_RS24510
RR42_RS24515: RR42_RS24515 - hypothetical protein, at 832,353 to 833,003
_RS24515
RR42_RS24520: RR42_RS24520 - hypothetical protein, at 833,000 to 833,551
_RS24520
RR42_RS24525: RR42_RS24525 - hypothetical protein, at 834,490 to 835,209
_RS24525
Position (kb) 832
833
834 Strain fitness (log2 ratio) -1
0
1
2 at 832.212 kb on + strand, within RR42_RS24510 at 832.213 kb on - strand, within RR42_RS24510 at 832.670 kb on + strand, within RR42_RS24515 at 832.671 kb on - strand, within RR42_RS24515 at 832.756 kb on + strand, within RR42_RS24515 at 832.756 kb on + strand, within RR42_RS24515 at 832.793 kb on - strand, within RR42_RS24515 at 833.035 kb on - strand at 833.035 kb on - strand at 833.035 kb on - strand at 833.052 kb on + strand at 833.053 kb on - strand at 833.053 kb on - strand at 833.053 kb on - strand at 833.053 kb on - strand at 833.194 kb on - strand, within RR42_RS24520 at 833.194 kb on - strand, within RR42_RS24520 at 833.280 kb on + strand, within RR42_RS24520 at 833.281 kb on - strand, within RR42_RS24520 at 833.569 kb on - strand at 833.635 kb on + strand at 833.635 kb on + strand at 833.651 kb on + strand at 833.958 kb on + strand at 833.958 kb on + strand at 833.958 kb on + strand at 833.959 kb on - strand at 833.981 kb on + strand at 834.129 kb on - strand at 834.129 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.01 mg/ml remove 832,212 + RR42_RS24510 0.66 -0.2 832,213 - RR42_RS24510 0.66 +0.7 832,670 + RR42_RS24515 0.49 +0.2 832,671 - RR42_RS24515 0.49 +0.3 832,756 + RR42_RS24515 0.62 +0.3 832,756 + RR42_RS24515 0.62 +0.4 832,793 - RR42_RS24515 0.68 +0.4 833,035 - +0.6 833,035 - +1.0 833,035 - +1.1 833,052 + -0.2 833,053 - +1.7 833,053 - +2.3 833,053 - +1.0 833,053 - -0.0 833,194 - RR42_RS24520 0.35 +0.7 833,194 - RR42_RS24520 0.35 +0.1 833,280 + RR42_RS24520 0.51 +0.8 833,281 - RR42_RS24520 0.51 +1.2 833,569 - +0.1 833,635 + +0.8 833,635 + +1.0 833,651 + +0.1 833,958 + -0.5 833,958 + +1.0 833,958 + +0.3 833,959 - -0.0 833,981 + -0.3 834,129 - -0.0 834,129 - -0.2
Or see this region's nucleotide sequence