Experiment: R2A with A22 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS18420 and RR42_RS18425 are separated by 491 nucleotides RR42_RS18425 and RR42_RS18430 are separated by 677 nucleotides RR42_RS18430 and RR42_RS18435 overlap by 1 nucleotides
RR42_RS18420: RR42_RS18420 - hypothetical protein, at 3,990,602 to 3,991,171
_RS18420
RR42_RS18425: RR42_RS18425 - hypothetical protein, at 3,991,663 to 3,991,953
_RS18425
RR42_RS18430: RR42_RS18430 - prevent-host-death protein, at 3,992,631 to 3,992,900
_RS18430
RR42_RS18435: RR42_RS18435 - DNA-binding protein, at 3,992,900 to 3,993,313
_RS18435
Position (kb)
3991
3992 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 3990.787 kb on + strand, within RR42_RS18420 at 3990.860 kb on - strand, within RR42_RS18420 at 3990.894 kb on + strand, within RR42_RS18420 at 3990.928 kb on + strand, within RR42_RS18420 at 3990.928 kb on + strand, within RR42_RS18420 at 3990.929 kb on - strand, within RR42_RS18420 at 3990.946 kb on + strand, within RR42_RS18420 at 3990.967 kb on + strand, within RR42_RS18420 at 3990.968 kb on - strand, within RR42_RS18420 at 3990.991 kb on + strand, within RR42_RS18420 at 3990.991 kb on + strand, within RR42_RS18420 at 3990.991 kb on + strand, within RR42_RS18420 at 3991.011 kb on + strand, within RR42_RS18420 at 3991.011 kb on + strand, within RR42_RS18420 at 3991.012 kb on - strand, within RR42_RS18420 at 3991.029 kb on + strand, within RR42_RS18420 at 3991.029 kb on + strand, within RR42_RS18420 at 3991.031 kb on + strand, within RR42_RS18420 at 3991.031 kb on + strand, within RR42_RS18420 at 3991.031 kb on + strand, within RR42_RS18420 at 3991.032 kb on - strand, within RR42_RS18420 at 3991.032 kb on - strand, within RR42_RS18420 at 3991.032 kb on - strand, within RR42_RS18420 at 3991.032 kb on - strand, within RR42_RS18420 at 3991.240 kb on + strand at 3991.240 kb on + strand at 3991.240 kb on + strand at 3991.241 kb on - strand at 3991.241 kb on - strand at 3991.241 kb on - strand at 3991.241 kb on - strand at 3991.241 kb on - strand at 3991.252 kb on + strand at 3991.252 kb on + strand at 3991.329 kb on - strand at 3991.329 kb on - strand at 3991.358 kb on + strand at 3991.408 kb on + strand at 3991.426 kb on + strand at 3991.426 kb on + strand at 3991.426 kb on + strand at 3991.427 kb on - strand at 3991.427 kb on - strand at 3991.427 kb on - strand at 3991.429 kb on + strand at 3991.449 kb on - strand at 3991.483 kb on + strand at 3991.483 kb on + strand at 3991.515 kb on + strand at 3991.516 kb on - strand at 3991.516 kb on - strand at 3991.525 kb on + strand at 3991.525 kb on + strand at 3991.533 kb on - strand at 3991.864 kb on - strand, within RR42_RS18425 at 3991.902 kb on + strand, within RR42_RS18425 at 3991.935 kb on - strand at 3991.947 kb on + strand at 3991.947 kb on + strand at 3991.947 kb on + strand at 3991.947 kb on + strand at 3991.948 kb on - strand at 3991.948 kb on - strand at 3991.948 kb on - strand at 3991.948 kb on - strand at 3991.995 kb on - strand at 3992.027 kb on - strand at 3992.117 kb on + strand at 3992.117 kb on + strand at 3992.162 kb on + strand at 3992.162 kb on + strand at 3992.162 kb on + strand at 3992.163 kb on - strand at 3992.163 kb on - strand at 3992.163 kb on - strand at 3992.163 kb on - strand at 3992.165 kb on + strand at 3992.165 kb on + strand at 3992.165 kb on + strand at 3992.166 kb on - strand at 3992.166 kb on - strand at 3992.166 kb on - strand at 3992.167 kb on + strand at 3992.168 kb on - strand at 3992.233 kb on + strand at 3992.233 kb on + strand at 3992.233 kb on + strand at 3992.234 kb on - strand at 3992.264 kb on + strand at 3992.264 kb on + strand at 3992.264 kb on + strand at 3992.265 kb on - strand at 3992.265 kb on - strand at 3992.265 kb on - strand at 3992.296 kb on + strand at 3992.296 kb on + strand at 3992.297 kb on - strand at 3992.499 kb on + strand at 3992.499 kb on + strand at 3992.499 kb on + strand at 3992.500 kb on - strand at 3992.512 kb on + strand at 3992.512 kb on + strand at 3992.512 kb on + strand at 3992.513 kb on - strand at 3992.513 kb on - strand at 3992.513 kb on - strand at 3992.513 kb on - strand at 3992.513 kb on - strand at 3992.513 kb on - strand at 3992.558 kb on - strand at 3992.578 kb on - strand at 3992.595 kb on + strand at 3992.596 kb on - strand at 3992.604 kb on + strand at 3992.605 kb on - strand at 3992.614 kb on + strand at 3992.626 kb on + strand at 3992.626 kb on + strand at 3992.626 kb on + strand at 3992.765 kb on - strand, within RR42_RS18430 at 3992.765 kb on - strand, within RR42_RS18430 at 3992.899 kb on - strand at 3992.919 kb on + strand at 3992.920 kb on - strand at 3992.920 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.01 mg/ml remove 3,990,787 + RR42_RS18420 0.32 -2.5 3,990,860 - RR42_RS18420 0.45 -3.8 3,990,894 + RR42_RS18420 0.51 -4.5 3,990,928 + RR42_RS18420 0.57 -2.6 3,990,928 + RR42_RS18420 0.57 -2.5 3,990,929 - RR42_RS18420 0.57 -2.6 3,990,946 + RR42_RS18420 0.60 -1.2 3,990,967 + RR42_RS18420 0.64 -1.6 3,990,968 - RR42_RS18420 0.64 -0.9 3,990,991 + RR42_RS18420 0.68 -1.4 3,990,991 + RR42_RS18420 0.68 -3.5 3,990,991 + RR42_RS18420 0.68 -3.2 3,991,011 + RR42_RS18420 0.72 -3.2 3,991,011 + RR42_RS18420 0.72 -0.5 3,991,012 - RR42_RS18420 0.72 -1.5 3,991,029 + RR42_RS18420 0.75 +0.3 3,991,029 + RR42_RS18420 0.75 -3.9 3,991,031 + RR42_RS18420 0.75 -3.4 3,991,031 + RR42_RS18420 0.75 -3.4 3,991,031 + RR42_RS18420 0.75 -1.0 3,991,032 - RR42_RS18420 0.75 -0.9 3,991,032 - RR42_RS18420 0.75 -2.2 3,991,032 - RR42_RS18420 0.75 -1.3 3,991,032 - RR42_RS18420 0.75 -1.8 3,991,240 + -3.3 3,991,240 + -0.6 3,991,240 + -1.6 3,991,241 - -2.9 3,991,241 - -1.7 3,991,241 - -2.3 3,991,241 - -1.9 3,991,241 - -1.9 3,991,252 + -1.4 3,991,252 + -3.4 3,991,329 - -2.0 3,991,329 - -0.8 3,991,358 + +0.4 3,991,408 + -0.5 3,991,426 + -1.9 3,991,426 + +0.0 3,991,426 + -0.5 3,991,427 - +0.1 3,991,427 - +1.2 3,991,427 - -0.8 3,991,429 + -1.7 3,991,449 - +0.4 3,991,483 + -0.3 3,991,483 + -0.9 3,991,515 + +1.6 3,991,516 - -0.1 3,991,516 - +0.1 3,991,525 + +0.4 3,991,525 + -1.7 3,991,533 - -0.3 3,991,864 - RR42_RS18425 0.69 +0.5 3,991,902 + RR42_RS18425 0.82 +1.1 3,991,935 - +0.8 3,991,947 + +0.1 3,991,947 + +1.1 3,991,947 + -1.2 3,991,947 + -0.1 3,991,948 - -0.5 3,991,948 - +0.1 3,991,948 - -0.9 3,991,948 - +0.5 3,991,995 - -0.4 3,992,027 - +0.7 3,992,117 + -0.9 3,992,117 + -0.1 3,992,162 + -2.1 3,992,162 + +1.3 3,992,162 + +0.3 3,992,163 - +0.4 3,992,163 - -0.4 3,992,163 - +0.8 3,992,163 - +0.7 3,992,165 + +0.3 3,992,165 + +0.6 3,992,165 + -0.0 3,992,166 - +0.9 3,992,166 - +0.8 3,992,166 - -1.1 3,992,167 + +1.1 3,992,168 - -0.7 3,992,233 + -0.9 3,992,233 + -0.4 3,992,233 + -0.7 3,992,234 - +0.3 3,992,264 + -0.2 3,992,264 + -0.3 3,992,264 + +1.0 3,992,265 - +0.1 3,992,265 - +0.4 3,992,265 - -0.3 3,992,296 + -0.2 3,992,296 + -1.5 3,992,297 - +0.4 3,992,499 + +0.1 3,992,499 + +0.1 3,992,499 + -0.6 3,992,500 - +1.8 3,992,512 + -0.8 3,992,512 + -0.1 3,992,512 + -0.6 3,992,513 - +0.3 3,992,513 - -1.5 3,992,513 - -0.3 3,992,513 - -0.2 3,992,513 - +0.0 3,992,513 - +0.2 3,992,558 - +0.5 3,992,578 - -0.5 3,992,595 + +0.0 3,992,596 - -1.9 3,992,604 + -0.2 3,992,605 - -0.5 3,992,614 + -0.2 3,992,626 + +0.3 3,992,626 + -1.2 3,992,626 + +0.2 3,992,765 - RR42_RS18430 0.50 -0.2 3,992,765 - RR42_RS18430 0.50 -0.5 3,992,899 - +0.8 3,992,919 + -0.2 3,992,920 - +0.9 3,992,920 - -0.3
Or see this region's nucleotide sequence