Experiment: R2A with A22 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS23735 and RR42_RS23740 are separated by 6 nucleotides RR42_RS23740 and RR42_RS23745 are separated by 26 nucleotides RR42_RS23745 and RR42_RS23750 are separated by 66 nucleotides
RR42_RS23735: RR42_RS23735 - phenylacetyl-CoA 1,2-epoxidase, structural subunit (EC 1.14.13.149) <small>(from data)</small>, at 649,875 to 650,675
_RS23735
RR42_RS23740: RR42_RS23740 - phenylacetate-CoA oxygenase, at 650,682 to 651,248
_RS23740
RR42_RS23745: RR42_RS23745 - phenylacetic acid degradation protein, at 651,275 to 652,363
_RS23745
RR42_RS23750: RR42_RS23750 - aldehyde dehydrogenase, at 652,430 to 654,112
_RS23750
Position (kb) 651
652
653 Strain fitness (log2 ratio) -1
0
1 at 650.410 kb on - strand, within RR42_RS23735 at 650.620 kb on - strand at 650.673 kb on + strand at 650.856 kb on + strand, within RR42_RS23740 at 651.198 kb on + strand at 651.199 kb on - strand at 651.199 kb on - strand at 651.231 kb on + strand at 651.244 kb on - strand at 651.636 kb on - strand, within RR42_RS23745 at 652.136 kb on + strand, within RR42_RS23745 at 652.137 kb on - strand, within RR42_RS23745 at 652.190 kb on + strand, within RR42_RS23745 at 652.190 kb on + strand, within RR42_RS23745 at 652.191 kb on - strand, within RR42_RS23745 at 652.191 kb on - strand, within RR42_RS23745 at 652.349 kb on + strand at 652.502 kb on + strand at 652.899 kb on + strand, within RR42_RS23750 at 652.899 kb on + strand, within RR42_RS23750 at 652.913 kb on + strand, within RR42_RS23750 at 652.914 kb on - strand, within RR42_RS23750 at 652.952 kb on + strand, within RR42_RS23750 at 653.070 kb on - strand, within RR42_RS23750 at 653.348 kb on + strand, within RR42_RS23750 at 653.349 kb on - strand, within RR42_RS23750 at 653.349 kb on - strand, within RR42_RS23750 at 653.349 kb on - strand, within RR42_RS23750
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.01 mg/ml remove 650,410 - RR42_RS23735 0.67 +0.6 650,620 - +1.0 650,673 + -0.9 650,856 + RR42_RS23740 0.31 -1.0 651,198 + +0.0 651,199 - +0.1 651,199 - -0.7 651,231 + -0.2 651,244 - +1.2 651,636 - RR42_RS23745 0.33 +1.0 652,136 + RR42_RS23745 0.79 -1.0 652,137 - RR42_RS23745 0.79 -0.2 652,190 + RR42_RS23745 0.84 -1.6 652,190 + RR42_RS23745 0.84 -0.5 652,191 - RR42_RS23745 0.84 -0.4 652,191 - RR42_RS23745 0.84 -0.2 652,349 + +1.2 652,502 + +0.2 652,899 + RR42_RS23750 0.28 +1.3 652,899 + RR42_RS23750 0.28 -0.5 652,913 + RR42_RS23750 0.29 -1.7 652,914 - RR42_RS23750 0.29 +0.3 652,952 + RR42_RS23750 0.31 +0.8 653,070 - RR42_RS23750 0.38 +0.1 653,348 + RR42_RS23750 0.55 +0.3 653,349 - RR42_RS23750 0.55 -0.2 653,349 - RR42_RS23750 0.55 +0.4 653,349 - RR42_RS23750 0.55 +0.3
Or see this region's nucleotide sequence