Experiment: R2A with A22 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS23195 and RR42_RS23200 are separated by 94 nucleotides RR42_RS23200 and RR42_RS23205 are separated by 91 nucleotides RR42_RS23205 and RR42_RS23210 are separated by 242 nucleotides RR42_RS23210 and RR42_RS23215 are separated by 17 nucleotides
RR42_RS23195: RR42_RS23195 - membrane protein, at 537,471 to 538,349
_RS23195
RR42_RS23200: RR42_RS23200 - aldehyde-activating protein, at 538,444 to 538,884
_RS23200
RR42_RS23205: RR42_RS23205 - hypothetical protein, at 538,976 to 539,365
_RS23205
RR42_RS23210: RR42_RS23210 - GntR family transcriptional regulator, at 539,608 to 540,285
_RS23210
RR42_RS23215: RR42_RS23215 - DSBA oxidoreductase, at 540,303 to 541,841
_RS23215
Position (kb) 538
539
540 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 538.112 kb on - strand, within RR42_RS23195 at 538.147 kb on - strand, within RR42_RS23195 at 538.159 kb on + strand, within RR42_RS23195 at 538.159 kb on + strand, within RR42_RS23195 at 538.159 kb on + strand, within RR42_RS23195 at 538.160 kb on - strand, within RR42_RS23195 at 538.163 kb on + strand, within RR42_RS23195 at 538.163 kb on + strand, within RR42_RS23195 at 538.163 kb on + strand, within RR42_RS23195 at 538.163 kb on + strand, within RR42_RS23195 at 538.163 kb on + strand, within RR42_RS23195 at 538.164 kb on - strand, within RR42_RS23195 at 538.197 kb on + strand, within RR42_RS23195 at 538.197 kb on + strand, within RR42_RS23195 at 538.197 kb on + strand, within RR42_RS23195 at 538.198 kb on - strand, within RR42_RS23195 at 538.298 kb on - strand at 538.348 kb on - strand at 538.398 kb on + strand at 538.398 kb on + strand at 538.422 kb on + strand at 538.487 kb on + strand at 538.487 kb on + strand at 538.488 kb on - strand, within RR42_RS23200 at 538.488 kb on - strand, within RR42_RS23200 at 538.529 kb on + strand, within RR42_RS23200 at 538.530 kb on - strand, within RR42_RS23200 at 538.694 kb on + strand, within RR42_RS23200 at 538.694 kb on + strand, within RR42_RS23200 at 538.694 kb on + strand, within RR42_RS23200 at 538.694 kb on + strand, within RR42_RS23200 at 538.695 kb on - strand, within RR42_RS23200 at 538.695 kb on - strand, within RR42_RS23200 at 538.695 kb on - strand, within RR42_RS23200 at 538.695 kb on - strand, within RR42_RS23200 at 538.775 kb on + strand, within RR42_RS23200 at 538.778 kb on - strand, within RR42_RS23200 at 538.870 kb on - strand at 538.884 kb on + strand at 538.885 kb on - strand at 538.891 kb on + strand at 538.891 kb on + strand at 538.891 kb on + strand at 538.933 kb on + strand at 538.933 kb on + strand at 538.934 kb on - strand at 538.934 kb on - strand at 538.934 kb on - strand at 538.934 kb on - strand at 538.934 kb on - strand at 538.934 kb on - strand at 538.978 kb on - strand at 539.055 kb on - strand, within RR42_RS23205 at 539.416 kb on + strand at 539.417 kb on - strand at 539.417 kb on - strand at 539.417 kb on - strand at 539.418 kb on + strand at 539.418 kb on + strand at 539.418 kb on + strand at 539.418 kb on + strand at 539.418 kb on + strand at 539.419 kb on - strand at 539.419 kb on - strand at 539.419 kb on - strand at 539.485 kb on - strand at 539.525 kb on + strand at 539.525 kb on + strand at 539.526 kb on - strand at 539.526 kb on - strand at 539.599 kb on + strand at 539.599 kb on + strand at 539.599 kb on + strand at 539.599 kb on + strand at 539.600 kb on - strand at 539.960 kb on - strand, within RR42_RS23210 at 540.064 kb on + strand, within RR42_RS23210 at 540.064 kb on + strand, within RR42_RS23210 at 540.064 kb on + strand, within RR42_RS23210 at 540.065 kb on - strand, within RR42_RS23210
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.01 mg/ml remove 538,112 - RR42_RS23195 0.73 -0.3 538,147 - RR42_RS23195 0.77 +0.6 538,159 + RR42_RS23195 0.78 -0.3 538,159 + RR42_RS23195 0.78 -0.5 538,159 + RR42_RS23195 0.78 -0.1 538,160 - RR42_RS23195 0.78 -0.3 538,163 + RR42_RS23195 0.79 -0.5 538,163 + RR42_RS23195 0.79 -0.7 538,163 + RR42_RS23195 0.79 -0.1 538,163 + RR42_RS23195 0.79 +0.6 538,163 + RR42_RS23195 0.79 +0.5 538,164 - RR42_RS23195 0.79 -0.7 538,197 + RR42_RS23195 0.83 +0.5 538,197 + RR42_RS23195 0.83 -0.5 538,197 + RR42_RS23195 0.83 +0.2 538,198 - RR42_RS23195 0.83 -1.3 538,298 - -0.9 538,348 - -0.0 538,398 + +0.2 538,398 + +0.2 538,422 + +0.3 538,487 + +1.1 538,487 + -2.2 538,488 - RR42_RS23200 0.10 -1.0 538,488 - RR42_RS23200 0.10 +0.1 538,529 + RR42_RS23200 0.19 +0.2 538,530 - RR42_RS23200 0.20 +0.3 538,694 + RR42_RS23200 0.57 -0.5 538,694 + RR42_RS23200 0.57 -0.2 538,694 + RR42_RS23200 0.57 -1.7 538,694 + RR42_RS23200 0.57 +0.0 538,695 - RR42_RS23200 0.57 +0.1 538,695 - RR42_RS23200 0.57 +0.3 538,695 - RR42_RS23200 0.57 +0.4 538,695 - RR42_RS23200 0.57 -0.9 538,775 + RR42_RS23200 0.75 +0.1 538,778 - RR42_RS23200 0.76 -0.2 538,870 - +0.0 538,884 + -2.9 538,885 - -1.1 538,891 + +0.3 538,891 + -1.4 538,891 + +0.1 538,933 + -2.1 538,933 + -0.7 538,934 - -0.6 538,934 - -0.7 538,934 - -0.3 538,934 - +0.1 538,934 - -0.4 538,934 - +0.8 538,978 - -1.5 539,055 - RR42_RS23205 0.20 -0.7 539,416 + -0.0 539,417 - +0.3 539,417 - +0.6 539,417 - -1.6 539,418 + -0.4 539,418 + -0.3 539,418 + -0.3 539,418 + +0.3 539,418 + -0.8 539,419 - -0.5 539,419 - +1.0 539,419 - -0.4 539,485 - +0.3 539,525 + +1.2 539,525 + +0.7 539,526 - -0.9 539,526 - +1.1 539,599 + +0.6 539,599 + +0.0 539,599 + -1.2 539,599 + -0.3 539,600 - -0.6 539,960 - RR42_RS23210 0.52 +0.4 540,064 + RR42_RS23210 0.67 -0.5 540,064 + RR42_RS23210 0.67 -0.3 540,064 + RR42_RS23210 0.67 +0.4 540,065 - RR42_RS23210 0.67 -0.5
Or see this region's nucleotide sequence