Experiment: R2A with A22 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS17315 and RR42_RS17320 are separated by 164 nucleotides RR42_RS17320 and RR42_RS17325 overlap by 4 nucleotides RR42_RS17325 and RR42_RS17330 are separated by 36 nucleotides
RR42_RS17315: RR42_RS17315 - D-lactate dehydrogenase, iron-sulfur subunit GlcF (EC 1.1.99.6) (from data) , at 3,749,649 to 3,750,914
_RS17315
RR42_RS17320: RR42_RS17320 - murein hydrolase transporter LrgA, at 3,751,079 to 3,751,465
_RS17320
RR42_RS17325: RR42_RS17325 - membrane protein, at 3,751,462 to 3,752,184
_RS17325
RR42_RS17330: RR42_RS17330 - glyoxalase, at 3,752,221 to 3,752,628
_RS17330
Position (kb)
3751
3752 Strain fitness (log2 ratio)
-2
-1
0
1 at 3750.335 kb on + strand, within RR42_RS17315 at 3750.335 kb on + strand, within RR42_RS17315 at 3750.336 kb on - strand, within RR42_RS17315 at 3750.336 kb on - strand, within RR42_RS17315 at 3750.336 kb on - strand, within RR42_RS17315 at 3750.433 kb on - strand at 3750.462 kb on + strand, within RR42_RS17315 at 3750.606 kb on + strand, within RR42_RS17315 at 3750.606 kb on + strand, within RR42_RS17315 at 3750.607 kb on - strand, within RR42_RS17315 at 3750.735 kb on + strand, within RR42_RS17315 at 3750.736 kb on - strand, within RR42_RS17315 at 3750.736 kb on - strand, within RR42_RS17315 at 3750.736 kb on - strand, within RR42_RS17315 at 3750.763 kb on - strand, within RR42_RS17315 at 3750.830 kb on + strand at 3750.913 kb on - strand at 3750.913 kb on - strand at 3750.913 kb on - strand at 3751.136 kb on + strand, within RR42_RS17320 at 3751.136 kb on + strand, within RR42_RS17320 at 3751.137 kb on - strand, within RR42_RS17320 at 3751.492 kb on + strand at 3751.493 kb on - strand at 3751.527 kb on + strand at 3751.540 kb on + strand, within RR42_RS17325 at 3751.561 kb on + strand, within RR42_RS17325 at 3751.561 kb on + strand, within RR42_RS17325 at 3751.562 kb on - strand, within RR42_RS17325 at 3751.562 kb on - strand, within RR42_RS17325 at 3751.651 kb on + strand, within RR42_RS17325 at 3751.660 kb on + strand, within RR42_RS17325 at 3751.660 kb on + strand, within RR42_RS17325 at 3751.660 kb on + strand, within RR42_RS17325 at 3751.661 kb on - strand, within RR42_RS17325 at 3751.661 kb on - strand, within RR42_RS17325 at 3751.661 kb on - strand, within RR42_RS17325 at 3751.661 kb on - strand, within RR42_RS17325 at 3751.729 kb on + strand, within RR42_RS17325 at 3752.018 kb on - strand, within RR42_RS17325 at 3752.273 kb on + strand, within RR42_RS17330 at 3752.273 kb on + strand, within RR42_RS17330 at 3752.274 kb on - strand, within RR42_RS17330 at 3752.326 kb on + strand, within RR42_RS17330 at 3752.385 kb on + strand, within RR42_RS17330 at 3752.385 kb on + strand, within RR42_RS17330 at 3752.386 kb on - strand, within RR42_RS17330 at 3752.386 kb on - strand, within RR42_RS17330 at 3752.464 kb on + strand, within RR42_RS17330 at 3752.465 kb on - strand, within RR42_RS17330
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.01 mg/ml remove 3,750,335 + RR42_RS17315 0.54 -0.4 3,750,335 + RR42_RS17315 0.54 -0.6 3,750,336 - RR42_RS17315 0.54 +0.9 3,750,336 - RR42_RS17315 0.54 +1.1 3,750,336 - RR42_RS17315 0.54 +0.3 3,750,433 - +1.2 3,750,462 + RR42_RS17315 0.64 +0.2 3,750,606 + RR42_RS17315 0.76 -1.2 3,750,606 + RR42_RS17315 0.76 +0.7 3,750,607 - RR42_RS17315 0.76 -0.9 3,750,735 + RR42_RS17315 0.86 +0.0 3,750,736 - RR42_RS17315 0.86 -1.4 3,750,736 - RR42_RS17315 0.86 +1.2 3,750,736 - RR42_RS17315 0.86 +0.1 3,750,763 - RR42_RS17315 0.88 -1.3 3,750,830 + -0.1 3,750,913 - -0.4 3,750,913 - -0.8 3,750,913 - -0.2 3,751,136 + RR42_RS17320 0.15 -0.7 3,751,136 + RR42_RS17320 0.15 -0.6 3,751,137 - RR42_RS17320 0.15 -0.7 3,751,492 + +0.6 3,751,493 - +0.6 3,751,527 + +0.5 3,751,540 + RR42_RS17325 0.11 -1.8 3,751,561 + RR42_RS17325 0.14 -1.4 3,751,561 + RR42_RS17325 0.14 +0.1 3,751,562 - RR42_RS17325 0.14 +0.2 3,751,562 - RR42_RS17325 0.14 +0.2 3,751,651 + RR42_RS17325 0.26 -2.0 3,751,660 + RR42_RS17325 0.27 -0.1 3,751,660 + RR42_RS17325 0.27 +1.4 3,751,660 + RR42_RS17325 0.27 -0.6 3,751,661 - RR42_RS17325 0.28 +0.5 3,751,661 - RR42_RS17325 0.28 +0.6 3,751,661 - RR42_RS17325 0.28 +0.1 3,751,661 - RR42_RS17325 0.28 -1.5 3,751,729 + RR42_RS17325 0.37 -0.2 3,752,018 - RR42_RS17325 0.77 -1.2 3,752,273 + RR42_RS17330 0.13 -0.9 3,752,273 + RR42_RS17330 0.13 -1.2 3,752,274 - RR42_RS17330 0.13 -0.2 3,752,326 + RR42_RS17330 0.26 -0.4 3,752,385 + RR42_RS17330 0.40 -0.4 3,752,385 + RR42_RS17330 0.40 -0.3 3,752,386 - RR42_RS17330 0.40 -0.6 3,752,386 - RR42_RS17330 0.40 -0.5 3,752,464 + RR42_RS17330 0.60 -0.7 3,752,465 - RR42_RS17330 0.60 +1.2
Or see this region's nucleotide sequence