Experiment: R2A with A22 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS21070 and RR42_RS21075 are separated by 409 nucleotides RR42_RS21075 and RR42_RS21080 are separated by 40 nucleotides RR42_RS21080 and RR42_RS21085 overlap by 4 nucleotides
RR42_RS21070: RR42_RS21070 - hypothetical protein, at 53,469 to 53,651
_RS21070
RR42_RS21075: RR42_RS21075 - phosphoribosyltransferase, at 54,061 to 54,768
_RS21075
RR42_RS21080: RR42_RS21080 - 3-isopropylmalate dehydratase, at 54,809 to 55,321
_RS21080
RR42_RS21085: RR42_RS21085 - 3-isopropylmalate dehydratase, at 55,318 to 56,625
_RS21085
Position (kb) 54
55 Strain fitness (log2 ratio) -2
-1
0
1 at 53.100 kb on - strand at 53.172 kb on + strand at 53.207 kb on + strand at 53.207 kb on + strand at 53.208 kb on - strand at 53.208 kb on - strand at 53.249 kb on + strand at 53.332 kb on - strand at 53.470 kb on + strand at 53.471 kb on - strand at 53.489 kb on - strand, within RR42_RS21070 at 53.489 kb on - strand, within RR42_RS21070 at 53.522 kb on + strand, within RR42_RS21070 at 53.523 kb on - strand, within RR42_RS21070 at 53.523 kb on - strand, within RR42_RS21070 at 53.580 kb on + strand, within RR42_RS21070 at 53.581 kb on - strand, within RR42_RS21070 at 53.581 kb on - strand, within RR42_RS21070 at 53.645 kb on + strand at 53.645 kb on + strand at 53.646 kb on - strand at 53.646 kb on - strand at 53.646 kb on - strand at 53.776 kb on - strand at 53.794 kb on + strand at 53.794 kb on + strand at 53.795 kb on - strand at 53.795 kb on - strand at 53.795 kb on - strand at 53.947 kb on + strand at 53.947 kb on - strand at 53.948 kb on - strand at 53.949 kb on + strand at 53.949 kb on + strand at 53.949 kb on + strand at 53.950 kb on - strand at 53.950 kb on - strand at 53.950 kb on - strand at 53.950 kb on - strand at 54.691 kb on + strand, within RR42_RS21075 at 55.126 kb on + strand, within RR42_RS21080 at 55.228 kb on + strand, within RR42_RS21080 at 55.229 kb on - strand, within RR42_RS21080 at 55.229 kb on - strand, within RR42_RS21080 at 55.229 kb on - strand, within RR42_RS21080 at 55.229 kb on - strand, within RR42_RS21080 at 55.606 kb on + strand, within RR42_RS21085 at 55.606 kb on + strand, within RR42_RS21085 at 55.606 kb on + strand, within RR42_RS21085 at 55.606 kb on + strand, within RR42_RS21085 at 55.607 kb on - strand, within RR42_RS21085 at 55.607 kb on - strand, within RR42_RS21085 at 55.607 kb on - strand, within RR42_RS21085 at 55.697 kb on + strand, within RR42_RS21085 at 55.697 kb on + strand, within RR42_RS21085
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.01 mg/ml remove 53,100 - +1.2 53,172 + +1.1 53,207 + -0.6 53,207 + +0.3 53,208 - +1.2 53,208 - -1.6 53,249 + -0.1 53,332 - -0.8 53,470 + +0.0 53,471 - +0.2 53,489 - RR42_RS21070 0.11 +0.0 53,489 - RR42_RS21070 0.11 -1.0 53,522 + RR42_RS21070 0.29 +0.9 53,523 - RR42_RS21070 0.30 -0.2 53,523 - RR42_RS21070 0.30 +0.8 53,580 + RR42_RS21070 0.61 -0.1 53,581 - RR42_RS21070 0.61 -0.5 53,581 - RR42_RS21070 0.61 -0.1 53,645 + +0.2 53,645 + -1.2 53,646 - -0.1 53,646 - +0.6 53,646 - +0.5 53,776 - +0.6 53,794 + +0.2 53,794 + -0.8 53,795 - -1.4 53,795 - +1.0 53,795 - -0.6 53,947 + +0.4 53,947 - +0.0 53,948 - +0.4 53,949 + +0.2 53,949 + -1.7 53,949 + -0.1 53,950 - +0.9 53,950 - -1.0 53,950 - +0.2 53,950 - -0.2 54,691 + RR42_RS21075 0.89 -1.5 55,126 + RR42_RS21080 0.62 -0.4 55,228 + RR42_RS21080 0.82 -2.4 55,229 - RR42_RS21080 0.82 -0.6 55,229 - RR42_RS21080 0.82 -1.5 55,229 - RR42_RS21080 0.82 -0.8 55,229 - RR42_RS21080 0.82 -1.1 55,606 + RR42_RS21085 0.22 +1.7 55,606 + RR42_RS21085 0.22 +0.7 55,606 + RR42_RS21085 0.22 -1.0 55,606 + RR42_RS21085 0.22 -0.3 55,607 - RR42_RS21085 0.22 +0.3 55,607 - RR42_RS21085 0.22 +0.7 55,607 - RR42_RS21085 0.22 -2.2 55,697 + RR42_RS21085 0.29 -1.4 55,697 + RR42_RS21085 0.29 +0.6
Or see this region's nucleotide sequence