Experiment: R2A with A22 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS21210 and RR42_RS21215 are separated by 230 nucleotides RR42_RS21215 and RR42_RS21220 are separated by 18 nucleotides RR42_RS21220 and RR42_RS21225 are separated by 19 nucleotides RR42_RS21225 and RR42_RS21230 are separated by 513 nucleotides
RR42_RS21210: RR42_RS21210 - hypothetical protein, at 83,457 to 84,767
_RS21210
RR42_RS21215: RR42_RS21215 - membrane protein, at 84,998 to 85,318
_RS21215
RR42_RS21220: RR42_RS21220 - iron transporter FeoB, at 85,337 to 87,208
_RS21220
RR42_RS21225: RR42_RS21225 - iron transporter FeoA, at 87,228 to 87,533
_RS21225
RR42_RS21230: RR42_RS21230 - ABC transporter substrate-binding protein, at 88,047 to 89,039
_RS21230
Position (kb) 85
86
87
88 Strain fitness (log2 ratio) -1
0
1
2 at 84.433 kb on + strand, within RR42_RS21210 at 84.434 kb on - strand, within RR42_RS21210 at 84.492 kb on + strand, within RR42_RS21210 at 84.492 kb on + strand, within RR42_RS21210 at 84.492 kb on + strand, within RR42_RS21210 at 84.493 kb on - strand, within RR42_RS21210 at 84.493 kb on - strand, within RR42_RS21210 at 84.493 kb on - strand, within RR42_RS21210 at 84.586 kb on - strand, within RR42_RS21210 at 84.629 kb on - strand, within RR42_RS21210 at 85.984 kb on - strand, within RR42_RS21220 at 86.067 kb on + strand, within RR42_RS21220 at 86.992 kb on - strand, within RR42_RS21220 at 87.079 kb on - strand at 87.209 kb on - strand at 87.209 kb on - strand at 87.209 kb on - strand at 87.597 kb on - strand at 87.619 kb on - strand at 87.989 kb on - strand at 88.048 kb on + strand at 88.049 kb on - strand at 88.049 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.01 mg/ml remove 84,433 + RR42_RS21210 0.74 +0.1 84,434 - RR42_RS21210 0.75 -0.5 84,492 + RR42_RS21210 0.79 +0.8 84,492 + RR42_RS21210 0.79 +0.3 84,492 + RR42_RS21210 0.79 +0.3 84,493 - RR42_RS21210 0.79 -0.1 84,493 - RR42_RS21210 0.79 -0.5 84,493 - RR42_RS21210 0.79 -0.5 84,586 - RR42_RS21210 0.86 +0.3 84,629 - RR42_RS21210 0.89 -0.1 85,984 - RR42_RS21220 0.35 +2.2 86,067 + RR42_RS21220 0.39 -0.9 86,992 - RR42_RS21220 0.88 -0.4 87,079 - -1.7 87,209 - -1.0 87,209 - -0.4 87,209 - -0.2 87,597 - -0.1 87,619 - -0.2 87,989 - +1.0 88,048 + -0.2 88,049 - +1.2 88,049 - +0.3
Or see this region's nucleotide sequence