Experiment: R2A with A22 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS19520 and RR42_RS19525 are separated by 8 nucleotides RR42_RS19525 and RR42_RS19530 are separated by 16 nucleotides
RR42_RS19520: RR42_RS19520 - general secretion pathway protein GspD, at 4,213,687 to 4,216,116
_RS19520
RR42_RS19525: RR42_RS19525 - general secretion pathway protein GspE, at 4,216,125 to 4,217,672
_RS19525
RR42_RS19530: RR42_RS19530 - general secretion pathway protein GspF, at 4,217,689 to 4,218,906
_RS19530
Position (kb)
4216
4217
4218 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4215.386 kb on - strand, within RR42_RS19520 at 4215.647 kb on - strand, within RR42_RS19520 at 4215.647 kb on - strand, within RR42_RS19520 at 4215.763 kb on + strand, within RR42_RS19520 at 4215.764 kb on - strand, within RR42_RS19520 at 4215.764 kb on - strand, within RR42_RS19520 at 4215.812 kb on - strand, within RR42_RS19520 at 4215.818 kb on - strand, within RR42_RS19520 at 4215.819 kb on + strand, within RR42_RS19520 at 4215.819 kb on + strand, within RR42_RS19520 at 4215.820 kb on - strand, within RR42_RS19520 at 4215.820 kb on - strand, within RR42_RS19520 at 4215.820 kb on - strand, within RR42_RS19520 at 4215.820 kb on - strand, within RR42_RS19520 at 4215.820 kb on - strand, within RR42_RS19520 at 4215.855 kb on + strand, within RR42_RS19520 at 4215.877 kb on - strand at 4216.216 kb on - strand at 4216.217 kb on - strand at 4216.392 kb on + strand, within RR42_RS19525 at 4216.392 kb on + strand, within RR42_RS19525 at 4216.978 kb on - strand, within RR42_RS19525 at 4217.043 kb on + strand, within RR42_RS19525 at 4217.472 kb on + strand, within RR42_RS19525 at 4217.491 kb on + strand, within RR42_RS19525 at 4217.493 kb on + strand, within RR42_RS19525 at 4217.494 kb on - strand, within RR42_RS19525 at 4217.494 kb on - strand, within RR42_RS19525 at 4217.670 kb on + strand at 4217.670 kb on + strand at 4217.698 kb on + strand at 4217.698 kb on + strand at 4217.705 kb on - strand at 4217.783 kb on + strand at 4217.986 kb on + strand, within RR42_RS19530 at 4217.986 kb on + strand, within RR42_RS19530 at 4217.986 kb on + strand, within RR42_RS19530 at 4217.986 kb on + strand, within RR42_RS19530 at 4217.986 kb on + strand, within RR42_RS19530 at 4217.986 kb on + strand, within RR42_RS19530 at 4217.987 kb on - strand, within RR42_RS19530 at 4217.987 kb on - strand, within RR42_RS19530 at 4217.987 kb on - strand, within RR42_RS19530 at 4217.987 kb on - strand, within RR42_RS19530 at 4218.081 kb on + strand, within RR42_RS19530 at 4218.086 kb on - strand, within RR42_RS19530 at 4218.086 kb on - strand, within RR42_RS19530 at 4218.154 kb on + strand, within RR42_RS19530 at 4218.155 kb on - strand, within RR42_RS19530 at 4218.155 kb on - strand, within RR42_RS19530 at 4218.155 kb on - strand, within RR42_RS19530 at 4218.155 kb on - strand, within RR42_RS19530 at 4218.155 kb on - strand, within RR42_RS19530 at 4218.201 kb on + strand, within RR42_RS19530 at 4218.201 kb on + strand, within RR42_RS19530 at 4218.202 kb on - strand, within RR42_RS19530 at 4218.202 kb on - strand, within RR42_RS19530 at 4218.205 kb on + strand, within RR42_RS19530 at 4218.259 kb on + strand, within RR42_RS19530 at 4218.259 kb on + strand, within RR42_RS19530 at 4218.259 kb on + strand, within RR42_RS19530 at 4218.259 kb on + strand, within RR42_RS19530 at 4218.259 kb on + strand, within RR42_RS19530 at 4218.259 kb on + strand, within RR42_RS19530 at 4218.259 kb on + strand, within RR42_RS19530 at 4218.259 kb on + strand, within RR42_RS19530 at 4218.260 kb on - strand, within RR42_RS19530 at 4218.260 kb on - strand, within RR42_RS19530 at 4218.260 kb on - strand, within RR42_RS19530 at 4218.260 kb on - strand, within RR42_RS19530 at 4218.260 kb on - strand, within RR42_RS19530 at 4218.260 kb on - strand, within RR42_RS19530 at 4218.260 kb on - strand, within RR42_RS19530 at 4218.260 kb on - strand, within RR42_RS19530 at 4218.404 kb on + strand, within RR42_RS19530 at 4218.404 kb on + strand, within RR42_RS19530 at 4218.493 kb on + strand, within RR42_RS19530 at 4218.493 kb on + strand, within RR42_RS19530 at 4218.493 kb on + strand, within RR42_RS19530 at 4218.494 kb on - strand, within RR42_RS19530 at 4218.495 kb on + strand, within RR42_RS19530 at 4218.495 kb on + strand, within RR42_RS19530 at 4218.495 kb on + strand, within RR42_RS19530 at 4218.496 kb on - strand, within RR42_RS19530
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.01 mg/ml remove 4,215,386 - RR42_RS19520 0.70 -0.0 4,215,647 - RR42_RS19520 0.81 -0.8 4,215,647 - RR42_RS19520 0.81 +0.0 4,215,763 + RR42_RS19520 0.85 -0.9 4,215,764 - RR42_RS19520 0.85 -1.1 4,215,764 - RR42_RS19520 0.85 +0.4 4,215,812 - RR42_RS19520 0.87 -1.9 4,215,818 - RR42_RS19520 0.88 -0.1 4,215,819 + RR42_RS19520 0.88 -0.3 4,215,819 + RR42_RS19520 0.88 -1.4 4,215,820 - RR42_RS19520 0.88 -1.8 4,215,820 - RR42_RS19520 0.88 -1.1 4,215,820 - RR42_RS19520 0.88 -1.2 4,215,820 - RR42_RS19520 0.88 -0.8 4,215,820 - RR42_RS19520 0.88 -1.6 4,215,855 + RR42_RS19520 0.89 -0.6 4,215,877 - -1.7 4,216,216 - -0.2 4,216,217 - -1.4 4,216,392 + RR42_RS19525 0.17 +0.4 4,216,392 + RR42_RS19525 0.17 -0.6 4,216,978 - RR42_RS19525 0.55 -0.4 4,217,043 + RR42_RS19525 0.59 -0.6 4,217,472 + RR42_RS19525 0.87 -0.2 4,217,491 + RR42_RS19525 0.88 -1.2 4,217,493 + RR42_RS19525 0.88 -2.0 4,217,494 - RR42_RS19525 0.88 -0.4 4,217,494 - RR42_RS19525 0.88 -1.2 4,217,670 + +0.5 4,217,670 + -0.1 4,217,698 + -0.9 4,217,698 + -0.6 4,217,705 - +0.6 4,217,783 + -1.6 4,217,986 + RR42_RS19530 0.24 -0.8 4,217,986 + RR42_RS19530 0.24 +0.1 4,217,986 + RR42_RS19530 0.24 -0.9 4,217,986 + RR42_RS19530 0.24 -0.4 4,217,986 + RR42_RS19530 0.24 +2.1 4,217,986 + RR42_RS19530 0.24 -0.0 4,217,987 - RR42_RS19530 0.24 -0.9 4,217,987 - RR42_RS19530 0.24 -0.7 4,217,987 - RR42_RS19530 0.24 -2.2 4,217,987 - RR42_RS19530 0.24 -0.5 4,218,081 + RR42_RS19530 0.32 -1.1 4,218,086 - RR42_RS19530 0.33 -0.4 4,218,086 - RR42_RS19530 0.33 -1.4 4,218,154 + RR42_RS19530 0.38 -1.4 4,218,155 - RR42_RS19530 0.38 -1.6 4,218,155 - RR42_RS19530 0.38 -1.0 4,218,155 - RR42_RS19530 0.38 -2.5 4,218,155 - RR42_RS19530 0.38 -0.3 4,218,155 - RR42_RS19530 0.38 +2.4 4,218,201 + RR42_RS19530 0.42 -1.5 4,218,201 + RR42_RS19530 0.42 -0.2 4,218,202 - RR42_RS19530 0.42 -1.9 4,218,202 - RR42_RS19530 0.42 +0.1 4,218,205 + RR42_RS19530 0.42 -1.0 4,218,259 + RR42_RS19530 0.47 -0.1 4,218,259 + RR42_RS19530 0.47 -1.8 4,218,259 + RR42_RS19530 0.47 +0.4 4,218,259 + RR42_RS19530 0.47 -0.8 4,218,259 + RR42_RS19530 0.47 -1.2 4,218,259 + RR42_RS19530 0.47 -2.5 4,218,259 + RR42_RS19530 0.47 -2.2 4,218,259 + RR42_RS19530 0.47 -0.6 4,218,260 - RR42_RS19530 0.47 -1.2 4,218,260 - RR42_RS19530 0.47 -0.1 4,218,260 - RR42_RS19530 0.47 -1.8 4,218,260 - RR42_RS19530 0.47 +0.1 4,218,260 - RR42_RS19530 0.47 -0.4 4,218,260 - RR42_RS19530 0.47 -0.4 4,218,260 - RR42_RS19530 0.47 -2.6 4,218,260 - RR42_RS19530 0.47 -1.3 4,218,404 + RR42_RS19530 0.59 -0.8 4,218,404 + RR42_RS19530 0.59 -0.9 4,218,493 + RR42_RS19530 0.66 -0.9 4,218,493 + RR42_RS19530 0.66 -0.5 4,218,493 + RR42_RS19530 0.66 -1.5 4,218,494 - RR42_RS19530 0.66 -1.3 4,218,495 + RR42_RS19530 0.66 -1.8 4,218,495 + RR42_RS19530 0.66 -0.2 4,218,495 + RR42_RS19530 0.66 -1.2 4,218,496 - RR42_RS19530 0.66 -0.6
Or see this region's nucleotide sequence