Experiment: R2A with A22 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS19850 and RR42_RS19855 are separated by 121 nucleotides RR42_RS19855 and RR42_RS19860 are separated by 51 nucleotides RR42_RS19860 and RR42_RS19865 are separated by 88 nucleotides
RR42_RS19850: RR42_RS19850 - glyoxylate carboligase, at 4,284,878 to 4,286,653
_RS19850
RR42_RS19855: RR42_RS19855 - hydroxypyruvate isomerase, at 4,286,775 to 4,287,557
_RS19855
RR42_RS19860: RR42_RS19860 - tartronate semialdehyde reductase, at 4,287,609 to 4,288,535
_RS19860
RR42_RS19865: RR42_RS19865 - hydroxypyruvate reductase, at 4,288,624 to 4,289,937
_RS19865
Position (kb) 4287
4288
4289 Strain fitness (log2 ratio) -2
-1
0
1
2 at 4286.651 kb on + strand at 4286.652 kb on - strand at 4286.652 kb on - strand at 4287.051 kb on + strand, within RR42_RS19855 at 4287.051 kb on + strand, within RR42_RS19855 at 4287.294 kb on + strand, within RR42_RS19855 at 4287.303 kb on + strand, within RR42_RS19855 at 4287.312 kb on + strand, within RR42_RS19855 at 4287.313 kb on - strand, within RR42_RS19855 at 4287.313 kb on - strand, within RR42_RS19855 at 4287.313 kb on - strand, within RR42_RS19855 at 4287.313 kb on - strand, within RR42_RS19855 at 4287.496 kb on - strand at 4287.555 kb on + strand at 4287.636 kb on - strand at 4287.981 kb on + strand, within RR42_RS19860 at 4288.313 kb on + strand, within RR42_RS19860 at 4288.533 kb on + strand at 4288.574 kb on - strand at 4288.793 kb on + strand, within RR42_RS19865 at 4288.793 kb on + strand, within RR42_RS19865 at 4289.108 kb on + strand, within RR42_RS19865 at 4289.109 kb on - strand, within RR42_RS19865 at 4289.109 kb on - strand, within RR42_RS19865 at 4289.320 kb on + strand, within RR42_RS19865 at 4289.320 kb on + strand, within RR42_RS19865 at 4289.321 kb on - strand, within RR42_RS19865
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.01 mg/ml remove 4,286,651 + +0.1 4,286,652 - -0.2 4,286,652 - -0.4 4,287,051 + RR42_RS19855 0.35 -1.1 4,287,051 + RR42_RS19855 0.35 -1.0 4,287,294 + RR42_RS19855 0.66 +1.9 4,287,303 + RR42_RS19855 0.67 +0.1 4,287,312 + RR42_RS19855 0.69 +0.1 4,287,313 - RR42_RS19855 0.69 +1.4 4,287,313 - RR42_RS19855 0.69 -0.5 4,287,313 - RR42_RS19855 0.69 +0.9 4,287,313 - RR42_RS19855 0.69 -0.7 4,287,496 - -0.4 4,287,555 + -0.6 4,287,636 - -0.0 4,287,981 + RR42_RS19860 0.40 -0.9 4,288,313 + RR42_RS19860 0.76 -1.6 4,288,533 + -0.5 4,288,574 - +0.6 4,288,793 + RR42_RS19865 0.13 +0.3 4,288,793 + RR42_RS19865 0.13 +0.2 4,289,108 + RR42_RS19865 0.37 +0.2 4,289,109 - RR42_RS19865 0.37 -2.4 4,289,109 - RR42_RS19865 0.37 +0.7 4,289,320 + RR42_RS19865 0.53 -0.6 4,289,320 + RR42_RS19865 0.53 +0.3 4,289,321 - RR42_RS19865 0.53 -0.7
Or see this region's nucleotide sequence