Experiment: R2A with A22 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS23290 and RR42_RS23295 are separated by 32 nucleotides RR42_RS23295 and RR42_RS23300 are separated by 29 nucleotides RR42_RS23300 and RR42_RS23305 are separated by 93 nucleotides RR42_RS23305 and RR42_RS23310 overlap by 4 nucleotides
RR42_RS23290: RR42_RS23290 - hypothetical protein, at 555,575 to 557,095
_RS23290
RR42_RS23295: RR42_RS23295 - hypothetical protein, at 557,128 to 557,865
_RS23295
RR42_RS23300: RR42_RS23300 - hypothetical protein, at 557,895 to 558,860
_RS23300
RR42_RS23305: RR42_RS23305 - hypothetical protein, at 558,954 to 559,529
_RS23305
RR42_RS23310: RR42_RS23310 - cytochrome B6, at 559,526 to 560,863
_RS23310
Position (kb) 557
558
559 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 557.093 kb on + strand at 557.094 kb on - strand at 557.100 kb on - strand at 557.127 kb on + strand at 557.127 kb on + strand at 557.128 kb on - strand at 557.128 kb on - strand at 557.128 kb on - strand at 557.514 kb on + strand, within RR42_RS23295 at 557.762 kb on - strand, within RR42_RS23295 at 557.762 kb on - strand, within RR42_RS23295 at 557.990 kb on + strand at 557.990 kb on + strand at 557.991 kb on - strand at 557.991 kb on - strand at 558.052 kb on + strand, within RR42_RS23300 at 558.053 kb on - strand, within RR42_RS23300 at 558.053 kb on - strand, within RR42_RS23300 at 558.392 kb on - strand, within RR42_RS23300 at 558.392 kb on - strand, within RR42_RS23300 at 558.392 kb on - strand, within RR42_RS23300 at 558.460 kb on - strand, within RR42_RS23300 at 558.460 kb on - strand, within RR42_RS23300 at 558.572 kb on - strand, within RR42_RS23300 at 558.623 kb on + strand, within RR42_RS23300 at 558.788 kb on + strand at 558.789 kb on - strand at 558.793 kb on - strand at 558.920 kb on - strand at 558.920 kb on - strand at 559.317 kb on + strand, within RR42_RS23305 at 559.365 kb on + strand, within RR42_RS23305 at 559.566 kb on + strand at 559.567 kb on - strand at 559.567 kb on - strand at 559.761 kb on + strand, within RR42_RS23310 at 559.762 kb on - strand, within RR42_RS23310 at 559.762 kb on - strand, within RR42_RS23310 at 559.762 kb on - strand, within RR42_RS23310
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.01 mg/ml remove 557,093 + -2.5 557,094 - -0.0 557,100 - -0.6 557,127 + -0.7 557,127 + -0.5 557,128 - +1.3 557,128 - +0.5 557,128 - +0.3 557,514 + RR42_RS23295 0.52 -1.2 557,762 - RR42_RS23295 0.86 +0.3 557,762 - RR42_RS23295 0.86 -0.1 557,990 + -0.2 557,990 + -0.9 557,991 - +0.1 557,991 - -0.1 558,052 + RR42_RS23300 0.16 +0.2 558,053 - RR42_RS23300 0.16 +0.1 558,053 - RR42_RS23300 0.16 -0.9 558,392 - RR42_RS23300 0.51 -0.6 558,392 - RR42_RS23300 0.51 -0.5 558,392 - RR42_RS23300 0.51 +0.9 558,460 - RR42_RS23300 0.58 +0.1 558,460 - RR42_RS23300 0.58 -0.6 558,572 - RR42_RS23300 0.70 -0.2 558,623 + RR42_RS23300 0.75 +0.6 558,788 + +0.8 558,789 - -0.2 558,793 - -0.2 558,920 - -0.1 558,920 - +0.6 559,317 + RR42_RS23305 0.63 +0.1 559,365 + RR42_RS23305 0.71 -1.4 559,566 + -0.4 559,567 - +0.3 559,567 - +0.2 559,761 + RR42_RS23310 0.18 -3.5 559,762 - RR42_RS23310 0.18 +0.1 559,762 - RR42_RS23310 0.18 -0.4 559,762 - RR42_RS23310 0.18 +0.6
Or see this region's nucleotide sequence