Experiment: R2A with A22 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS23065 and RR42_RS23070 are separated by 162 nucleotides RR42_RS23070 and RR42_RS23075 are separated by 31 nucleotides
RR42_RS23065: RR42_RS23065 - L-rhamnose 1-epimerase, at 509,085 to 510,260
_RS23065
RR42_RS23070: RR42_RS23070 - 4-hydroxythreonine-4-phosphate dehydrogenase, at 510,423 to 511,454
_RS23070
RR42_RS23075: RR42_RS23075 - ABC transporter substrate-binding protein, at 511,486 to 512,451
_RS23075
Position (kb)
510
511
512 Strain fitness (log2 ratio)
-2
-1
0
1 at 509.443 kb on + strand, within RR42_RS23065 at 509.443 kb on + strand, within RR42_RS23065 at 509.506 kb on + strand, within RR42_RS23065 at 509.609 kb on + strand, within RR42_RS23065 at 509.610 kb on - strand, within RR42_RS23065 at 509.611 kb on + strand, within RR42_RS23065 at 509.611 kb on + strand, within RR42_RS23065 at 509.611 kb on + strand, within RR42_RS23065 at 509.611 kb on + strand, within RR42_RS23065 at 509.611 kb on + strand, within RR42_RS23065 at 509.612 kb on - strand, within RR42_RS23065 at 509.612 kb on - strand, within RR42_RS23065 at 509.612 kb on - strand, within RR42_RS23065 at 509.612 kb on - strand, within RR42_RS23065 at 509.612 kb on - strand, within RR42_RS23065 at 509.612 kb on - strand, within RR42_RS23065 at 509.612 kb on - strand, within RR42_RS23065 at 509.612 kb on - strand, within RR42_RS23065 at 509.755 kb on + strand, within RR42_RS23065 at 509.755 kb on + strand, within RR42_RS23065 at 509.756 kb on - strand, within RR42_RS23065 at 509.801 kb on - strand, within RR42_RS23065 at 509.801 kb on - strand, within RR42_RS23065 at 509.815 kb on + strand, within RR42_RS23065 at 509.815 kb on + strand, within RR42_RS23065 at 509.815 kb on + strand, within RR42_RS23065 at 509.815 kb on + strand, within RR42_RS23065 at 509.816 kb on - strand, within RR42_RS23065 at 509.816 kb on - strand, within RR42_RS23065 at 509.816 kb on - strand, within RR42_RS23065 at 509.965 kb on + strand, within RR42_RS23065 at 509.965 kb on + strand, within RR42_RS23065 at 509.966 kb on - strand, within RR42_RS23065 at 509.983 kb on + strand, within RR42_RS23065 at 509.984 kb on - strand, within RR42_RS23065 at 509.984 kb on - strand, within RR42_RS23065 at 510.028 kb on + strand, within RR42_RS23065 at 510.029 kb on - strand, within RR42_RS23065 at 510.029 kb on - strand, within RR42_RS23065 at 510.424 kb on + strand at 510.634 kb on + strand, within RR42_RS23070 at 510.635 kb on - strand, within RR42_RS23070 at 510.635 kb on - strand, within RR42_RS23070 at 510.635 kb on - strand, within RR42_RS23070 at 510.635 kb on - strand, within RR42_RS23070 at 511.040 kb on + strand, within RR42_RS23070 at 511.154 kb on - strand, within RR42_RS23070 at 511.573 kb on - strand at 511.694 kb on + strand, within RR42_RS23075 at 511.695 kb on - strand, within RR42_RS23075 at 511.821 kb on + strand, within RR42_RS23075 at 511.821 kb on + strand, within RR42_RS23075 at 511.822 kb on - strand, within RR42_RS23075 at 511.907 kb on + strand, within RR42_RS23075 at 511.908 kb on - strand, within RR42_RS23075 at 511.908 kb on - strand, within RR42_RS23075 at 511.992 kb on + strand, within RR42_RS23075 at 511.992 kb on + strand, within RR42_RS23075 at 511.992 kb on + strand, within RR42_RS23075 at 511.992 kb on + strand, within RR42_RS23075 at 511.993 kb on - strand, within RR42_RS23075 at 512.221 kb on - strand, within RR42_RS23075 at 512.235 kb on + strand, within RR42_RS23075 at 512.235 kb on + strand, within RR42_RS23075 at 512.236 kb on - strand, within RR42_RS23075 at 512.236 kb on - strand, within RR42_RS23075 at 512.236 kb on - strand, within RR42_RS23075 at 512.385 kb on - strand at 512.452 kb on + strand at 512.452 kb on + strand at 512.452 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.01 mg/ml remove 509,443 + RR42_RS23065 0.30 +0.1 509,443 + RR42_RS23065 0.30 -2.1 509,506 + RR42_RS23065 0.36 +0.3 509,609 + RR42_RS23065 0.45 +0.5 509,610 - RR42_RS23065 0.45 +0.1 509,611 + RR42_RS23065 0.45 -1.5 509,611 + RR42_RS23065 0.45 -0.5 509,611 + RR42_RS23065 0.45 +0.1 509,611 + RR42_RS23065 0.45 +0.2 509,611 + RR42_RS23065 0.45 -1.5 509,612 - RR42_RS23065 0.45 +0.9 509,612 - RR42_RS23065 0.45 -1.2 509,612 - RR42_RS23065 0.45 -0.6 509,612 - RR42_RS23065 0.45 +0.5 509,612 - RR42_RS23065 0.45 -0.2 509,612 - RR42_RS23065 0.45 +0.6 509,612 - RR42_RS23065 0.45 -0.6 509,612 - RR42_RS23065 0.45 +0.6 509,755 + RR42_RS23065 0.57 -0.0 509,755 + RR42_RS23065 0.57 +0.2 509,756 - RR42_RS23065 0.57 -0.4 509,801 - RR42_RS23065 0.61 -0.3 509,801 - RR42_RS23065 0.61 -0.9 509,815 + RR42_RS23065 0.62 -1.3 509,815 + RR42_RS23065 0.62 +0.4 509,815 + RR42_RS23065 0.62 +0.8 509,815 + RR42_RS23065 0.62 -0.2 509,816 - RR42_RS23065 0.62 -0.3 509,816 - RR42_RS23065 0.62 +1.1 509,816 - RR42_RS23065 0.62 +0.3 509,965 + RR42_RS23065 0.75 +0.7 509,965 + RR42_RS23065 0.75 +0.2 509,966 - RR42_RS23065 0.75 +0.2 509,983 + RR42_RS23065 0.76 -0.1 509,984 - RR42_RS23065 0.76 +0.4 509,984 - RR42_RS23065 0.76 +0.4 510,028 + RR42_RS23065 0.80 -0.5 510,029 - RR42_RS23065 0.80 +0.7 510,029 - RR42_RS23065 0.80 +0.2 510,424 + +1.0 510,634 + RR42_RS23070 0.20 -0.8 510,635 - RR42_RS23070 0.21 +0.7 510,635 - RR42_RS23070 0.21 -0.5 510,635 - RR42_RS23070 0.21 +0.8 510,635 - RR42_RS23070 0.21 -1.6 511,040 + RR42_RS23070 0.60 -0.9 511,154 - RR42_RS23070 0.71 -0.6 511,573 - -0.6 511,694 + RR42_RS23075 0.22 -0.9 511,695 - RR42_RS23075 0.22 -0.2 511,821 + RR42_RS23075 0.35 +0.2 511,821 + RR42_RS23075 0.35 +0.4 511,822 - RR42_RS23075 0.35 +0.4 511,907 + RR42_RS23075 0.44 -0.1 511,908 - RR42_RS23075 0.44 +0.2 511,908 - RR42_RS23075 0.44 +0.9 511,992 + RR42_RS23075 0.52 -0.9 511,992 + RR42_RS23075 0.52 +0.9 511,992 + RR42_RS23075 0.52 -0.5 511,992 + RR42_RS23075 0.52 -0.8 511,993 - RR42_RS23075 0.52 -0.7 512,221 - RR42_RS23075 0.76 -1.5 512,235 + RR42_RS23075 0.78 -2.0 512,235 + RR42_RS23075 0.78 +0.2 512,236 - RR42_RS23075 0.78 +0.9 512,236 - RR42_RS23075 0.78 +1.6 512,236 - RR42_RS23075 0.78 +0.2 512,385 - -0.7 512,452 + -0.7 512,452 + +0.3 512,452 + +1.1
Or see this region's nucleotide sequence