Experiment: R2A with A22 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS21375 and RR42_RS21380 are separated by 40 nucleotides RR42_RS21380 and RR42_RS21385 are separated by 18 nucleotides
RR42_RS21375: RR42_RS21375 - succinate-semialdehyde dehydrogenase, at 125,317 to 126,783
_RS21375
RR42_RS21380: RR42_RS21380 - cupin, at 126,824 to 127,141
_RS21380
RR42_RS21385: RR42_RS21385 - acetylpolyamine aminohydrolase, at 127,160 to 128,233
_RS21385
Position (kb) 126
127
128 Strain fitness (log2 ratio) -1
0
1 at 126.212 kb on - strand, within RR42_RS21375 at 126.229 kb on + strand, within RR42_RS21375 at 126.229 kb on + strand, within RR42_RS21375 at 126.740 kb on - strand at 126.755 kb on - strand at 126.781 kb on + strand at 126.799 kb on + strand at 126.938 kb on + strand, within RR42_RS21380 at 126.938 kb on + strand, within RR42_RS21380 at 127.451 kb on + strand, within RR42_RS21385 at 127.454 kb on + strand, within RR42_RS21385 at 127.454 kb on + strand, within RR42_RS21385 at 127.455 kb on - strand, within RR42_RS21385 at 127.455 kb on - strand, within RR42_RS21385 at 127.455 kb on - strand, within RR42_RS21385 at 127.526 kb on + strand, within RR42_RS21385 at 127.527 kb on - strand, within RR42_RS21385 at 127.527 kb on - strand, within RR42_RS21385 at 127.650 kb on - strand, within RR42_RS21385 at 127.880 kb on + strand, within RR42_RS21385 at 127.880 kb on + strand, within RR42_RS21385 at 127.881 kb on - strand, within RR42_RS21385 at 127.881 kb on - strand, within RR42_RS21385 at 127.926 kb on - strand, within RR42_RS21385 at 127.926 kb on - strand, within RR42_RS21385 at 127.926 kb on - strand, within RR42_RS21385 at 128.012 kb on + strand, within RR42_RS21385 at 128.055 kb on - strand, within RR42_RS21385 at 128.055 kb on - strand, within RR42_RS21385 at 128.055 kb on - strand, within RR42_RS21385 at 128.055 kb on - strand, within RR42_RS21385 at 128.055 kb on - strand, within RR42_RS21385
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.01 mg/ml remove 126,212 - RR42_RS21375 0.61 +0.3 126,229 + RR42_RS21375 0.62 -1.1 126,229 + RR42_RS21375 0.62 -0.3 126,740 - +0.9 126,755 - -0.6 126,781 + -0.4 126,799 + +0.1 126,938 + RR42_RS21380 0.36 -0.8 126,938 + RR42_RS21380 0.36 -1.1 127,451 + RR42_RS21385 0.27 +0.9 127,454 + RR42_RS21385 0.27 +0.5 127,454 + RR42_RS21385 0.27 -0.4 127,455 - RR42_RS21385 0.27 -0.2 127,455 - RR42_RS21385 0.27 -0.5 127,455 - RR42_RS21385 0.27 -0.4 127,526 + RR42_RS21385 0.34 -1.4 127,527 - RR42_RS21385 0.34 -0.4 127,527 - RR42_RS21385 0.34 +0.6 127,650 - RR42_RS21385 0.46 +0.2 127,880 + RR42_RS21385 0.67 +0.0 127,880 + RR42_RS21385 0.67 +0.1 127,881 - RR42_RS21385 0.67 +1.4 127,881 - RR42_RS21385 0.67 +0.4 127,926 - RR42_RS21385 0.71 -1.0 127,926 - RR42_RS21385 0.71 +1.2 127,926 - RR42_RS21385 0.71 -0.2 128,012 + RR42_RS21385 0.79 -0.4 128,055 - RR42_RS21385 0.83 +0.5 128,055 - RR42_RS21385 0.83 -0.6 128,055 - RR42_RS21385 0.83 +0.5 128,055 - RR42_RS21385 0.83 -0.5 128,055 - RR42_RS21385 0.83 +0.1
Or see this region's nucleotide sequence