Experiment: R2A with A22 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS20565 and RR42_RS20570 are separated by 6 nucleotides RR42_RS20570 and RR42_RS20575 overlap by 4 nucleotides RR42_RS20575 and RR42_RS20580 are separated by 129 nucleotides RR42_RS20580 and RR42_RS20585 are separated by 14 nucleotides
RR42_RS20565: RR42_RS20565 - hypothetical protein, at 4,461,383 to 4,465,420
_RS20565
RR42_RS20570: RR42_RS20570 - biopolymer transporter ExbD, at 4,465,427 to 4,465,936
_RS20570
RR42_RS20575: RR42_RS20575 - hypothetical protein, at 4,465,933 to 4,466,253
_RS20575
RR42_RS20580: RR42_RS20580 - lipoprotein, at 4,466,383 to 4,467,204
_RS20580
RR42_RS20585: RR42_RS20585 - pyridine nucleotide-disulfide oxidoreductase, at 4,467,219 to 4,468,670
_RS20585
Position (kb)
4465
4466
4467 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4465.435 kb on + strand at 4465.435 kb on + strand at 4465.457 kb on + strand at 4465.458 kb on - strand at 4465.458 kb on - strand at 4465.517 kb on + strand, within RR42_RS20570 at 4465.596 kb on - strand, within RR42_RS20570 at 4466.058 kb on + strand, within RR42_RS20575 at 4466.197 kb on + strand, within RR42_RS20575 at 4466.224 kb on + strand at 4466.224 kb on + strand at 4466.224 kb on + strand at 4466.224 kb on + strand at 4466.225 kb on - strand at 4466.225 kb on - strand at 4466.225 kb on - strand at 4466.251 kb on + strand at 4466.262 kb on - strand at 4466.264 kb on + strand at 4466.264 kb on + strand at 4466.265 kb on - strand at 4466.296 kb on + strand at 4466.297 kb on - strand at 4466.300 kb on + strand at 4466.467 kb on + strand, within RR42_RS20580 at 4466.467 kb on + strand, within RR42_RS20580 at 4466.467 kb on + strand, within RR42_RS20580 at 4466.467 kb on + strand, within RR42_RS20580 at 4466.468 kb on - strand, within RR42_RS20580 at 4466.468 kb on - strand, within RR42_RS20580 at 4466.641 kb on + strand, within RR42_RS20580 at 4466.641 kb on + strand, within RR42_RS20580 at 4466.687 kb on - strand, within RR42_RS20580 at 4466.716 kb on + strand, within RR42_RS20580 at 4467.071 kb on - strand, within RR42_RS20580 at 4467.071 kb on - strand, within RR42_RS20580 at 4467.118 kb on + strand, within RR42_RS20580 at 4467.119 kb on - strand, within RR42_RS20580
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.01 mg/ml remove 4,465,435 + -0.5 4,465,435 + -0.6 4,465,457 + -1.8 4,465,458 - -0.5 4,465,458 - -1.9 4,465,517 + RR42_RS20570 0.18 +0.5 4,465,596 - RR42_RS20570 0.33 -0.1 4,466,058 + RR42_RS20575 0.39 +0.7 4,466,197 + RR42_RS20575 0.82 +0.4 4,466,224 + +0.7 4,466,224 + -0.0 4,466,224 + +0.6 4,466,224 + -0.5 4,466,225 - -0.5 4,466,225 - -1.3 4,466,225 - +0.4 4,466,251 + +0.0 4,466,262 - +0.4 4,466,264 + -0.2 4,466,264 + +0.3 4,466,265 - +0.9 4,466,296 + -0.1 4,466,297 - +1.0 4,466,300 + -1.1 4,466,467 + RR42_RS20580 0.10 -0.5 4,466,467 + RR42_RS20580 0.10 -0.3 4,466,467 + RR42_RS20580 0.10 +1.0 4,466,467 + RR42_RS20580 0.10 +0.7 4,466,468 - RR42_RS20580 0.10 -0.6 4,466,468 - RR42_RS20580 0.10 -0.2 4,466,641 + RR42_RS20580 0.31 +0.3 4,466,641 + RR42_RS20580 0.31 +0.5 4,466,687 - RR42_RS20580 0.37 +2.4 4,466,716 + RR42_RS20580 0.41 -0.5 4,467,071 - RR42_RS20580 0.84 -0.2 4,467,071 - RR42_RS20580 0.84 -0.2 4,467,118 + RR42_RS20580 0.89 -0.1 4,467,119 - RR42_RS20580 0.90 -0.2
Or see this region's nucleotide sequence