Experiment: R2A with A22 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS18940 and RR42_RS18945 are separated by 247 nucleotides RR42_RS18945 and RR42_RS18950 are separated by 124 nucleotides RR42_RS18950 and RR42_RS18955 are separated by 203 nucleotides
RR42_RS18940: RR42_RS18940 - ABC transporter ATP-binding protein, at 4,100,577 to 4,101,530
_RS18940
RR42_RS18945: RR42_RS18945 - toluene tolerance protein, at 4,101,778 to 4,102,086
_RS18945
RR42_RS18950: RR42_RS18950 - hypothetical protein, at 4,102,211 to 4,102,834
_RS18950
RR42_RS18955: RR42_RS18955 - ABC transporter, at 4,103,038 to 4,103,868
_RS18955
Position (kb)
4101
4102
4103 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4100.960 kb on - strand, within RR42_RS18940 at 4100.960 kb on - strand, within RR42_RS18940 at 4101.224 kb on - strand, within RR42_RS18940 at 4101.224 kb on - strand, within RR42_RS18940 at 4101.303 kb on - strand, within RR42_RS18940 at 4101.303 kb on - strand, within RR42_RS18940 at 4101.303 kb on - strand, within RR42_RS18940 at 4101.640 kb on - strand at 4101.640 kb on - strand at 4101.640 kb on - strand at 4101.640 kb on - strand at 4101.640 kb on - strand at 4101.640 kb on - strand at 4101.640 kb on - strand at 4101.640 kb on - strand at 4101.642 kb on - strand at 4101.671 kb on - strand at 4101.671 kb on - strand at 4101.671 kb on - strand at 4101.671 kb on - strand at 4101.857 kb on + strand, within RR42_RS18945 at 4101.857 kb on + strand, within RR42_RS18945 at 4101.857 kb on + strand, within RR42_RS18945 at 4101.857 kb on + strand, within RR42_RS18945 at 4101.857 kb on + strand, within RR42_RS18945 at 4101.858 kb on - strand, within RR42_RS18945 at 4102.152 kb on + strand at 4102.152 kb on + strand at 4102.152 kb on + strand at 4102.153 kb on - strand at 4102.153 kb on - strand at 4102.311 kb on + strand, within RR42_RS18950 at 4102.311 kb on + strand, within RR42_RS18950 at 4102.311 kb on + strand, within RR42_RS18950 at 4102.312 kb on - strand, within RR42_RS18950 at 4102.312 kb on - strand, within RR42_RS18950 at 4102.312 kb on - strand, within RR42_RS18950 at 4102.312 kb on - strand, within RR42_RS18950 at 4102.350 kb on + strand, within RR42_RS18950 at 4102.351 kb on - strand, within RR42_RS18950 at 4102.351 kb on - strand, within RR42_RS18950 at 4102.351 kb on - strand, within RR42_RS18950 at 4102.351 kb on - strand, within RR42_RS18950 at 4102.464 kb on + strand, within RR42_RS18950 at 4102.515 kb on + strand, within RR42_RS18950 at 4102.515 kb on + strand, within RR42_RS18950 at 4102.515 kb on + strand, within RR42_RS18950 at 4102.515 kb on + strand, within RR42_RS18950 at 4102.515 kb on + strand, within RR42_RS18950 at 4102.515 kb on + strand, within RR42_RS18950 at 4102.516 kb on - strand, within RR42_RS18950 at 4102.516 kb on - strand, within RR42_RS18950 at 4102.805 kb on - strand at 4102.834 kb on + strand at 4102.835 kb on - strand at 4102.835 kb on - strand at 4102.835 kb on - strand at 4102.835 kb on - strand at 4102.845 kb on + strand at 4102.846 kb on - strand at 4102.875 kb on + strand at 4102.876 kb on - strand at 4102.876 kb on - strand at 4102.876 kb on - strand at 4102.886 kb on + strand at 4102.886 kb on + strand at 4102.886 kb on + strand at 4102.886 kb on + strand at 4102.886 kb on + strand at 4102.886 kb on + strand at 4102.886 kb on + strand at 4102.886 kb on + strand at 4102.886 kb on + strand at 4102.886 kb on + strand at 4102.886 kb on + strand at 4102.886 kb on + strand at 4102.886 kb on + strand at 4102.887 kb on - strand at 4102.887 kb on - strand at 4102.887 kb on - strand at 4102.887 kb on - strand at 4102.887 kb on - strand at 4102.887 kb on - strand at 4103.061 kb on + strand at 4103.062 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.01 mg/ml remove 4,100,960 - RR42_RS18940 0.40 -0.1 4,100,960 - RR42_RS18940 0.40 -0.2 4,101,224 - RR42_RS18940 0.68 +0.5 4,101,224 - RR42_RS18940 0.68 -0.3 4,101,303 - RR42_RS18940 0.76 -1.5 4,101,303 - RR42_RS18940 0.76 +0.8 4,101,303 - RR42_RS18940 0.76 +0.4 4,101,640 - +2.3 4,101,640 - +0.2 4,101,640 - -0.3 4,101,640 - +0.4 4,101,640 - -0.3 4,101,640 - -0.4 4,101,640 - +0.9 4,101,640 - -1.7 4,101,642 - +1.1 4,101,671 - -1.0 4,101,671 - +0.7 4,101,671 - +0.3 4,101,671 - -0.5 4,101,857 + RR42_RS18945 0.26 -1.3 4,101,857 + RR42_RS18945 0.26 -0.3 4,101,857 + RR42_RS18945 0.26 -0.6 4,101,857 + RR42_RS18945 0.26 -2.1 4,101,857 + RR42_RS18945 0.26 -1.2 4,101,858 - RR42_RS18945 0.26 -0.4 4,102,152 + +0.9 4,102,152 + -1.3 4,102,152 + -0.3 4,102,153 - -0.8 4,102,153 - +0.7 4,102,311 + RR42_RS18950 0.16 -0.8 4,102,311 + RR42_RS18950 0.16 -0.7 4,102,311 + RR42_RS18950 0.16 -0.5 4,102,312 - RR42_RS18950 0.16 -0.6 4,102,312 - RR42_RS18950 0.16 -0.2 4,102,312 - RR42_RS18950 0.16 +0.1 4,102,312 - RR42_RS18950 0.16 -0.2 4,102,350 + RR42_RS18950 0.22 -1.3 4,102,351 - RR42_RS18950 0.22 -1.3 4,102,351 - RR42_RS18950 0.22 +0.2 4,102,351 - RR42_RS18950 0.22 -0.8 4,102,351 - RR42_RS18950 0.22 -0.7 4,102,464 + RR42_RS18950 0.41 +0.7 4,102,515 + RR42_RS18950 0.49 -0.3 4,102,515 + RR42_RS18950 0.49 +0.6 4,102,515 + RR42_RS18950 0.49 -0.9 4,102,515 + RR42_RS18950 0.49 -0.3 4,102,515 + RR42_RS18950 0.49 -0.6 4,102,515 + RR42_RS18950 0.49 -0.9 4,102,516 - RR42_RS18950 0.49 -1.5 4,102,516 - RR42_RS18950 0.49 -1.3 4,102,805 - -1.3 4,102,834 + -0.7 4,102,835 - +0.5 4,102,835 - -1.8 4,102,835 - -0.3 4,102,835 - -0.6 4,102,845 + -1.0 4,102,846 - -0.9 4,102,875 + -0.1 4,102,876 - +0.1 4,102,876 - +0.0 4,102,876 - -0.0 4,102,886 + -0.1 4,102,886 + +0.1 4,102,886 + -0.2 4,102,886 + +0.5 4,102,886 + -0.1 4,102,886 + -1.5 4,102,886 + -0.2 4,102,886 + +0.2 4,102,886 + -0.2 4,102,886 + -0.1 4,102,886 + +0.2 4,102,886 + -0.2 4,102,886 + -0.4 4,102,887 - -0.5 4,102,887 - +0.1 4,102,887 - +1.5 4,102,887 - -1.3 4,102,887 - -0.4 4,102,887 - -0.3 4,103,061 + -1.0 4,103,062 - -0.2
Or see this region's nucleotide sequence