Experiment: R2A with A22 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS21830 and RR42_RS21835 are separated by 116 nucleotides RR42_RS21835 and RR42_RS21840 are separated by 37 nucleotides RR42_RS21840 and RR42_RS21845 are separated by 109 nucleotides
RR42_RS21830: RR42_RS21830 - transcriptional regulator, at 225,965 to 226,879
_RS21830
RR42_RS21835: RR42_RS21835 - muconate cycloisomerase, at 226,996 to 228,117
_RS21835
RR42_RS21840: RR42_RS21840 - muconolactone delta-isomerase, at 228,155 to 228,433
_RS21840
RR42_RS21845: RR42_RS21845 - LacI family transcriptional regulator, at 228,543 to 229,529
_RS21845
Position (kb)
226
227
228
229 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 226.040 kb on + strand at 226.066 kb on - strand, within RR42_RS21830 at 226.066 kb on - strand, within RR42_RS21830 at 226.276 kb on - strand, within RR42_RS21830 at 226.276 kb on - strand, within RR42_RS21830 at 226.491 kb on - strand, within RR42_RS21830 at 226.491 kb on - strand, within RR42_RS21830 at 226.572 kb on + strand, within RR42_RS21830 at 226.573 kb on - strand, within RR42_RS21830 at 226.801 kb on + strand at 226.802 kb on - strand at 226.857 kb on + strand at 226.857 kb on + strand at 226.857 kb on + strand at 226.871 kb on + strand at 226.871 kb on + strand at 226.872 kb on - strand at 226.872 kb on - strand at 226.872 kb on - strand at 226.872 kb on - strand at 226.920 kb on + strand at 226.921 kb on - strand at 226.921 kb on - strand at 226.921 kb on - strand at 226.955 kb on + strand at 226.956 kb on - strand at 226.956 kb on - strand at 226.956 kb on - strand at 226.956 kb on - strand at 226.971 kb on - strand at 226.971 kb on - strand at 226.977 kb on + strand at 226.977 kb on + strand at 226.978 kb on - strand at 227.167 kb on + strand, within RR42_RS21835 at 227.167 kb on + strand, within RR42_RS21835 at 227.167 kb on + strand, within RR42_RS21835 at 227.209 kb on - strand, within RR42_RS21835 at 227.212 kb on + strand, within RR42_RS21835 at 227.213 kb on - strand, within RR42_RS21835 at 227.213 kb on - strand, within RR42_RS21835 at 228.025 kb on + strand at 228.180 kb on + strand at 228.180 kb on + strand at 228.180 kb on + strand at 228.180 kb on + strand at 228.180 kb on + strand at 228.180 kb on + strand at 228.181 kb on - strand at 228.181 kb on - strand at 228.181 kb on - strand at 228.302 kb on + strand, within RR42_RS21840 at 228.302 kb on + strand, within RR42_RS21840 at 228.302 kb on + strand, within RR42_RS21840 at 228.311 kb on + strand, within RR42_RS21840 at 228.311 kb on + strand, within RR42_RS21840 at 228.312 kb on - strand, within RR42_RS21840 at 228.312 kb on - strand, within RR42_RS21840 at 228.312 kb on - strand, within RR42_RS21840 at 228.380 kb on + strand, within RR42_RS21840 at 228.380 kb on + strand, within RR42_RS21840 at 228.381 kb on - strand, within RR42_RS21840 at 228.432 kb on - strand at 228.554 kb on - strand at 228.633 kb on + strand at 228.633 kb on + strand at 228.634 kb on - strand at 228.880 kb on - strand, within RR42_RS21845 at 228.930 kb on - strand, within RR42_RS21845 at 229.014 kb on + strand, within RR42_RS21845 at 229.014 kb on + strand, within RR42_RS21845 at 229.014 kb on + strand, within RR42_RS21845 at 229.014 kb on + strand, within RR42_RS21845 at 229.015 kb on - strand, within RR42_RS21845 at 229.020 kb on + strand, within RR42_RS21845 at 229.020 kb on + strand, within RR42_RS21845 at 229.020 kb on + strand, within RR42_RS21845 at 229.020 kb on + strand, within RR42_RS21845 at 229.020 kb on + strand, within RR42_RS21845 at 229.020 kb on + strand, within RR42_RS21845 at 229.021 kb on - strand, within RR42_RS21845 at 229.021 kb on - strand, within RR42_RS21845 at 229.021 kb on - strand, within RR42_RS21845 at 229.021 kb on - strand, within RR42_RS21845 at 229.021 kb on - strand, within RR42_RS21845 at 229.021 kb on - strand, within RR42_RS21845 at 229.108 kb on - strand, within RR42_RS21845
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.01 mg/ml remove 226,040 + +1.1 226,066 - RR42_RS21830 0.11 +0.2 226,066 - RR42_RS21830 0.11 -1.0 226,276 - RR42_RS21830 0.34 +1.1 226,276 - RR42_RS21830 0.34 -0.4 226,491 - RR42_RS21830 0.57 -0.8 226,491 - RR42_RS21830 0.57 -0.8 226,572 + RR42_RS21830 0.66 -0.9 226,573 - RR42_RS21830 0.66 +2.1 226,801 + -1.3 226,802 - -1.7 226,857 + +1.3 226,857 + -1.0 226,857 + +0.7 226,871 + +0.6 226,871 + -1.3 226,872 - +0.1 226,872 - -0.5 226,872 - +0.4 226,872 - -0.5 226,920 + -0.4 226,921 - +0.7 226,921 - -1.0 226,921 - -1.9 226,955 + +0.2 226,956 - +0.4 226,956 - +1.3 226,956 - -1.7 226,956 - -0.1 226,971 - -0.6 226,971 - -0.2 226,977 + -0.7 226,977 + +1.9 226,978 - -0.5 227,167 + RR42_RS21835 0.15 -0.5 227,167 + RR42_RS21835 0.15 +2.1 227,167 + RR42_RS21835 0.15 -0.2 227,209 - RR42_RS21835 0.19 +1.2 227,212 + RR42_RS21835 0.19 +0.4 227,213 - RR42_RS21835 0.19 +0.5 227,213 - RR42_RS21835 0.19 +0.2 228,025 + -0.2 228,180 + -1.5 228,180 + -1.2 228,180 + -1.6 228,180 + -0.5 228,180 + +0.3 228,180 + +0.8 228,181 - -0.0 228,181 - -0.2 228,181 - +0.1 228,302 + RR42_RS21840 0.53 +1.1 228,302 + RR42_RS21840 0.53 -0.1 228,302 + RR42_RS21840 0.53 -0.8 228,311 + RR42_RS21840 0.56 +0.5 228,311 + RR42_RS21840 0.56 -0.4 228,312 - RR42_RS21840 0.56 -0.2 228,312 - RR42_RS21840 0.56 -1.2 228,312 - RR42_RS21840 0.56 -0.2 228,380 + RR42_RS21840 0.81 +3.2 228,380 + RR42_RS21840 0.81 -0.0 228,381 - RR42_RS21840 0.81 -3.0 228,432 - -0.4 228,554 - +0.4 228,633 + -0.5 228,633 + -0.7 228,634 - +0.5 228,880 - RR42_RS21845 0.34 -0.1 228,930 - RR42_RS21845 0.39 -0.5 229,014 + RR42_RS21845 0.48 -1.5 229,014 + RR42_RS21845 0.48 -0.7 229,014 + RR42_RS21845 0.48 -0.5 229,014 + RR42_RS21845 0.48 +0.5 229,015 - RR42_RS21845 0.48 -0.1 229,020 + RR42_RS21845 0.48 -0.2 229,020 + RR42_RS21845 0.48 -0.8 229,020 + RR42_RS21845 0.48 +0.1 229,020 + RR42_RS21845 0.48 -0.6 229,020 + RR42_RS21845 0.48 -0.3 229,020 + RR42_RS21845 0.48 +0.7 229,021 - RR42_RS21845 0.48 +0.6 229,021 - RR42_RS21845 0.48 -0.9 229,021 - RR42_RS21845 0.48 -0.9 229,021 - RR42_RS21845 0.48 -0.0 229,021 - RR42_RS21845 0.48 +0.8 229,021 - RR42_RS21845 0.48 -0.2 229,108 - RR42_RS21845 0.57 -0.4
Or see this region's nucleotide sequence