Experiment: R2A with A22 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS20430 and RR42_RS20435 are separated by 60 nucleotides RR42_RS20435 and RR42_RS20440 are separated by 52 nucleotides RR42_RS20440 and RR42_RS20445 overlap by 4 nucleotides RR42_RS20445 and RR42_RS20450 are separated by 175 nucleotides
RR42_RS20430: RR42_RS20430 - tRNA-Phe, at 4,425,921 to 4,425,996
_RS20430
RR42_RS20435: RR42_RS20435 - XRE family transcriptional regulator, at 4,426,057 to 4,426,761
_RS20435
RR42_RS20440: RR42_RS20440 - histidine kinase, at 4,426,814 to 4,429,282
_RS20440
RR42_RS20445: RR42_RS20445 - hypothetical protein, at 4,429,279 to 4,429,998
_RS20445
RR42_RS20450: RR42_RS20450 - 16S rRNA methyltransferase, at 4,430,174 to 4,431,526
_RS20450
Position (kb) 4426
4427
4428
4429
4430 Strain fitness (log2 ratio) -2
-1
0
1 at 4426.762 kb on - strand at 4426.762 kb on - strand at 4426.762 kb on - strand at 4426.816 kb on - strand at 4426.816 kb on - strand at 4426.968 kb on + strand at 4426.987 kb on + strand at 4427.070 kb on + strand, within RR42_RS20440 at 4427.070 kb on + strand, within RR42_RS20440 at 4429.234 kb on - strand at 4430.200 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.01 mg/ml remove 4,426,762 - -0.5 4,426,762 - -0.3 4,426,762 - -1.6 4,426,816 - -0.4 4,426,816 - +0.2 4,426,968 + -1.9 4,426,987 + +0.0 4,427,070 + RR42_RS20440 0.10 -0.3 4,427,070 + RR42_RS20440 0.10 -1.3 4,429,234 - -1.6 4,430,200 - +0.7
Or see this region's nucleotide sequence