Experiment: R2A with A22 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS21570 and RR42_RS21575 are separated by 125 nucleotides RR42_RS21575 and RR42_RS21580 are separated by 34 nucleotides RR42_RS21580 and RR42_RS21585 are separated by 45 nucleotides
RR42_RS21570: RR42_RS21570 - membrane protein, at 164,956 to 166,104
_RS21570
RR42_RS21575: RR42_RS21575 - peptidase E, at 166,230 to 166,985
_RS21575
RR42_RS21580: RR42_RS21580 - cysteine desulfurase, at 167,020 to 168,204
_RS21580
RR42_RS21585: RR42_RS21585 - membrane protein, at 168,250 to 169,857
_RS21585
Position (kb) 167
168
169 Strain fitness (log2 ratio) -2
-1
0
1 at 166.128 kb on - strand at 166.128 kb on - strand at 166.128 kb on - strand at 166.318 kb on - strand, within RR42_RS21575 at 166.631 kb on - strand, within RR42_RS21575 at 166.831 kb on + strand, within RR42_RS21575 at 166.831 kb on + strand, within RR42_RS21575 at 166.831 kb on + strand, within RR42_RS21575 at 166.831 kb on + strand, within RR42_RS21575 at 166.831 kb on + strand, within RR42_RS21575 at 166.831 kb on + strand, within RR42_RS21575 at 166.831 kb on + strand, within RR42_RS21575 at 166.832 kb on - strand, within RR42_RS21575 at 166.832 kb on - strand, within RR42_RS21575 at 166.832 kb on - strand, within RR42_RS21575 at 166.832 kb on - strand, within RR42_RS21575 at 166.832 kb on - strand, within RR42_RS21575 at 166.934 kb on - strand at 166.998 kb on + strand at 167.073 kb on + strand at 167.074 kb on - strand at 167.074 kb on - strand at 167.081 kb on + strand at 167.082 kb on - strand at 167.117 kb on + strand at 167.264 kb on + strand, within RR42_RS21580 at 167.468 kb on + strand, within RR42_RS21580 at 167.468 kb on + strand, within RR42_RS21580 at 167.469 kb on - strand, within RR42_RS21580 at 167.469 kb on - strand, within RR42_RS21580 at 167.531 kb on + strand, within RR42_RS21580 at 167.943 kb on + strand, within RR42_RS21580 at 167.943 kb on + strand, within RR42_RS21580 at 167.943 kb on + strand, within RR42_RS21580 at 167.944 kb on - strand, within RR42_RS21580 at 167.944 kb on - strand, within RR42_RS21580 at 168.035 kb on + strand, within RR42_RS21580 at 168.035 kb on + strand, within RR42_RS21580 at 168.036 kb on - strand, within RR42_RS21580 at 168.036 kb on - strand, within RR42_RS21580 at 168.036 kb on - strand, within RR42_RS21580 at 168.062 kb on + strand, within RR42_RS21580 at 168.152 kb on + strand at 168.153 kb on - strand at 168.153 kb on - strand at 168.153 kb on - strand at 168.153 kb on - strand at 168.251 kb on + strand at 168.251 kb on + strand at 168.251 kb on + strand at 168.251 kb on + strand at 168.251 kb on + strand at 168.251 kb on + strand at 168.394 kb on + strand at 168.561 kb on - strand, within RR42_RS21585 at 168.722 kb on + strand, within RR42_RS21585 at 168.740 kb on + strand, within RR42_RS21585 at 168.915 kb on - strand, within RR42_RS21585 at 168.932 kb on + strand, within RR42_RS21585 at 168.966 kb on + strand, within RR42_RS21585 at 168.967 kb on - strand, within RR42_RS21585 at 169.017 kb on - strand, within RR42_RS21585 at 169.173 kb on + strand, within RR42_RS21585 at 169.175 kb on + strand, within RR42_RS21585 at 169.176 kb on - strand, within RR42_RS21585 at 169.176 kb on - strand, within RR42_RS21585 at 169.176 kb on - strand, within RR42_RS21585 at 169.176 kb on - strand, within RR42_RS21585 at 169.176 kb on - strand, within RR42_RS21585
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.01 mg/ml remove 166,128 - -0.3 166,128 - +0.7 166,128 - -0.4 166,318 - RR42_RS21575 0.12 -0.3 166,631 - RR42_RS21575 0.53 +0.3 166,831 + RR42_RS21575 0.79 +1.5 166,831 + RR42_RS21575 0.79 -1.0 166,831 + RR42_RS21575 0.79 +1.1 166,831 + RR42_RS21575 0.79 -0.8 166,831 + RR42_RS21575 0.79 +0.2 166,831 + RR42_RS21575 0.79 +0.1 166,831 + RR42_RS21575 0.79 +0.1 166,832 - RR42_RS21575 0.80 +0.9 166,832 - RR42_RS21575 0.80 +0.0 166,832 - RR42_RS21575 0.80 +0.9 166,832 - RR42_RS21575 0.80 -0.8 166,832 - RR42_RS21575 0.80 +0.5 166,934 - +0.7 166,998 + -0.0 167,073 + +0.0 167,074 - -0.8 167,074 - -0.4 167,081 + -2.2 167,082 - +1.4 167,117 + -1.0 167,264 + RR42_RS21580 0.21 +0.3 167,468 + RR42_RS21580 0.38 +1.2 167,468 + RR42_RS21580 0.38 +1.7 167,469 - RR42_RS21580 0.38 +0.6 167,469 - RR42_RS21580 0.38 +0.1 167,531 + RR42_RS21580 0.43 -0.6 167,943 + RR42_RS21580 0.78 -1.4 167,943 + RR42_RS21580 0.78 +0.8 167,943 + RR42_RS21580 0.78 +0.1 167,944 - RR42_RS21580 0.78 +0.2 167,944 - RR42_RS21580 0.78 -1.2 168,035 + RR42_RS21580 0.86 -0.4 168,035 + RR42_RS21580 0.86 +0.4 168,036 - RR42_RS21580 0.86 -0.1 168,036 - RR42_RS21580 0.86 +0.8 168,036 - RR42_RS21580 0.86 +0.5 168,062 + RR42_RS21580 0.88 -0.8 168,152 + -0.2 168,153 - +0.8 168,153 - +0.4 168,153 - +1.2 168,153 - -0.4 168,251 + +0.3 168,251 + +1.2 168,251 + +0.3 168,251 + +0.6 168,251 + -0.4 168,251 + -1.1 168,394 + +0.7 168,561 - RR42_RS21585 0.19 -1.0 168,722 + RR42_RS21585 0.29 -0.2 168,740 + RR42_RS21585 0.30 -0.6 168,915 - RR42_RS21585 0.41 -0.2 168,932 + RR42_RS21585 0.42 -1.1 168,966 + RR42_RS21585 0.45 +1.2 168,967 - RR42_RS21585 0.45 +0.6 169,017 - RR42_RS21585 0.48 +0.2 169,173 + RR42_RS21585 0.57 +0.7 169,175 + RR42_RS21585 0.58 -2.2 169,176 - RR42_RS21585 0.58 -0.3 169,176 - RR42_RS21585 0.58 +0.2 169,176 - RR42_RS21585 0.58 +0.2 169,176 - RR42_RS21585 0.58 +1.0 169,176 - RR42_RS21585 0.58 -0.1
Or see this region's nucleotide sequence