Experiment: R2A with A22 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS20200 and RR42_RS20205 are separated by 52 nucleotides RR42_RS20205 and RR42_RS20210 are separated by 68 nucleotides RR42_RS20210 and RR42_RS20215 are separated by 0 nucleotides
RR42_RS20200: RR42_RS20200 - chromosome partitioning protein ParB, at 4,368,708 to 4,369,610
_RS20200
RR42_RS20205: RR42_RS20205 - chromosome partitioning protein, at 4,369,663 to 4,370,436
_RS20205
RR42_RS20210: RR42_RS20210 - 16S rRNA methyltransferase, at 4,370,505 to 4,371,212
_RS20210
RR42_RS20215: RR42_RS20215 - tRNA uridine 5-carboxymethylaminomethyl modification protein, at 4,371,213 to 4,373,153
_RS20215
Position (kb) 4369
4370
4371 Strain fitness (log2 ratio) -2
-1
0
1
2 at 4368.690 kb on - strand at 4369.624 kb on - strand at 4369.764 kb on - strand, within RR42_RS20205 at 4369.947 kb on + strand, within RR42_RS20205 at 4369.948 kb on - strand, within RR42_RS20205 at 4369.983 kb on - strand, within RR42_RS20205 at 4370.426 kb on - strand at 4370.437 kb on - strand at 4370.437 kb on - strand at 4370.437 kb on - strand at 4371.062 kb on - strand, within RR42_RS20210 at 4371.062 kb on - strand, within RR42_RS20210 at 4371.062 kb on - strand, within RR42_RS20210 at 4371.062 kb on - strand, within RR42_RS20210 at 4371.095 kb on - strand, within RR42_RS20210 at 4371.246 kb on - strand at 4371.398 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.01 mg/ml remove 4,368,690 - -0.8 4,369,624 - -1.2 4,369,764 - RR42_RS20205 0.13 -2.4 4,369,947 + RR42_RS20205 0.37 -2.2 4,369,948 - RR42_RS20205 0.37 -1.2 4,369,983 - RR42_RS20205 0.41 +0.1 4,370,426 - -1.3 4,370,437 - -0.4 4,370,437 - -0.1 4,370,437 - -1.1 4,371,062 - RR42_RS20210 0.79 -0.6 4,371,062 - RR42_RS20210 0.79 +0.5 4,371,062 - RR42_RS20210 0.79 +1.9 4,371,062 - RR42_RS20210 0.79 +0.2 4,371,095 - RR42_RS20210 0.83 +0.4 4,371,246 - -0.5 4,371,398 - -1.4
Or see this region's nucleotide sequence