Experiment: R2A with A22 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS23570 and RR42_RS23575 are separated by 510 nucleotides RR42_RS23575 and RR42_RS23580 are separated by 516 nucleotides
RR42_RS23570: RR42_RS23570 - flagellar M-ring protein FliF, at 613,351 to 615,060
_RS23570
RR42_RS23575: RR42_RS23575 - flagellar hook-basal body protein FliE, at 615,571 to 615,891
_RS23575
RR42_RS23580: RR42_RS23580 - hypothetical protein, at 616,408 to 617,061
_RS23580
Position (kb) 615
616 Strain fitness (log2 ratio) -2
-1
0
1
2 at 614.676 kb on - strand, within RR42_RS23570 at 614.676 kb on - strand, within RR42_RS23570 at 614.840 kb on + strand, within RR42_RS23570 at 614.906 kb on + strand at 614.906 kb on + strand at 614.907 kb on - strand at 614.907 kb on - strand at 614.918 kb on + strand at 614.919 kb on - strand at 614.919 kb on - strand at 615.182 kb on + strand at 615.182 kb on + strand at 615.183 kb on - strand at 615.210 kb on + strand at 615.210 kb on + strand at 615.223 kb on - strand at 615.262 kb on - strand at 615.329 kb on - strand at 615.380 kb on + strand at 615.421 kb on + strand at 615.492 kb on + strand at 615.492 kb on + strand at 615.493 kb on - strand at 615.576 kb on + strand at 615.576 kb on + strand at 615.576 kb on + strand at 615.577 kb on - strand at 615.577 kb on - strand at 615.810 kb on + strand, within RR42_RS23575 at 615.811 kb on - strand, within RR42_RS23575 at 615.811 kb on - strand, within RR42_RS23575 at 615.879 kb on + strand at 615.880 kb on - strand at 615.880 kb on - strand at 615.880 kb on - strand at 615.880 kb on - strand at 615.880 kb on - strand at 616.255 kb on + strand at 616.255 kb on + strand at 616.256 kb on - strand at 616.410 kb on - strand at 616.410 kb on - strand at 616.422 kb on - strand at 616.482 kb on - strand, within RR42_RS23580 at 616.586 kb on + strand, within RR42_RS23580 at 616.636 kb on + strand, within RR42_RS23580 at 616.664 kb on + strand, within RR42_RS23580 at 616.664 kb on + strand, within RR42_RS23580 at 616.665 kb on - strand, within RR42_RS23580 at 616.674 kb on - strand, within RR42_RS23580 at 616.742 kb on + strand, within RR42_RS23580 at 616.742 kb on + strand, within RR42_RS23580 at 616.838 kb on + strand, within RR42_RS23580 at 616.838 kb on + strand, within RR42_RS23580 at 616.838 kb on + strand, within RR42_RS23580 at 616.839 kb on - strand, within RR42_RS23580 at 616.839 kb on - strand, within RR42_RS23580 at 616.841 kb on + strand, within RR42_RS23580 at 616.841 kb on + strand, within RR42_RS23580 at 616.841 kb on + strand, within RR42_RS23580 at 616.847 kb on + strand, within RR42_RS23580 at 616.847 kb on + strand, within RR42_RS23580 at 616.848 kb on - strand, within RR42_RS23580 at 616.848 kb on - strand, within RR42_RS23580 at 616.848 kb on - strand, within RR42_RS23580 at 616.848 kb on - strand, within RR42_RS23580 at 616.856 kb on + strand, within RR42_RS23580
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.01 mg/ml remove 614,676 - RR42_RS23570 0.77 +0.1 614,676 - RR42_RS23570 0.77 +1.7 614,840 + RR42_RS23570 0.87 +0.3 614,906 + -0.9 614,906 + -0.2 614,907 - +0.1 614,907 - -0.7 614,918 + -0.1 614,919 - -0.3 614,919 - -0.9 615,182 + -0.1 615,182 + +0.6 615,183 - -0.1 615,210 + +0.4 615,210 + -1.4 615,223 - -0.2 615,262 - -0.1 615,329 - -0.4 615,380 + -0.3 615,421 + +0.3 615,492 + -0.1 615,492 + -0.9 615,493 - -0.1 615,576 + +0.4 615,576 + +1.4 615,576 + +1.1 615,577 - -1.1 615,577 - +0.9 615,810 + RR42_RS23575 0.74 +0.6 615,811 - RR42_RS23575 0.75 +0.2 615,811 - RR42_RS23575 0.75 +0.7 615,879 + +1.8 615,880 - -0.7 615,880 - +1.1 615,880 - -0.4 615,880 - -0.4 615,880 - +0.1 616,255 + -0.3 616,255 + -0.0 616,256 - -0.3 616,410 - +0.2 616,410 - -0.4 616,422 - +1.1 616,482 - RR42_RS23580 0.11 +0.3 616,586 + RR42_RS23580 0.27 +0.3 616,636 + RR42_RS23580 0.35 -0.7 616,664 + RR42_RS23580 0.39 +2.1 616,664 + RR42_RS23580 0.39 +0.1 616,665 - RR42_RS23580 0.39 +0.2 616,674 - RR42_RS23580 0.41 +0.2 616,742 + RR42_RS23580 0.51 -0.7 616,742 + RR42_RS23580 0.51 +0.2 616,838 + RR42_RS23580 0.66 -0.8 616,838 + RR42_RS23580 0.66 -0.2 616,838 + RR42_RS23580 0.66 -0.1 616,839 - RR42_RS23580 0.66 -1.5 616,839 - RR42_RS23580 0.66 +0.2 616,841 + RR42_RS23580 0.66 -0.1 616,841 + RR42_RS23580 0.66 +0.4 616,841 + RR42_RS23580 0.66 +0.4 616,847 + RR42_RS23580 0.67 -0.0 616,847 + RR42_RS23580 0.67 +1.4 616,848 - RR42_RS23580 0.67 +0.4 616,848 - RR42_RS23580 0.67 +0.3 616,848 - RR42_RS23580 0.67 -2.1 616,848 - RR42_RS23580 0.67 -0.7 616,856 + RR42_RS23580 0.69 -0.2
Or see this region's nucleotide sequence