Experiment: R2A with A22 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS16530 and RR42_RS16535 are separated by 36 nucleotides RR42_RS16535 and RR42_RS16540 are separated by 264 nucleotides
RR42_RS16530: RR42_RS16530 - metallophosphoesterase, at 3,580,760 to 3,581,704
_RS16530
RR42_RS16535: RR42_RS16535 - hypothetical protein, at 3,581,741 to 3,582,088
_RS16535
RR42_RS16540: RR42_RS16540 - penicillin-binding protein, at 3,582,353 to 3,584,542
_RS16540
Position (kb)
3581
3582
3583 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3580.759 kb on + strand at 3580.759 kb on + strand at 3580.759 kb on + strand at 3580.952 kb on - strand, within RR42_RS16530 at 3581.065 kb on + strand, within RR42_RS16530 at 3581.066 kb on - strand, within RR42_RS16530 at 3581.074 kb on - strand, within RR42_RS16530 at 3581.074 kb on - strand, within RR42_RS16530 at 3581.126 kb on - strand, within RR42_RS16530 at 3581.140 kb on + strand, within RR42_RS16530 at 3581.186 kb on + strand, within RR42_RS16530 at 3581.228 kb on + strand, within RR42_RS16530 at 3581.228 kb on + strand, within RR42_RS16530 at 3581.405 kb on + strand, within RR42_RS16530 at 3581.406 kb on - strand, within RR42_RS16530 at 3581.453 kb on + strand, within RR42_RS16530 at 3581.454 kb on - strand, within RR42_RS16530 at 3581.527 kb on + strand, within RR42_RS16530 at 3581.527 kb on + strand, within RR42_RS16530 at 3581.527 kb on + strand, within RR42_RS16530 at 3581.528 kb on - strand, within RR42_RS16530 at 3581.540 kb on - strand, within RR42_RS16530 at 3581.745 kb on + strand at 3582.003 kb on - strand, within RR42_RS16535 at 3582.033 kb on - strand, within RR42_RS16535 at 3582.051 kb on + strand, within RR42_RS16535 at 3582.061 kb on + strand at 3582.232 kb on + strand at 3582.232 kb on + strand at 3582.233 kb on - strand at 3582.239 kb on - strand at 3582.242 kb on + strand at 3582.242 kb on + strand at 3582.242 kb on + strand at 3582.243 kb on - strand at 3582.298 kb on - strand at 3582.298 kb on - strand at 3582.429 kb on + strand at 3582.429 kb on + strand at 3582.521 kb on + strand at 3582.521 kb on + strand at 3582.521 kb on + strand at 3582.522 kb on - strand at 3582.522 kb on - strand at 3582.625 kb on + strand, within RR42_RS16540 at 3582.748 kb on + strand, within RR42_RS16540 at 3582.769 kb on + strand, within RR42_RS16540 at 3582.769 kb on + strand, within RR42_RS16540 at 3582.769 kb on + strand, within RR42_RS16540 at 3582.769 kb on + strand, within RR42_RS16540 at 3582.911 kb on + strand, within RR42_RS16540 at 3582.962 kb on + strand, within RR42_RS16540 at 3582.998 kb on + strand, within RR42_RS16540 at 3582.999 kb on - strand, within RR42_RS16540 at 3582.999 kb on - strand, within RR42_RS16540 at 3583.076 kb on + strand, within RR42_RS16540 at 3583.077 kb on - strand, within RR42_RS16540
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.01 mg/ml remove 3,580,759 + +0.1 3,580,759 + +0.4 3,580,759 + +0.6 3,580,952 - RR42_RS16530 0.20 -1.2 3,581,065 + RR42_RS16530 0.32 -1.6 3,581,066 - RR42_RS16530 0.32 +0.4 3,581,074 - RR42_RS16530 0.33 +0.7 3,581,074 - RR42_RS16530 0.33 -0.2 3,581,126 - RR42_RS16530 0.39 -1.2 3,581,140 + RR42_RS16530 0.40 +0.9 3,581,186 + RR42_RS16530 0.45 -0.6 3,581,228 + RR42_RS16530 0.50 +0.0 3,581,228 + RR42_RS16530 0.50 -0.3 3,581,405 + RR42_RS16530 0.68 -1.4 3,581,406 - RR42_RS16530 0.68 -0.6 3,581,453 + RR42_RS16530 0.73 -0.2 3,581,454 - RR42_RS16530 0.73 -0.4 3,581,527 + RR42_RS16530 0.81 -0.4 3,581,527 + RR42_RS16530 0.81 -0.5 3,581,527 + RR42_RS16530 0.81 +0.7 3,581,528 - RR42_RS16530 0.81 +0.2 3,581,540 - RR42_RS16530 0.83 +1.9 3,581,745 + -0.2 3,582,003 - RR42_RS16535 0.75 +0.1 3,582,033 - RR42_RS16535 0.84 +1.4 3,582,051 + RR42_RS16535 0.89 -0.9 3,582,061 + +1.4 3,582,232 + -1.1 3,582,232 + +0.0 3,582,233 - +0.8 3,582,239 - +0.1 3,582,242 + +0.3 3,582,242 + +0.2 3,582,242 + -0.8 3,582,243 - +0.4 3,582,298 - -0.4 3,582,298 - +1.2 3,582,429 + -1.6 3,582,429 + +0.4 3,582,521 + +0.6 3,582,521 + +0.2 3,582,521 + -0.4 3,582,522 - -0.6 3,582,522 - +0.1 3,582,625 + RR42_RS16540 0.12 -0.8 3,582,748 + RR42_RS16540 0.18 +0.5 3,582,769 + RR42_RS16540 0.19 -0.6 3,582,769 + RR42_RS16540 0.19 +2.2 3,582,769 + RR42_RS16540 0.19 +0.1 3,582,769 + RR42_RS16540 0.19 -0.2 3,582,911 + RR42_RS16540 0.25 +0.4 3,582,962 + RR42_RS16540 0.28 -1.8 3,582,998 + RR42_RS16540 0.29 -0.4 3,582,999 - RR42_RS16540 0.29 -0.3 3,582,999 - RR42_RS16540 0.29 +0.3 3,583,076 + RR42_RS16540 0.33 -0.4 3,583,077 - RR42_RS16540 0.33 +1.5
Or see this region's nucleotide sequence