Experiment: R2A with A22 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS23395 and RR42_RS23400 are separated by 213 nucleotides RR42_RS23400 and RR42_RS23405 are separated by 264 nucleotides
RR42_RS23395: RR42_RS23395 - LysR family transcriptional regulator, at 576,264 to 577,151
_RS23395
RR42_RS23400: RR42_RS23400 - ABC transporter substrate-binding protein, at 577,365 to 578,354
_RS23400
RR42_RS23405: RR42_RS23405 - hypothetical protein, at 578,619 to 579,512
_RS23405
Position (kb)
577
578
579 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 576.376 kb on - strand, within RR42_RS23395 at 576.442 kb on - strand, within RR42_RS23395 at 576.488 kb on + strand, within RR42_RS23395 at 576.702 kb on - strand, within RR42_RS23395 at 576.702 kb on - strand, within RR42_RS23395 at 576.789 kb on - strand, within RR42_RS23395 at 576.789 kb on - strand, within RR42_RS23395 at 576.789 kb on - strand, within RR42_RS23395 at 576.816 kb on - strand, within RR42_RS23395 at 576.845 kb on - strand, within RR42_RS23395 at 576.849 kb on - strand, within RR42_RS23395 at 576.879 kb on - strand, within RR42_RS23395 at 577.073 kb on + strand at 577.074 kb on - strand at 577.178 kb on - strand at 577.184 kb on - strand at 577.227 kb on + strand at 577.228 kb on - strand at 577.245 kb on + strand at 577.247 kb on + strand at 577.248 kb on - strand at 577.248 kb on - strand at 577.326 kb on - strand at 577.341 kb on - strand at 577.341 kb on - strand at 577.351 kb on - strand at 577.373 kb on - strand at 577.494 kb on + strand, within RR42_RS23400 at 577.568 kb on + strand, within RR42_RS23400 at 577.569 kb on - strand, within RR42_RS23400 at 577.569 kb on - strand, within RR42_RS23400 at 577.645 kb on - strand, within RR42_RS23400 at 577.645 kb on - strand, within RR42_RS23400 at 577.646 kb on + strand, within RR42_RS23400 at 577.646 kb on + strand, within RR42_RS23400 at 577.646 kb on + strand, within RR42_RS23400 at 577.647 kb on - strand, within RR42_RS23400 at 577.647 kb on - strand, within RR42_RS23400 at 577.647 kb on - strand, within RR42_RS23400 at 577.677 kb on + strand, within RR42_RS23400 at 577.707 kb on + strand, within RR42_RS23400 at 577.708 kb on - strand, within RR42_RS23400 at 577.725 kb on + strand, within RR42_RS23400 at 577.725 kb on + strand, within RR42_RS23400 at 577.726 kb on - strand, within RR42_RS23400 at 577.776 kb on - strand, within RR42_RS23400 at 577.839 kb on + strand, within RR42_RS23400 at 577.839 kb on + strand, within RR42_RS23400 at 577.840 kb on - strand, within RR42_RS23400 at 577.932 kb on + strand, within RR42_RS23400 at 577.933 kb on - strand, within RR42_RS23400 at 577.979 kb on - strand, within RR42_RS23400 at 577.979 kb on - strand, within RR42_RS23400 at 577.979 kb on - strand, within RR42_RS23400 at 578.008 kb on + strand, within RR42_RS23400 at 578.009 kb on - strand, within RR42_RS23400 at 578.010 kb on + strand, within RR42_RS23400 at 578.010 kb on + strand, within RR42_RS23400 at 578.011 kb on - strand, within RR42_RS23400 at 578.011 kb on - strand, within RR42_RS23400 at 578.157 kb on - strand, within RR42_RS23400 at 578.244 kb on - strand, within RR42_RS23400 at 578.613 kb on + strand at 578.614 kb on - strand at 578.661 kb on + strand at 578.750 kb on - strand, within RR42_RS23405 at 578.837 kb on + strand, within RR42_RS23405 at 578.838 kb on - strand, within RR42_RS23405 at 578.876 kb on - strand, within RR42_RS23405 at 578.924 kb on + strand, within RR42_RS23405 at 579.201 kb on + strand, within RR42_RS23405 at 579.202 kb on - strand, within RR42_RS23405 at 579.327 kb on + strand, within RR42_RS23405 at 579.327 kb on + strand, within RR42_RS23405 at 579.328 kb on - strand, within RR42_RS23405 at 579.328 kb on - strand, within RR42_RS23405
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.01 mg/ml remove 576,376 - RR42_RS23395 0.13 -0.7 576,442 - RR42_RS23395 0.20 -2.7 576,488 + RR42_RS23395 0.25 -1.2 576,702 - RR42_RS23395 0.49 +0.9 576,702 - RR42_RS23395 0.49 +0.3 576,789 - RR42_RS23395 0.59 -0.1 576,789 - RR42_RS23395 0.59 +1.1 576,789 - RR42_RS23395 0.59 -0.8 576,816 - RR42_RS23395 0.62 +0.3 576,845 - RR42_RS23395 0.65 -1.6 576,849 - RR42_RS23395 0.66 +0.7 576,879 - RR42_RS23395 0.69 -0.3 577,073 + +0.1 577,074 - -0.2 577,178 - -0.9 577,184 - +0.5 577,227 + -0.7 577,228 - -0.2 577,245 + +0.7 577,247 + -0.6 577,248 - +0.2 577,248 - +0.3 577,326 - -0.9 577,341 - +1.1 577,341 - +1.2 577,351 - -0.1 577,373 - +0.9 577,494 + RR42_RS23400 0.13 +2.1 577,568 + RR42_RS23400 0.21 +0.3 577,569 - RR42_RS23400 0.21 -0.1 577,569 - RR42_RS23400 0.21 -0.1 577,645 - RR42_RS23400 0.28 +1.3 577,645 - RR42_RS23400 0.28 -0.9 577,646 + RR42_RS23400 0.28 +0.1 577,646 + RR42_RS23400 0.28 -1.0 577,646 + RR42_RS23400 0.28 -0.2 577,647 - RR42_RS23400 0.28 -0.0 577,647 - RR42_RS23400 0.28 +0.4 577,647 - RR42_RS23400 0.28 +0.1 577,677 + RR42_RS23400 0.32 -0.3 577,707 + RR42_RS23400 0.35 +1.3 577,708 - RR42_RS23400 0.35 -0.5 577,725 + RR42_RS23400 0.36 -0.4 577,725 + RR42_RS23400 0.36 -0.9 577,726 - RR42_RS23400 0.36 +0.9 577,776 - RR42_RS23400 0.42 -0.9 577,839 + RR42_RS23400 0.48 -1.2 577,839 + RR42_RS23400 0.48 +0.4 577,840 - RR42_RS23400 0.48 -0.0 577,932 + RR42_RS23400 0.57 +0.3 577,933 - RR42_RS23400 0.57 -0.5 577,979 - RR42_RS23400 0.62 +0.8 577,979 - RR42_RS23400 0.62 +0.5 577,979 - RR42_RS23400 0.62 -0.0 578,008 + RR42_RS23400 0.65 -0.2 578,009 - RR42_RS23400 0.65 -0.1 578,010 + RR42_RS23400 0.65 -1.7 578,010 + RR42_RS23400 0.65 -0.2 578,011 - RR42_RS23400 0.65 +0.1 578,011 - RR42_RS23400 0.65 +0.5 578,157 - RR42_RS23400 0.80 +0.2 578,244 - RR42_RS23400 0.89 +0.4 578,613 + +0.3 578,614 - +2.2 578,661 + -0.2 578,750 - RR42_RS23405 0.15 -0.5 578,837 + RR42_RS23405 0.24 +0.6 578,838 - RR42_RS23405 0.24 -0.1 578,876 - RR42_RS23405 0.29 +0.7 578,924 + RR42_RS23405 0.34 +0.3 579,201 + RR42_RS23405 0.65 +0.1 579,202 - RR42_RS23405 0.65 +0.5 579,327 + RR42_RS23405 0.79 +1.1 579,327 + RR42_RS23405 0.79 +1.2 579,328 - RR42_RS23405 0.79 -0.0 579,328 - RR42_RS23405 0.79 -0.7
Or see this region's nucleotide sequence