Experiment: R2A with A22 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS20305 and RR42_RS20310 are separated by 261 nucleotides RR42_RS20310 and RR42_RS20315 are separated by 69 nucleotides
RR42_RS20305: RR42_RS20305 - metal ABC transporter ATPase, at 4,392,439 to 4,394,910
_RS20305
RR42_RS20310: RR42_RS20310 - copper-binding protein, at 4,395,172 to 4,395,384
_RS20310
RR42_RS20315: RR42_RS20315 - membrane protein, at 4,395,454 to 4,396,182
_RS20315
Position (kb)
4395
4396 Strain fitness (log2 ratio)
-2
-1
0
1 at 4394.205 kb on + strand, within RR42_RS20305 at 4394.205 kb on + strand, within RR42_RS20305 at 4394.206 kb on - strand, within RR42_RS20305 at 4394.246 kb on + strand, within RR42_RS20305 at 4394.366 kb on - strand, within RR42_RS20305 at 4394.366 kb on - strand, within RR42_RS20305 at 4394.366 kb on - strand, within RR42_RS20305 at 4394.366 kb on - strand, within RR42_RS20305 at 4394.462 kb on + strand, within RR42_RS20305 at 4394.666 kb on + strand at 4394.955 kb on + strand at 4394.955 kb on + strand at 4394.956 kb on - strand at 4394.956 kb on - strand at 4394.956 kb on - strand at 4395.070 kb on + strand at 4395.153 kb on + strand at 4395.154 kb on - strand at 4395.296 kb on + strand, within RR42_RS20310 at 4395.455 kb on + strand at 4395.510 kb on + strand at 4395.588 kb on - strand, within RR42_RS20315 at 4395.629 kb on + strand, within RR42_RS20315 at 4395.629 kb on + strand, within RR42_RS20315 at 4395.629 kb on + strand, within RR42_RS20315 at 4395.630 kb on - strand, within RR42_RS20315 at 4395.630 kb on - strand, within RR42_RS20315 at 4395.755 kb on + strand, within RR42_RS20315 at 4395.756 kb on - strand, within RR42_RS20315 at 4395.756 kb on - strand, within RR42_RS20315 at 4395.756 kb on - strand, within RR42_RS20315 at 4395.776 kb on + strand, within RR42_RS20315 at 4395.776 kb on + strand, within RR42_RS20315 at 4395.776 kb on + strand, within RR42_RS20315 at 4395.776 kb on + strand, within RR42_RS20315 at 4395.777 kb on - strand, within RR42_RS20315 at 4395.777 kb on - strand, within RR42_RS20315 at 4395.777 kb on - strand, within RR42_RS20315 at 4395.777 kb on - strand, within RR42_RS20315 at 4395.812 kb on + strand, within RR42_RS20315 at 4395.813 kb on - strand, within RR42_RS20315 at 4395.863 kb on + strand, within RR42_RS20315 at 4395.863 kb on + strand, within RR42_RS20315 at 4395.864 kb on - strand, within RR42_RS20315 at 4395.933 kb on + strand, within RR42_RS20315 at 4395.942 kb on + strand, within RR42_RS20315 at 4396.038 kb on + strand, within RR42_RS20315 at 4396.039 kb on - strand, within RR42_RS20315 at 4396.101 kb on + strand, within RR42_RS20315 at 4396.101 kb on + strand, within RR42_RS20315 at 4396.102 kb on - strand, within RR42_RS20315 at 4396.102 kb on - strand, within RR42_RS20315 at 4396.102 kb on - strand, within RR42_RS20315 at 4396.141 kb on + strand at 4396.141 kb on + strand at 4396.141 kb on + strand at 4396.142 kb on - strand at 4396.142 kb on - strand at 4396.142 kb on - strand at 4396.151 kb on - strand at 4396.151 kb on - strand at 4396.189 kb on + strand at 4396.190 kb on - strand at 4396.190 kb on - strand at 4396.190 kb on - strand at 4396.198 kb on + strand at 4396.198 kb on + strand at 4396.198 kb on + strand at 4396.198 kb on + strand at 4396.199 kb on - strand at 4396.199 kb on - strand at 4396.199 kb on - strand at 4396.209 kb on + strand at 4396.226 kb on - strand at 4396.259 kb on + strand at 4396.261 kb on + strand at 4396.264 kb on + strand at 4396.265 kb on - strand at 4396.265 kb on - strand at 4396.273 kb on - strand at 4396.346 kb on + strand at 4396.381 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.01 mg/ml remove 4,394,205 + RR42_RS20305 0.71 -1.2 4,394,205 + RR42_RS20305 0.71 +0.4 4,394,206 - RR42_RS20305 0.71 -0.5 4,394,246 + RR42_RS20305 0.73 +0.8 4,394,366 - RR42_RS20305 0.78 +0.2 4,394,366 - RR42_RS20305 0.78 +1.4 4,394,366 - RR42_RS20305 0.78 -1.9 4,394,366 - RR42_RS20305 0.78 -1.5 4,394,462 + RR42_RS20305 0.82 +0.1 4,394,666 + -0.7 4,394,955 + -0.2 4,394,955 + -2.2 4,394,956 - +1.6 4,394,956 - -1.8 4,394,956 - +0.2 4,395,070 + +0.3 4,395,153 + -0.8 4,395,154 - -0.0 4,395,296 + RR42_RS20310 0.58 +1.2 4,395,455 + -0.1 4,395,510 + -0.7 4,395,588 - RR42_RS20315 0.18 -0.1 4,395,629 + RR42_RS20315 0.24 +0.1 4,395,629 + RR42_RS20315 0.24 +0.5 4,395,629 + RR42_RS20315 0.24 -0.2 4,395,630 - RR42_RS20315 0.24 +1.2 4,395,630 - RR42_RS20315 0.24 -1.1 4,395,755 + RR42_RS20315 0.41 -0.9 4,395,756 - RR42_RS20315 0.41 +1.2 4,395,756 - RR42_RS20315 0.41 +1.3 4,395,756 - RR42_RS20315 0.41 -1.9 4,395,776 + RR42_RS20315 0.44 -0.0 4,395,776 + RR42_RS20315 0.44 +0.2 4,395,776 + RR42_RS20315 0.44 +0.4 4,395,776 + RR42_RS20315 0.44 -0.6 4,395,777 - RR42_RS20315 0.44 -0.4 4,395,777 - RR42_RS20315 0.44 +0.1 4,395,777 - RR42_RS20315 0.44 -0.7 4,395,777 - RR42_RS20315 0.44 -0.8 4,395,812 + RR42_RS20315 0.49 +0.7 4,395,813 - RR42_RS20315 0.49 -0.3 4,395,863 + RR42_RS20315 0.56 -0.5 4,395,863 + RR42_RS20315 0.56 -0.9 4,395,864 - RR42_RS20315 0.56 -1.1 4,395,933 + RR42_RS20315 0.66 -0.9 4,395,942 + RR42_RS20315 0.67 -0.3 4,396,038 + RR42_RS20315 0.80 +0.9 4,396,039 - RR42_RS20315 0.80 +1.3 4,396,101 + RR42_RS20315 0.89 -1.6 4,396,101 + RR42_RS20315 0.89 +0.2 4,396,102 - RR42_RS20315 0.89 +1.5 4,396,102 - RR42_RS20315 0.89 -0.2 4,396,102 - RR42_RS20315 0.89 +0.7 4,396,141 + -0.4 4,396,141 + -0.1 4,396,141 + -1.8 4,396,142 - -0.1 4,396,142 - +0.2 4,396,142 - -0.7 4,396,151 - -1.1 4,396,151 - -0.2 4,396,189 + +0.8 4,396,190 - +0.0 4,396,190 - +0.1 4,396,190 - +0.4 4,396,198 + -1.7 4,396,198 + -0.7 4,396,198 + -0.3 4,396,198 + +0.6 4,396,199 - -0.1 4,396,199 - -0.9 4,396,199 - +0.6 4,396,209 + +1.5 4,396,226 - -0.0 4,396,259 + -0.9 4,396,261 + +0.1 4,396,264 + +1.0 4,396,265 - -1.2 4,396,265 - -1.5 4,396,273 - +0.5 4,396,346 + -0.1 4,396,381 - +0.4
Or see this region's nucleotide sequence