Experiment: R2A with A22 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS24175 and RR42_RS24180 are separated by 29 nucleotides
RR42_RS24175: RR42_RS24175 - isopropylmalate isomerase, at 749,287 to 750,696
_RS24175
RR42_RS24180: RR42_RS24180 - 3-isopropylmalate dehydratase, at 750,726 to 751,376
_RS24180
Position (kb) 750
751
752 Strain fitness (log2 ratio) -2
-1
0
1 at 750.010 kb on + strand, within RR42_RS24175 at 750.044 kb on + strand, within RR42_RS24175 at 750.045 kb on - strand, within RR42_RS24175 at 750.259 kb on + strand, within RR42_RS24175 at 750.259 kb on + strand, within RR42_RS24175 at 750.259 kb on + strand, within RR42_RS24175 at 750.260 kb on - strand, within RR42_RS24175 at 750.260 kb on - strand, within RR42_RS24175 at 750.260 kb on - strand, within RR42_RS24175 at 750.340 kb on - strand, within RR42_RS24175 at 750.340 kb on - strand, within RR42_RS24175 at 750.586 kb on - strand at 750.586 kb on - strand at 750.659 kb on + strand at 750.659 kb on + strand at 750.660 kb on - strand at 750.660 kb on - strand at 750.702 kb on + strand at 750.703 kb on - strand at 750.706 kb on + strand at 750.706 kb on + strand at 750.707 kb on - strand at 750.742 kb on + strand at 750.742 kb on + strand at 750.742 kb on + strand at 750.742 kb on + strand at 750.931 kb on + strand, within RR42_RS24180 at 751.087 kb on - strand, within RR42_RS24180 at 751.167 kb on + strand, within RR42_RS24180 at 751.167 kb on + strand, within RR42_RS24180 at 751.168 kb on - strand, within RR42_RS24180 at 751.225 kb on - strand, within RR42_RS24180 at 751.254 kb on + strand, within RR42_RS24180 at 751.255 kb on - strand, within RR42_RS24180 at 751.379 kb on + strand at 751.379 kb on + strand at 751.379 kb on + strand at 751.379 kb on + strand at 751.379 kb on + strand at 751.380 kb on - strand at 751.457 kb on + strand at 751.458 kb on - strand at 751.472 kb on + strand at 751.499 kb on + strand at 751.521 kb on - strand at 751.557 kb on + strand at 751.558 kb on - strand at 751.601 kb on + strand at 751.617 kb on + strand at 751.618 kb on - strand at 751.630 kb on + strand at 751.630 kb on + strand at 751.669 kb on + strand at 751.669 kb on + strand at 751.692 kb on + strand at 751.692 kb on + strand at 751.734 kb on - strand at 751.770 kb on + strand at 751.770 kb on + strand at 751.770 kb on + strand at 751.770 kb on + strand at 751.771 kb on - strand at 751.771 kb on - strand at 751.953 kb on + strand at 751.953 kb on + strand at 751.954 kb on - strand at 751.988 kb on - strand at 751.988 kb on - strand at 752.019 kb on + strand at 752.019 kb on + strand at 752.020 kb on - strand at 752.020 kb on - strand at 752.150 kb on - strand at 752.179 kb on - strand at 752.216 kb on + strand at 752.217 kb on - strand at 752.228 kb on + strand at 752.282 kb on + strand at 752.282 kb on + strand at 752.311 kb on + strand at 752.311 kb on + strand at 752.312 kb on - strand at 752.359 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.01 mg/ml remove 750,010 + RR42_RS24175 0.51 -1.1 750,044 + RR42_RS24175 0.54 +1.3 750,045 - RR42_RS24175 0.54 +0.2 750,259 + RR42_RS24175 0.69 -0.9 750,259 + RR42_RS24175 0.69 +0.3 750,259 + RR42_RS24175 0.69 -2.6 750,260 - RR42_RS24175 0.69 -0.2 750,260 - RR42_RS24175 0.69 -0.0 750,260 - RR42_RS24175 0.69 +0.0 750,340 - RR42_RS24175 0.75 +1.6 750,340 - RR42_RS24175 0.75 -1.4 750,586 - -0.4 750,586 - -0.2 750,659 + -0.2 750,659 + +0.7 750,660 - -0.0 750,660 - -0.5 750,702 + -0.1 750,703 - +0.2 750,706 + +1.2 750,706 + +1.2 750,707 - -0.0 750,742 + -0.1 750,742 + +0.3 750,742 + +0.2 750,742 + -0.5 750,931 + RR42_RS24180 0.31 +0.2 751,087 - RR42_RS24180 0.55 +1.1 751,167 + RR42_RS24180 0.68 -0.6 751,167 + RR42_RS24180 0.68 -0.5 751,168 - RR42_RS24180 0.68 +0.6 751,225 - RR42_RS24180 0.77 +1.0 751,254 + RR42_RS24180 0.81 +0.8 751,255 - RR42_RS24180 0.81 +1.0 751,379 + -0.3 751,379 + +0.2 751,379 + -0.1 751,379 + +0.4 751,379 + -0.7 751,380 - -0.3 751,457 + +0.0 751,458 - -0.1 751,472 + +0.2 751,499 + +1.5 751,521 - +1.2 751,557 + +0.3 751,558 - -0.4 751,601 + +0.8 751,617 + -0.7 751,618 - -0.1 751,630 + -1.3 751,630 + -0.5 751,669 + -0.0 751,669 + +0.3 751,692 + +0.7 751,692 + +1.3 751,734 - -0.3 751,770 + -0.6 751,770 + +1.0 751,770 + -0.4 751,770 + -0.0 751,771 - +0.7 751,771 - +0.6 751,953 + +1.4 751,953 + +0.0 751,954 - +0.1 751,988 - +0.0 751,988 - +0.3 752,019 + -1.3 752,019 + +0.2 752,020 - +0.5 752,020 - +0.4 752,150 - +0.8 752,179 - -1.4 752,216 + +0.2 752,217 - -0.3 752,228 + +0.6 752,282 + -0.3 752,282 + -0.4 752,311 + +0.9 752,311 + -0.3 752,312 - -0.5 752,359 - +1.3
Or see this region's nucleotide sequence