Experiment: R2A with A22 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS22465 and RR42_RS22470 are separated by 173 nucleotides RR42_RS22470 and RR42_RS22475 are separated by 101 nucleotides RR42_RS22475 and RR42_RS22480 are separated by 35 nucleotides
RR42_RS22465: RR42_RS22465 - membrane protein, at 375,940 to 377,994
_RS22465
RR42_RS22470: RR42_RS22470 - MFS transporter, at 378,168 to 379,493
_RS22470
RR42_RS22475: RR42_RS22475 - hypothetical protein, at 379,595 to 379,810
_RS22475
RR42_RS22480: RR42_RS22480 - FAD-dependent oxidoreductase, at 379,846 to 381,579
_RS22480
Position (kb) 378
379
380 Strain fitness (log2 ratio) -2
-1
0
1
2 at 377.214 kb on - strand, within RR42_RS22465 at 377.427 kb on - strand, within RR42_RS22465 at 377.585 kb on + strand, within RR42_RS22465 at 377.585 kb on + strand, within RR42_RS22465 at 377.585 kb on + strand, within RR42_RS22465 at 377.586 kb on - strand, within RR42_RS22465 at 377.588 kb on + strand, within RR42_RS22465 at 377.588 kb on + strand, within RR42_RS22465 at 377.588 kb on + strand, within RR42_RS22465 at 377.589 kb on - strand, within RR42_RS22465 at 377.589 kb on - strand, within RR42_RS22465 at 377.627 kb on + strand, within RR42_RS22465 at 377.628 kb on - strand, within RR42_RS22465 at 377.927 kb on + strand at 377.928 kb on - strand at 377.978 kb on + strand at 377.979 kb on - strand at 377.983 kb on - strand at 378.244 kb on + strand at 378.244 kb on + strand at 378.245 kb on - strand at 378.245 kb on - strand at 378.245 kb on - strand at 378.335 kb on + strand, within RR42_RS22470 at 378.608 kb on - strand, within RR42_RS22470 at 378.707 kb on + strand, within RR42_RS22470 at 378.707 kb on + strand, within RR42_RS22470 at 378.708 kb on - strand, within RR42_RS22470 at 378.721 kb on + strand, within RR42_RS22470 at 378.722 kb on - strand, within RR42_RS22470 at 378.934 kb on + strand, within RR42_RS22470 at 378.935 kb on - strand, within RR42_RS22470 at 379.073 kb on - strand, within RR42_RS22470 at 379.073 kb on - strand, within RR42_RS22470 at 379.073 kb on - strand, within RR42_RS22470 at 379.133 kb on - strand, within RR42_RS22470 at 379.133 kb on - strand, within RR42_RS22470 at 379.274 kb on - strand, within RR42_RS22470 at 379.274 kb on - strand, within RR42_RS22470 at 379.274 kb on - strand, within RR42_RS22470 at 379.274 kb on - strand, within RR42_RS22470 at 379.298 kb on - strand, within RR42_RS22470 at 379.376 kb on + strand at 379.377 kb on - strand at 379.381 kb on + strand at 379.381 kb on + strand at 379.382 kb on - strand at 379.382 kb on - strand at 379.382 kb on - strand at 379.998 kb on - strand at 380.022 kb on - strand, within RR42_RS22480 at 380.203 kb on + strand, within RR42_RS22480 at 380.204 kb on - strand, within RR42_RS22480 at 380.356 kb on - strand, within RR42_RS22480 at 380.356 kb on - strand, within RR42_RS22480 at 380.493 kb on - strand, within RR42_RS22480
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.01 mg/ml remove 377,214 - RR42_RS22465 0.62 -0.6 377,427 - RR42_RS22465 0.72 +1.8 377,585 + RR42_RS22465 0.80 -0.2 377,585 + RR42_RS22465 0.80 +0.4 377,585 + RR42_RS22465 0.80 +1.0 377,586 - RR42_RS22465 0.80 -0.4 377,588 + RR42_RS22465 0.80 -0.2 377,588 + RR42_RS22465 0.80 -0.8 377,588 + RR42_RS22465 0.80 -0.6 377,589 - RR42_RS22465 0.80 +1.4 377,589 - RR42_RS22465 0.80 -0.7 377,627 + RR42_RS22465 0.82 +0.0 377,628 - RR42_RS22465 0.82 +1.3 377,927 + +0.3 377,928 - -1.0 377,978 + +1.4 377,979 - +0.4 377,983 - -2.1 378,244 + -0.3 378,244 + -0.1 378,245 - +0.2 378,245 - +1.2 378,245 - -1.5 378,335 + RR42_RS22470 0.13 +1.2 378,608 - RR42_RS22470 0.33 -0.2 378,707 + RR42_RS22470 0.41 -0.1 378,707 + RR42_RS22470 0.41 -0.1 378,708 - RR42_RS22470 0.41 -1.1 378,721 + RR42_RS22470 0.42 +1.9 378,722 - RR42_RS22470 0.42 +0.4 378,934 + RR42_RS22470 0.58 -0.9 378,935 - RR42_RS22470 0.58 +0.6 379,073 - RR42_RS22470 0.68 +0.2 379,073 - RR42_RS22470 0.68 +1.3 379,073 - RR42_RS22470 0.68 +0.3 379,133 - RR42_RS22470 0.73 +0.5 379,133 - RR42_RS22470 0.73 +1.1 379,274 - RR42_RS22470 0.83 -1.6 379,274 - RR42_RS22470 0.83 -0.1 379,274 - RR42_RS22470 0.83 -0.2 379,274 - RR42_RS22470 0.83 +0.9 379,298 - RR42_RS22470 0.85 -0.7 379,376 + +1.1 379,377 - +0.3 379,381 + +0.9 379,381 + +0.4 379,382 - +0.6 379,382 - -1.6 379,382 - -0.7 379,998 - +0.2 380,022 - RR42_RS22480 0.10 +0.2 380,203 + RR42_RS22480 0.21 -0.2 380,204 - RR42_RS22480 0.21 -0.9 380,356 - RR42_RS22480 0.29 +1.1 380,356 - RR42_RS22480 0.29 +0.7 380,493 - RR42_RS22480 0.37 +0.6
Or see this region's nucleotide sequence