Experiment: R2A with A22 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS23555 and RR42_RS23560 overlap by 8 nucleotides RR42_RS23560 and RR42_RS23565 overlap by 14 nucleotides RR42_RS23565 and RR42_RS23570 overlap by 11 nucleotides
RR42_RS23555: RR42_RS23555 - ATP synthase, at 610,069 to 611,532
_RS23555
RR42_RS23560: RR42_RS23560 - flagellar assembly protein FliH, at 611,525 to 612,382
_RS23560
RR42_RS23565: RR42_RS23565 - flagellar motor switch protein G, at 612,369 to 613,361
_RS23565
RR42_RS23570: RR42_RS23570 - flagellar M-ring protein FliF, at 613,351 to 615,060
_RS23570
Position (kb) 612
613
614 Strain fitness (log2 ratio) -2
-1
0
1
2 at 611.523 kb on + strand at 611.524 kb on - strand at 611.524 kb on - strand at 611.524 kb on - strand at 611.524 kb on - strand at 611.789 kb on + strand, within RR42_RS23560 at 611.979 kb on + strand, within RR42_RS23560 at 611.979 kb on + strand, within RR42_RS23560 at 611.980 kb on - strand, within RR42_RS23560 at 612.028 kb on - strand, within RR42_RS23560 at 612.218 kb on - strand, within RR42_RS23560 at 612.376 kb on + strand at 612.376 kb on + strand at 612.376 kb on + strand at 612.377 kb on - strand at 612.377 kb on - strand at 612.399 kb on + strand at 612.430 kb on - strand at 612.430 kb on - strand at 612.733 kb on - strand, within RR42_RS23565 at 613.291 kb on - strand at 613.389 kb on + strand at 613.389 kb on + strand at 613.390 kb on - strand at 613.390 kb on - strand at 613.559 kb on + strand, within RR42_RS23570 at 613.560 kb on - strand, within RR42_RS23570 at 613.583 kb on - strand, within RR42_RS23570 at 613.625 kb on + strand, within RR42_RS23570 at 613.853 kb on + strand, within RR42_RS23570 at 613.853 kb on + strand, within RR42_RS23570 at 613.853 kb on + strand, within RR42_RS23570 at 613.921 kb on - strand, within RR42_RS23570 at 613.934 kb on + strand, within RR42_RS23570 at 613.935 kb on - strand, within RR42_RS23570 at 614.226 kb on - strand, within RR42_RS23570 at 614.226 kb on - strand, within RR42_RS23570 at 614.226 kb on - strand, within RR42_RS23570 at 614.336 kb on + strand, within RR42_RS23570 at 614.336 kb on + strand, within RR42_RS23570 at 614.337 kb on - strand, within RR42_RS23570
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.01 mg/ml remove 611,523 + +0.5 611,524 - -1.3 611,524 - +1.1 611,524 - +0.4 611,524 - -0.9 611,789 + RR42_RS23560 0.31 -0.0 611,979 + RR42_RS23560 0.53 -0.2 611,979 + RR42_RS23560 0.53 -0.0 611,980 - RR42_RS23560 0.53 +0.6 612,028 - RR42_RS23560 0.59 -1.9 612,218 - RR42_RS23560 0.81 -0.8 612,376 + -0.3 612,376 + -1.0 612,376 + -0.1 612,377 - +0.7 612,377 - +0.4 612,399 + -0.9 612,430 - -0.7 612,430 - +0.5 612,733 - RR42_RS23565 0.37 -1.7 613,291 - +0.1 613,389 + -0.1 613,389 + +0.4 613,390 - -0.0 613,390 - +0.1 613,559 + RR42_RS23570 0.12 -0.3 613,560 - RR42_RS23570 0.12 -0.7 613,583 - RR42_RS23570 0.14 -0.1 613,625 + RR42_RS23570 0.16 -1.4 613,853 + RR42_RS23570 0.29 +1.3 613,853 + RR42_RS23570 0.29 -0.6 613,853 + RR42_RS23570 0.29 +0.9 613,921 - RR42_RS23570 0.33 +0.3 613,934 + RR42_RS23570 0.34 +0.5 613,935 - RR42_RS23570 0.34 +1.3 614,226 - RR42_RS23570 0.51 -0.0 614,226 - RR42_RS23570 0.51 +0.1 614,226 - RR42_RS23570 0.51 +2.0 614,336 + RR42_RS23570 0.58 -0.9 614,336 + RR42_RS23570 0.58 -0.1 614,337 - RR42_RS23570 0.58 -0.4
Or see this region's nucleotide sequence