Experiment: R2A with A22 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS19490 and RR42_RS19495 are separated by 58 nucleotides RR42_RS19495 and RR42_RS19500 are separated by 20 nucleotides RR42_RS19500 and RR42_RS19505 are separated by 392 nucleotides
RR42_RS19490: RR42_RS19490 - general secretion pathway protein GspI, at 4,208,225 to 4,208,605
_RS19490
RR42_RS19495: RR42_RS19495 - general secretion pathway protein GspJ, at 4,208,664 to 4,209,335
_RS19495
RR42_RS19500: RR42_RS19500 - general secretion pathway protein GspK, at 4,209,356 to 4,210,351
_RS19500
RR42_RS19505: RR42_RS19505 - general secretion pathway protein GspL, at 4,210,744 to 4,212,153
_RS19505
Position (kb)
4209
4210
4211 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 4208.463 kb on - strand, within RR42_RS19490 at 4208.463 kb on - strand, within RR42_RS19490 at 4208.463 kb on - strand, within RR42_RS19490 at 4208.612 kb on + strand at 4208.622 kb on - strand at 4208.630 kb on + strand at 4208.630 kb on + strand at 4208.630 kb on + strand at 4208.630 kb on + strand at 4208.630 kb on + strand at 4208.631 kb on - strand at 4208.631 kb on - strand at 4208.812 kb on + strand, within RR42_RS19495 at 4208.812 kb on + strand, within RR42_RS19495 at 4208.813 kb on - strand, within RR42_RS19495 at 4208.813 kb on - strand, within RR42_RS19495 at 4208.813 kb on - strand, within RR42_RS19495 at 4208.813 kb on - strand, within RR42_RS19495 at 4208.814 kb on + strand, within RR42_RS19495 at 4208.814 kb on + strand, within RR42_RS19495 at 4208.815 kb on - strand, within RR42_RS19495 at 4208.815 kb on - strand, within RR42_RS19495 at 4208.815 kb on - strand, within RR42_RS19495 at 4208.958 kb on + strand, within RR42_RS19495 at 4209.331 kb on + strand at 4209.331 kb on + strand at 4209.459 kb on + strand, within RR42_RS19500 at 4209.459 kb on + strand, within RR42_RS19500 at 4209.862 kb on - strand, within RR42_RS19500 at 4210.195 kb on - strand, within RR42_RS19500 at 4210.195 kb on - strand, within RR42_RS19500 at 4210.205 kb on + strand, within RR42_RS19500 at 4210.206 kb on - strand, within RR42_RS19500 at 4210.206 kb on - strand, within RR42_RS19500 at 4210.206 kb on - strand, within RR42_RS19500 at 4210.217 kb on + strand, within RR42_RS19500 at 4210.218 kb on - strand, within RR42_RS19500 at 4210.218 kb on - strand, within RR42_RS19500 at 4210.269 kb on - strand at 4210.440 kb on + strand at 4210.440 kb on + strand at 4210.440 kb on + strand at 4210.440 kb on + strand at 4210.440 kb on + strand at 4210.440 kb on + strand at 4210.440 kb on + strand at 4210.441 kb on - strand at 4210.441 kb on - strand at 4210.441 kb on - strand at 4210.441 kb on - strand at 4210.441 kb on - strand at 4210.536 kb on + strand at 4210.536 kb on + strand at 4210.537 kb on - strand at 4210.537 kb on - strand at 4210.537 kb on - strand at 4210.537 kb on - strand at 4210.537 kb on - strand at 4210.540 kb on + strand at 4210.540 kb on + strand at 4210.540 kb on + strand at 4210.540 kb on + strand at 4210.540 kb on + strand at 4210.541 kb on - strand at 4210.541 kb on - strand at 4210.541 kb on - strand at 4210.541 kb on - strand at 4210.541 kb on - strand at 4210.541 kb on - strand at 4210.541 kb on - strand at 4210.541 kb on - strand at 4210.541 kb on - strand at 4210.541 kb on - strand at 4210.541 kb on - strand at 4210.570 kb on + strand at 4210.570 kb on + strand at 4210.570 kb on + strand at 4210.570 kb on + strand at 4210.571 kb on - strand at 4210.571 kb on - strand at 4210.571 kb on - strand at 4210.639 kb on + strand at 4210.731 kb on + strand at 4210.732 kb on - strand at 4210.740 kb on + strand at 4210.740 kb on + strand at 4210.740 kb on + strand at 4210.740 kb on + strand at 4210.741 kb on - strand at 4210.759 kb on + strand at 4210.759 kb on + strand at 4210.760 kb on - strand at 4210.760 kb on - strand at 4210.854 kb on + strand at 4211.073 kb on + strand, within RR42_RS19505
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.01 mg/ml remove 4,208,463 - RR42_RS19490 0.62 +0.2 4,208,463 - RR42_RS19490 0.62 -0.6 4,208,463 - RR42_RS19490 0.62 -1.7 4,208,612 + -0.5 4,208,622 - -2.5 4,208,630 + -1.4 4,208,630 + -0.5 4,208,630 + +0.2 4,208,630 + +0.4 4,208,630 + +0.4 4,208,631 - -2.8 4,208,631 - -3.4 4,208,812 + RR42_RS19495 0.22 -0.6 4,208,812 + RR42_RS19495 0.22 -0.6 4,208,813 - RR42_RS19495 0.22 -1.4 4,208,813 - RR42_RS19495 0.22 -0.3 4,208,813 - RR42_RS19495 0.22 -1.1 4,208,813 - RR42_RS19495 0.22 -1.3 4,208,814 + RR42_RS19495 0.22 +1.4 4,208,814 + RR42_RS19495 0.22 +0.5 4,208,815 - RR42_RS19495 0.22 -1.2 4,208,815 - RR42_RS19495 0.22 -0.2 4,208,815 - RR42_RS19495 0.22 -0.4 4,208,958 + RR42_RS19495 0.44 -1.7 4,209,331 + -1.8 4,209,331 + -0.9 4,209,459 + RR42_RS19500 0.10 -1.1 4,209,459 + RR42_RS19500 0.10 -1.2 4,209,862 - RR42_RS19500 0.51 -1.5 4,210,195 - RR42_RS19500 0.84 -0.7 4,210,195 - RR42_RS19500 0.84 -0.4 4,210,205 + RR42_RS19500 0.85 -2.8 4,210,206 - RR42_RS19500 0.85 -0.7 4,210,206 - RR42_RS19500 0.85 -1.4 4,210,206 - RR42_RS19500 0.85 -1.0 4,210,217 + RR42_RS19500 0.86 -0.7 4,210,218 - RR42_RS19500 0.87 -1.2 4,210,218 - RR42_RS19500 0.87 -3.4 4,210,269 - -0.6 4,210,440 + -0.6 4,210,440 + +0.5 4,210,440 + +0.5 4,210,440 + +0.0 4,210,440 + -0.1 4,210,440 + +0.7 4,210,440 + -0.7 4,210,441 - +0.1 4,210,441 - -0.3 4,210,441 - +0.6 4,210,441 - -0.9 4,210,441 - +0.2 4,210,536 + -0.2 4,210,536 + +1.4 4,210,537 - -1.4 4,210,537 - -0.1 4,210,537 - -1.4 4,210,537 - -1.8 4,210,537 - -1.2 4,210,540 + -0.6 4,210,540 + -0.9 4,210,540 + +0.1 4,210,540 + +0.5 4,210,540 + -0.3 4,210,541 - -1.5 4,210,541 - +0.1 4,210,541 - -0.3 4,210,541 - +1.4 4,210,541 - -2.3 4,210,541 - -0.4 4,210,541 - +0.4 4,210,541 - -2.4 4,210,541 - -1.4 4,210,541 - -1.8 4,210,541 - -0.6 4,210,570 + -0.9 4,210,570 + -0.2 4,210,570 + +0.7 4,210,570 + +0.0 4,210,571 - -1.5 4,210,571 - -0.1 4,210,571 - -2.2 4,210,639 + +0.0 4,210,731 + -0.4 4,210,732 - -2.0 4,210,740 + -0.8 4,210,740 + +0.7 4,210,740 + -1.4 4,210,740 + -1.4 4,210,741 - -2.0 4,210,759 + -3.3 4,210,759 + -0.4 4,210,760 - -1.0 4,210,760 - -1.5 4,210,854 + -2.4 4,211,073 + RR42_RS19505 0.23 -2.1
Or see this region's nucleotide sequence