Experiment: R2A with A22 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS21600 and RR42_RS21605 overlap by 20 nucleotides RR42_RS21605 and RR42_RS21610 are separated by 443 nucleotides
RR42_RS21600: RR42_RS21600 - inorganic pyrophosphatase/exopolyphosphatase, at 173,971 to 174,999
_RS21600
RR42_RS21605: RR42_RS21605 - S-adenosylhomocysteine hydrolase, at 174,980 to 175,387
_RS21605
RR42_RS21610: RR42_RS21610 - MFS transporter permease, at 175,831 to 177,141
_RS21610
Position (kb) 174
175
176 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 174.166 kb on + strand, within RR42_RS21600 at 174.166 kb on + strand, within RR42_RS21600 at 174.242 kb on + strand, within RR42_RS21600 at 174.242 kb on + strand, within RR42_RS21600 at 174.243 kb on - strand, within RR42_RS21600 at 174.414 kb on - strand, within RR42_RS21600 at 174.518 kb on + strand, within RR42_RS21600 at 174.519 kb on - strand, within RR42_RS21600 at 174.536 kb on + strand, within RR42_RS21600 at 174.540 kb on - strand, within RR42_RS21600 at 174.680 kb on + strand, within RR42_RS21600 at 174.681 kb on - strand, within RR42_RS21600 at 174.803 kb on - strand, within RR42_RS21600 at 174.819 kb on + strand, within RR42_RS21600 at 174.820 kb on - strand, within RR42_RS21600 at 174.999 kb on + strand at 175.101 kb on + strand, within RR42_RS21605 at 175.101 kb on + strand, within RR42_RS21605 at 175.101 kb on + strand, within RR42_RS21605 at 175.101 kb on + strand, within RR42_RS21605 at 175.418 kb on + strand at 175.418 kb on + strand at 175.427 kb on + strand at 175.428 kb on - strand at 175.593 kb on + strand at 175.593 kb on + strand at 175.593 kb on + strand at 175.593 kb on + strand at 175.594 kb on - strand at 175.594 kb on - strand at 175.594 kb on - strand at 175.594 kb on - strand at 175.594 kb on - strand at 175.705 kb on + strand at 175.705 kb on + strand at 175.706 kb on - strand at 175.706 kb on - strand at 175.706 kb on - strand at 175.709 kb on + strand at 175.709 kb on + strand at 175.710 kb on - strand at 175.956 kb on - strand at 176.080 kb on + strand, within RR42_RS21610 at 176.080 kb on + strand, within RR42_RS21610 at 176.081 kb on - strand, within RR42_RS21610 at 176.081 kb on - strand, within RR42_RS21610 at 176.081 kb on - strand, within RR42_RS21610 at 176.339 kb on + strand, within RR42_RS21610 at 176.339 kb on + strand, within RR42_RS21610
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.01 mg/ml remove 174,166 + RR42_RS21600 0.19 +0.7 174,166 + RR42_RS21600 0.19 +0.3 174,242 + RR42_RS21600 0.26 +0.8 174,242 + RR42_RS21600 0.26 +1.2 174,243 - RR42_RS21600 0.26 +0.3 174,414 - RR42_RS21600 0.43 +0.2 174,518 + RR42_RS21600 0.53 -0.4 174,519 - RR42_RS21600 0.53 -0.1 174,536 + RR42_RS21600 0.55 -0.4 174,540 - RR42_RS21600 0.55 -1.5 174,680 + RR42_RS21600 0.69 -0.8 174,681 - RR42_RS21600 0.69 +0.4 174,803 - RR42_RS21600 0.81 -0.2 174,819 + RR42_RS21600 0.82 -0.8 174,820 - RR42_RS21600 0.83 +0.4 174,999 + -0.1 175,101 + RR42_RS21605 0.30 -1.9 175,101 + RR42_RS21605 0.30 +0.6 175,101 + RR42_RS21605 0.30 -2.9 175,101 + RR42_RS21605 0.30 +0.2 175,418 + -1.8 175,418 + -2.3 175,427 + +0.5 175,428 - -1.7 175,593 + -0.4 175,593 + -0.5 175,593 + -0.3 175,593 + -0.7 175,594 - +0.0 175,594 - +1.5 175,594 - +0.9 175,594 - +0.1 175,594 - +0.5 175,705 + -0.3 175,705 + -0.4 175,706 - +0.2 175,706 - -0.2 175,706 - -0.4 175,709 + +0.7 175,709 + +2.9 175,710 - +0.0 175,956 - +0.8 176,080 + RR42_RS21610 0.19 +1.7 176,080 + RR42_RS21610 0.19 -0.3 176,081 - RR42_RS21610 0.19 -1.0 176,081 - RR42_RS21610 0.19 -0.5 176,081 - RR42_RS21610 0.19 +0.3 176,339 + RR42_RS21610 0.39 -0.3 176,339 + RR42_RS21610 0.39 -0.1
Or see this region's nucleotide sequence