Experiment: R2A with A22 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS21760 and RR42_RS21765 are separated by 139 nucleotides RR42_RS21765 and RR42_RS21770 are separated by 14 nucleotides
RR42_RS21760: RR42_RS21760 - succinate-semialdehyde dehydrogenase, at 210,422 to 211,870
_RS21760
RR42_RS21765: RR42_RS21765 - 3-oxoacyl-ACP reductase, at 212,010 to 212,750
_RS21765
RR42_RS21770: RR42_RS21770 - dihydrolipoamide dehydrogenase, at 212,765 to 214,165
_RS21770
Position (kb) 212
213 Strain fitness (log2 ratio) -1
0
1
2
3 at 211.793 kb on - strand at 211.825 kb on + strand at 211.827 kb on + strand at 211.890 kb on - strand at 211.934 kb on - strand at 212.024 kb on - strand at 212.024 kb on - strand at 212.212 kb on - strand, within RR42_RS21765 at 212.306 kb on - strand, within RR42_RS21765 at 212.392 kb on + strand, within RR42_RS21765 at 212.471 kb on - strand, within RR42_RS21765 at 212.746 kb on + strand at 212.747 kb on - strand at 212.747 kb on - strand at 212.770 kb on + strand at 212.771 kb on - strand at 212.771 kb on - strand at 212.893 kb on - strand at 212.930 kb on - strand, within RR42_RS21770 at 213.111 kb on + strand, within RR42_RS21770 at 213.112 kb on - strand, within RR42_RS21770 at 213.112 kb on - strand, within RR42_RS21770 at 213.243 kb on + strand, within RR42_RS21770 at 213.243 kb on + strand, within RR42_RS21770 at 213.244 kb on - strand, within RR42_RS21770 at 213.286 kb on + strand, within RR42_RS21770 at 213.287 kb on - strand, within RR42_RS21770
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.01 mg/ml remove 211,793 - -0.4 211,825 + +0.9 211,827 + +2.9 211,890 - -1.0 211,934 - +0.7 212,024 - -0.1 212,024 - -0.0 212,212 - RR42_RS21765 0.27 -0.3 212,306 - RR42_RS21765 0.40 +1.0 212,392 + RR42_RS21765 0.52 +0.4 212,471 - RR42_RS21765 0.62 +1.7 212,746 + -0.5 212,747 - -0.7 212,747 - +0.1 212,770 + -0.4 212,771 - -0.2 212,771 - -0.6 212,893 - -1.7 212,930 - RR42_RS21770 0.12 -0.2 213,111 + RR42_RS21770 0.25 -0.4 213,112 - RR42_RS21770 0.25 -0.6 213,112 - RR42_RS21770 0.25 +0.1 213,243 + RR42_RS21770 0.34 -0.8 213,243 + RR42_RS21770 0.34 -0.4 213,244 - RR42_RS21770 0.34 -1.0 213,286 + RR42_RS21770 0.37 -1.3 213,287 - RR42_RS21770 0.37 +0.8
Or see this region's nucleotide sequence