Experiment: R2A with A22 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS22060 and RR42_RS22065 are separated by 25 nucleotides RR42_RS22065 and RR42_RS22070 are separated by 233 nucleotides
RR42_RS22060: RR42_RS22060 - type VI secretion protein EvpB, at 282,821 to 284,368
_RS22060
RR42_RS22065: RR42_RS22065 - type VI secretion protein, at 284,394 to 284,909
_RS22065
RR42_RS22070: RR42_RS22070 - type IV secretion protein Rhs, at 285,143 to 287,623
_RS22070
Position (kb)
284
285 Strain fitness (log2 ratio)
-1
0
1
2 at 283.515 kb on + strand, within RR42_RS22060 at 283.594 kb on - strand, within RR42_RS22060 at 283.594 kb on - strand, within RR42_RS22060 at 283.594 kb on - strand, within RR42_RS22060 at 283.594 kb on - strand, within RR42_RS22060 at 283.594 kb on - strand, within RR42_RS22060 at 283.635 kb on + strand, within RR42_RS22060 at 283.738 kb on + strand, within RR42_RS22060 at 283.739 kb on - strand, within RR42_RS22060 at 283.766 kb on - strand, within RR42_RS22060 at 283.867 kb on - strand, within RR42_RS22060 at 284.289 kb on + strand at 284.396 kb on - strand at 284.788 kb on + strand, within RR42_RS22065 at 284.789 kb on - strand, within RR42_RS22065 at 284.865 kb on - strand at 284.971 kb on - strand at 284.988 kb on + strand at 284.988 kb on + strand at 285.013 kb on + strand at 285.013 kb on + strand at 285.018 kb on - strand at 285.027 kb on - strand at 285.078 kb on + strand at 285.170 kb on + strand at 285.210 kb on - strand at 285.210 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.01 mg/ml remove 283,515 + RR42_RS22060 0.45 +0.3 283,594 - RR42_RS22060 0.50 -0.2 283,594 - RR42_RS22060 0.50 -0.1 283,594 - RR42_RS22060 0.50 -0.8 283,594 - RR42_RS22060 0.50 +0.4 283,594 - RR42_RS22060 0.50 -0.2 283,635 + RR42_RS22060 0.53 +0.3 283,738 + RR42_RS22060 0.59 -0.6 283,739 - RR42_RS22060 0.59 -0.4 283,766 - RR42_RS22060 0.61 +0.5 283,867 - RR42_RS22060 0.68 -0.6 284,289 + -0.7 284,396 - -0.7 284,788 + RR42_RS22065 0.76 -1.3 284,789 - RR42_RS22065 0.77 -0.3 284,865 - +1.1 284,971 - +0.5 284,988 + -0.5 284,988 + -0.3 285,013 + +2.1 285,013 + -0.6 285,018 - -0.2 285,027 - +0.8 285,078 + -0.1 285,170 + -1.5 285,210 - +0.0 285,210 - -0.9
Or see this region's nucleotide sequence