Experiment: R2A with A22 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS18220 and RR42_RS18225 are separated by 332 nucleotides RR42_RS18225 and RR42_RS18230 are separated by 113 nucleotides RR42_RS18230 and RR42_RS18235 are separated by 295 nucleotides RR42_RS18235 and RR42_RS18240 are separated by 48 nucleotides RR42_RS18240 and RR42_RS18245 are separated by 21 nucleotides
RR42_RS18220: RR42_RS18220 - hypothetical protein, at 3,948,441 to 3,949,397
_RS18220
RR42_RS18225: RR42_RS18225 - 5S ribosomal RNA, at 3,949,730 to 3,949,842
_RS18225
RR42_RS18230: RR42_RS18230 - 23S ribosomal RNA, at 3,949,956 to 3,952,857
_RS18230
RR42_RS18235: RR42_RS18235 - tRNA-Ala, at 3,953,153 to 3,953,228
_RS18235
RR42_RS18240: RR42_RS18240 - tRNA-Ile, at 3,953,277 to 3,953,353
_RS18240
RR42_RS18245: RR42_RS18245 - 16S ribosomal RNA, at 3,953,375 to 3,954,949
_RS18245
Position (kb)
3949
3950
3951
3952
3953 Strain fitness (log2 ratio)
-1
0
1 at 3948.986 kb on + strand, within RR42_RS18220 at 3948.988 kb on + strand, within RR42_RS18220 at 3949.091 kb on + strand, within RR42_RS18220 at 3949.092 kb on - strand, within RR42_RS18220 at 3949.092 kb on - strand, within RR42_RS18220 at 3949.093 kb on - strand, within RR42_RS18220 at 3949.209 kb on + strand, within RR42_RS18220 at 3949.356 kb on + strand at 3949.356 kb on + strand at 3949.356 kb on + strand at 3949.372 kb on - strand at 3949.558 kb on + strand at 3949.559 kb on - strand at 3949.605 kb on + strand at 3949.606 kb on - strand at 3949.617 kb on + strand at 3949.630 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.01 mg/ml remove 3,948,986 + RR42_RS18220 0.57 -1.1 3,948,988 + RR42_RS18220 0.57 +0.4 3,949,091 + RR42_RS18220 0.68 -0.6 3,949,092 - RR42_RS18220 0.68 +0.0 3,949,092 - RR42_RS18220 0.68 -0.7 3,949,093 - RR42_RS18220 0.68 +0.0 3,949,209 + RR42_RS18220 0.80 +0.1 3,949,356 + -0.5 3,949,356 + +1.6 3,949,356 + -0.4 3,949,372 - +0.6 3,949,558 + -0.2 3,949,559 - -1.5 3,949,605 + +0.8 3,949,606 - +0.5 3,949,617 + -1.2 3,949,630 - +0.9
Or see this region's nucleotide sequence