Experiment: R2A with A22 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS19505 and RR42_RS19510 are separated by 14 nucleotides RR42_RS19510 and RR42_RS19515 overlap by 1 nucleotides RR42_RS19515 and RR42_RS19520 are separated by 53 nucleotides
RR42_RS19505: RR42_RS19505 - general secretion pathway protein GspL, at 4,210,744 to 4,212,153
_RS19505
RR42_RS19510: RR42_RS19510 - general secretion pathway protein GspM, at 4,212,168 to 4,212,794
_RS19510
RR42_RS19515: RR42_RS19515 - general secretion pathway protein GspN, at 4,212,794 to 4,213,633
_RS19515
RR42_RS19520: RR42_RS19520 - general secretion pathway protein GspD, at 4,213,687 to 4,216,116
_RS19520
Position (kb) 4212
4213
4214 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 4212.084 kb on + strand at 4212.384 kb on + strand, within RR42_RS19510 at 4212.717 kb on + strand, within RR42_RS19510 at 4212.718 kb on - strand, within RR42_RS19510 at 4212.718 kb on - strand, within RR42_RS19510 at 4212.718 kb on - strand, within RR42_RS19510 at 4213.246 kb on + strand, within RR42_RS19515 at 4213.369 kb on - strand, within RR42_RS19515 at 4213.383 kb on + strand, within RR42_RS19515 at 4213.384 kb on - strand, within RR42_RS19515 at 4213.384 kb on - strand, within RR42_RS19515 at 4213.384 kb on - strand, within RR42_RS19515 at 4213.384 kb on - strand, within RR42_RS19515 at 4213.384 kb on - strand, within RR42_RS19515 at 4213.471 kb on + strand, within RR42_RS19515 at 4213.471 kb on + strand, within RR42_RS19515 at 4213.472 kb on - strand, within RR42_RS19515 at 4213.472 kb on - strand, within RR42_RS19515 at 4213.585 kb on - strand at 4213.585 kb on - strand at 4213.687 kb on - strand at 4213.748 kb on + strand at 4213.748 kb on + strand at 4214.038 kb on + strand, within RR42_RS19520 at 4214.039 kb on - strand, within RR42_RS19520 at 4214.039 kb on - strand, within RR42_RS19520 at 4214.039 kb on - strand, within RR42_RS19520 at 4214.072 kb on + strand, within RR42_RS19520 at 4214.072 kb on + strand, within RR42_RS19520 at 4214.072 kb on + strand, within RR42_RS19520 at 4214.073 kb on - strand, within RR42_RS19520 at 4214.073 kb on - strand, within RR42_RS19520 at 4214.173 kb on + strand, within RR42_RS19520 at 4214.173 kb on + strand, within RR42_RS19520 at 4214.173 kb on + strand, within RR42_RS19520 at 4214.173 kb on + strand, within RR42_RS19520 at 4214.173 kb on + strand, within RR42_RS19520 at 4214.173 kb on + strand, within RR42_RS19520 at 4214.173 kb on + strand, within RR42_RS19520 at 4214.174 kb on - strand, within RR42_RS19520 at 4214.174 kb on - strand, within RR42_RS19520 at 4214.174 kb on - strand, within RR42_RS19520 at 4214.174 kb on - strand, within RR42_RS19520 at 4214.174 kb on - strand, within RR42_RS19520 at 4214.275 kb on + strand, within RR42_RS19520 at 4214.275 kb on + strand, within RR42_RS19520 at 4214.276 kb on - strand, within RR42_RS19520 at 4214.276 kb on - strand, within RR42_RS19520 at 4214.276 kb on - strand, within RR42_RS19520 at 4214.276 kb on - strand, within RR42_RS19520 at 4214.572 kb on + strand, within RR42_RS19520 at 4214.573 kb on - strand, within RR42_RS19520 at 4214.589 kb on + strand, within RR42_RS19520 at 4214.589 kb on + strand, within RR42_RS19520 at 4214.590 kb on - strand, within RR42_RS19520 at 4214.590 kb on - strand, within RR42_RS19520 at 4214.590 kb on - strand, within RR42_RS19520
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.01 mg/ml remove 4,212,084 + +0.6 4,212,384 + RR42_RS19510 0.34 -2.1 4,212,717 + RR42_RS19510 0.88 -0.4 4,212,718 - RR42_RS19510 0.88 -1.6 4,212,718 - RR42_RS19510 0.88 -1.9 4,212,718 - RR42_RS19510 0.88 -1.3 4,213,246 + RR42_RS19515 0.54 -1.1 4,213,369 - RR42_RS19515 0.68 -1.3 4,213,383 + RR42_RS19515 0.70 -1.4 4,213,384 - RR42_RS19515 0.70 +0.1 4,213,384 - RR42_RS19515 0.70 -1.3 4,213,384 - RR42_RS19515 0.70 +0.2 4,213,384 - RR42_RS19515 0.70 -1.7 4,213,384 - RR42_RS19515 0.70 -1.1 4,213,471 + RR42_RS19515 0.81 -1.4 4,213,471 + RR42_RS19515 0.81 -1.9 4,213,472 - RR42_RS19515 0.81 -0.9 4,213,472 - RR42_RS19515 0.81 -0.6 4,213,585 - +0.2 4,213,585 - -1.5 4,213,687 - -1.9 4,213,748 + -0.1 4,213,748 + -1.2 4,214,038 + RR42_RS19520 0.14 -1.6 4,214,039 - RR42_RS19520 0.14 -0.7 4,214,039 - RR42_RS19520 0.14 -1.2 4,214,039 - RR42_RS19520 0.14 -0.6 4,214,072 + RR42_RS19520 0.16 +0.3 4,214,072 + RR42_RS19520 0.16 -0.3 4,214,072 + RR42_RS19520 0.16 -0.7 4,214,073 - RR42_RS19520 0.16 -1.8 4,214,073 - RR42_RS19520 0.16 +3.0 4,214,173 + RR42_RS19520 0.20 -0.3 4,214,173 + RR42_RS19520 0.20 -1.0 4,214,173 + RR42_RS19520 0.20 -2.1 4,214,173 + RR42_RS19520 0.20 -1.9 4,214,173 + RR42_RS19520 0.20 -1.0 4,214,173 + RR42_RS19520 0.20 -1.1 4,214,173 + RR42_RS19520 0.20 -2.1 4,214,174 - RR42_RS19520 0.20 -1.7 4,214,174 - RR42_RS19520 0.20 -0.5 4,214,174 - RR42_RS19520 0.20 -1.7 4,214,174 - RR42_RS19520 0.20 -2.2 4,214,174 - RR42_RS19520 0.20 -1.5 4,214,275 + RR42_RS19520 0.24 -2.5 4,214,275 + RR42_RS19520 0.24 -0.5 4,214,276 - RR42_RS19520 0.24 -2.6 4,214,276 - RR42_RS19520 0.24 -0.4 4,214,276 - RR42_RS19520 0.24 -2.2 4,214,276 - RR42_RS19520 0.24 -0.3 4,214,572 + RR42_RS19520 0.36 -0.4 4,214,573 - RR42_RS19520 0.36 +0.1 4,214,589 + RR42_RS19520 0.37 -1.0 4,214,589 + RR42_RS19520 0.37 -0.2 4,214,590 - RR42_RS19520 0.37 -1.1 4,214,590 - RR42_RS19520 0.37 -0.9 4,214,590 - RR42_RS19520 0.37 -1.6
Or see this region's nucleotide sequence