Experiment: R2A with A22 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS21075 and RR42_RS21080 are separated by 40 nucleotides RR42_RS21080 and RR42_RS21085 overlap by 4 nucleotides
RR42_RS21075: RR42_RS21075 - phosphoribosyltransferase, at 54,061 to 54,768
_RS21075
RR42_RS21080: RR42_RS21080 - 3-isopropylmalate dehydratase, at 54,809 to 55,321
_RS21080
RR42_RS21085: RR42_RS21085 - 3-isopropylmalate dehydratase, at 55,318 to 56,625
_RS21085
Position (kb) 54
55
56 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 53.947 kb on + strand at 53.947 kb on - strand at 53.948 kb on - strand at 53.949 kb on + strand at 53.949 kb on + strand at 53.949 kb on + strand at 53.950 kb on - strand at 53.950 kb on - strand at 53.950 kb on - strand at 53.950 kb on - strand at 54.691 kb on + strand, within RR42_RS21075 at 55.126 kb on + strand, within RR42_RS21080 at 55.228 kb on + strand, within RR42_RS21080 at 55.229 kb on - strand, within RR42_RS21080 at 55.229 kb on - strand, within RR42_RS21080 at 55.229 kb on - strand, within RR42_RS21080 at 55.229 kb on - strand, within RR42_RS21080 at 55.606 kb on + strand, within RR42_RS21085 at 55.606 kb on + strand, within RR42_RS21085 at 55.606 kb on + strand, within RR42_RS21085 at 55.606 kb on + strand, within RR42_RS21085 at 55.607 kb on - strand, within RR42_RS21085 at 55.607 kb on - strand, within RR42_RS21085 at 55.607 kb on - strand, within RR42_RS21085 at 55.697 kb on + strand, within RR42_RS21085 at 55.697 kb on + strand, within RR42_RS21085 at 56.012 kb on + strand, within RR42_RS21085 at 56.012 kb on + strand, within RR42_RS21085 at 56.012 kb on + strand, within RR42_RS21085 at 56.013 kb on - strand, within RR42_RS21085 at 56.027 kb on + strand, within RR42_RS21085 at 56.028 kb on - strand, within RR42_RS21085 at 56.127 kb on + strand, within RR42_RS21085 at 56.127 kb on + strand, within RR42_RS21085 at 56.127 kb on + strand, within RR42_RS21085 at 56.127 kb on + strand, within RR42_RS21085 at 56.127 kb on + strand, within RR42_RS21085 at 56.127 kb on + strand, within RR42_RS21085 at 56.127 kb on + strand, within RR42_RS21085 at 56.128 kb on - strand, within RR42_RS21085 at 56.199 kb on + strand, within RR42_RS21085 at 56.199 kb on + strand, within RR42_RS21085 at 56.199 kb on + strand, within RR42_RS21085 at 56.200 kb on - strand, within RR42_RS21085 at 56.200 kb on - strand, within RR42_RS21085 at 56.201 kb on + strand, within RR42_RS21085 at 56.201 kb on + strand, within RR42_RS21085 at 56.202 kb on - strand, within RR42_RS21085 at 56.202 kb on - strand, within RR42_RS21085 at 56.287 kb on + strand, within RR42_RS21085 at 56.287 kb on + strand, within RR42_RS21085 at 56.288 kb on - strand, within RR42_RS21085
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.01 mg/ml remove 53,947 + +0.4 53,947 - +0.0 53,948 - +0.4 53,949 + +0.2 53,949 + -1.7 53,949 + -0.1 53,950 - +0.9 53,950 - -1.0 53,950 - +0.2 53,950 - -0.2 54,691 + RR42_RS21075 0.89 -1.5 55,126 + RR42_RS21080 0.62 -0.4 55,228 + RR42_RS21080 0.82 -2.4 55,229 - RR42_RS21080 0.82 -0.6 55,229 - RR42_RS21080 0.82 -1.5 55,229 - RR42_RS21080 0.82 -0.8 55,229 - RR42_RS21080 0.82 -1.1 55,606 + RR42_RS21085 0.22 +1.7 55,606 + RR42_RS21085 0.22 +0.7 55,606 + RR42_RS21085 0.22 -1.0 55,606 + RR42_RS21085 0.22 -0.3 55,607 - RR42_RS21085 0.22 +0.3 55,607 - RR42_RS21085 0.22 +0.7 55,607 - RR42_RS21085 0.22 -2.2 55,697 + RR42_RS21085 0.29 -1.4 55,697 + RR42_RS21085 0.29 +0.6 56,012 + RR42_RS21085 0.53 -0.5 56,012 + RR42_RS21085 0.53 +0.3 56,012 + RR42_RS21085 0.53 +1.2 56,013 - RR42_RS21085 0.53 +1.6 56,027 + RR42_RS21085 0.54 -0.6 56,028 - RR42_RS21085 0.54 -1.2 56,127 + RR42_RS21085 0.62 -0.3 56,127 + RR42_RS21085 0.62 +0.1 56,127 + RR42_RS21085 0.62 +0.7 56,127 + RR42_RS21085 0.62 +0.3 56,127 + RR42_RS21085 0.62 +1.2 56,127 + RR42_RS21085 0.62 +0.1 56,127 + RR42_RS21085 0.62 -0.8 56,128 - RR42_RS21085 0.62 +0.2 56,199 + RR42_RS21085 0.67 -0.0 56,199 + RR42_RS21085 0.67 -2.8 56,199 + RR42_RS21085 0.67 -0.4 56,200 - RR42_RS21085 0.67 -0.2 56,200 - RR42_RS21085 0.67 -0.3 56,201 + RR42_RS21085 0.68 -0.6 56,201 + RR42_RS21085 0.68 -1.0 56,202 - RR42_RS21085 0.68 -0.7 56,202 - RR42_RS21085 0.68 -0.7 56,287 + RR42_RS21085 0.74 -0.3 56,287 + RR42_RS21085 0.74 -0.1 56,288 - RR42_RS21085 0.74 +0.3
Or see this region's nucleotide sequence