Experiment: R2A with A22 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS22060 and RR42_RS22065 are separated by 25 nucleotides RR42_RS22065 and RR42_RS22070 are separated by 233 nucleotides RR42_RS22070 and RR42_RS22075 are separated by 93 nucleotides
RR42_RS22060: RR42_RS22060 - type VI secretion protein EvpB, at 282,821 to 284,368
_RS22060
RR42_RS22065: RR42_RS22065 - type VI secretion protein, at 284,394 to 284,909
_RS22065
RR42_RS22070: RR42_RS22070 - type IV secretion protein Rhs, at 285,143 to 287,623
_RS22070
RR42_RS22075: RR42_RS22075 - hypothetical protein, at 287,717 to 288,985
_RS22075
Position (kb) 285
286
287
288 Strain fitness (log2 ratio) -1
0
1
2 at 284.289 kb on + strand at 284.396 kb on - strand at 284.788 kb on + strand, within RR42_RS22065 at 284.789 kb on - strand, within RR42_RS22065 at 284.865 kb on - strand at 284.971 kb on - strand at 284.988 kb on + strand at 284.988 kb on + strand at 285.013 kb on + strand at 285.013 kb on + strand at 285.018 kb on - strand at 285.027 kb on - strand at 285.078 kb on + strand at 285.170 kb on + strand at 285.210 kb on - strand at 285.210 kb on - strand at 285.967 kb on + strand, within RR42_RS22070 at 285.967 kb on + strand, within RR42_RS22070 at 286.055 kb on + strand, within RR42_RS22070 at 287.404 kb on + strand at 287.405 kb on - strand at 287.405 kb on - strand at 287.410 kb on + strand at 287.411 kb on - strand at 287.411 kb on - strand at 287.441 kb on + strand at 287.441 kb on + strand at 287.489 kb on + strand at 287.519 kb on - strand at 287.624 kb on + strand at 287.945 kb on + strand, within RR42_RS22075 at 287.946 kb on - strand, within RR42_RS22075 at 287.946 kb on - strand, within RR42_RS22075 at 287.946 kb on - strand, within RR42_RS22075 at 288.193 kb on + strand, within RR42_RS22075 at 288.274 kb on - strand, within RR42_RS22075 at 288.367 kb on - strand, within RR42_RS22075 at 288.367 kb on - strand, within RR42_RS22075
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.01 mg/ml remove 284,289 + -0.7 284,396 - -0.7 284,788 + RR42_RS22065 0.76 -1.3 284,789 - RR42_RS22065 0.77 -0.3 284,865 - +1.1 284,971 - +0.5 284,988 + -0.5 284,988 + -0.3 285,013 + +2.1 285,013 + -0.6 285,018 - -0.2 285,027 - +0.8 285,078 + -0.1 285,170 + -1.5 285,210 - +0.0 285,210 - -0.9 285,967 + RR42_RS22070 0.33 +0.4 285,967 + RR42_RS22070 0.33 +1.9 286,055 + RR42_RS22070 0.37 +0.5 287,404 + -0.0 287,405 - +0.3 287,405 - -0.4 287,410 + +0.3 287,411 - +0.1 287,411 - -1.0 287,441 + +0.4 287,441 + +0.1 287,489 + +0.5 287,519 - +0.1 287,624 + -0.1 287,945 + RR42_RS22075 0.18 +1.7 287,946 - RR42_RS22075 0.18 -0.8 287,946 - RR42_RS22075 0.18 -0.9 287,946 - RR42_RS22075 0.18 +0.4 288,193 + RR42_RS22075 0.38 -0.3 288,274 - RR42_RS22075 0.44 +0.1 288,367 - RR42_RS22075 0.51 -0.7 288,367 - RR42_RS22075 0.51 -0.1
Or see this region's nucleotide sequence