Experiment: R2A with A22 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS16515 and RR42_RS16520 are separated by 206 nucleotides RR42_RS16520 and RR42_RS16525 overlap by 1 nucleotides RR42_RS16525 and RR42_RS16530 are separated by 126 nucleotides
RR42_RS16515: RR42_RS16515 - hypothetical protein, at 3,578,800 to 3,579,102
_RS16515
RR42_RS16520: RR42_RS16520 - RNA polymerase sigma factor, at 3,579,309 to 3,579,848
_RS16520
RR42_RS16525: RR42_RS16525 - anti-sigma factor, at 3,579,848 to 3,580,633
_RS16525
RR42_RS16530: RR42_RS16530 - metallophosphoesterase, at 3,580,760 to 3,581,704
_RS16530
Position (kb)
3579
3580
3581 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3579.349 kb on + strand at 3579.350 kb on - strand at 3579.360 kb on - strand at 3579.436 kb on - strand, within RR42_RS16520 at 3579.436 kb on - strand, within RR42_RS16520 at 3579.520 kb on - strand, within RR42_RS16520 at 3579.735 kb on + strand, within RR42_RS16520 at 3580.193 kb on + strand, within RR42_RS16525 at 3580.373 kb on + strand, within RR42_RS16525 at 3580.440 kb on - strand, within RR42_RS16525 at 3580.463 kb on + strand, within RR42_RS16525 at 3580.521 kb on - strand, within RR42_RS16525 at 3580.521 kb on - strand, within RR42_RS16525 at 3580.759 kb on + strand at 3580.759 kb on + strand at 3580.759 kb on + strand at 3580.952 kb on - strand, within RR42_RS16530 at 3581.065 kb on + strand, within RR42_RS16530 at 3581.066 kb on - strand, within RR42_RS16530 at 3581.074 kb on - strand, within RR42_RS16530 at 3581.074 kb on - strand, within RR42_RS16530 at 3581.126 kb on - strand, within RR42_RS16530 at 3581.140 kb on + strand, within RR42_RS16530 at 3581.186 kb on + strand, within RR42_RS16530 at 3581.228 kb on + strand, within RR42_RS16530 at 3581.228 kb on + strand, within RR42_RS16530 at 3581.405 kb on + strand, within RR42_RS16530 at 3581.406 kb on - strand, within RR42_RS16530 at 3581.453 kb on + strand, within RR42_RS16530 at 3581.454 kb on - strand, within RR42_RS16530 at 3581.527 kb on + strand, within RR42_RS16530 at 3581.527 kb on + strand, within RR42_RS16530 at 3581.527 kb on + strand, within RR42_RS16530 at 3581.528 kb on - strand, within RR42_RS16530 at 3581.540 kb on - strand, within RR42_RS16530
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.01 mg/ml remove 3,579,349 + +0.2 3,579,350 - -0.7 3,579,360 - -1.1 3,579,436 - RR42_RS16520 0.24 +0.1 3,579,436 - RR42_RS16520 0.24 +1.1 3,579,520 - RR42_RS16520 0.39 +0.4 3,579,735 + RR42_RS16520 0.79 +0.3 3,580,193 + RR42_RS16525 0.44 -0.5 3,580,373 + RR42_RS16525 0.67 +0.4 3,580,440 - RR42_RS16525 0.75 -2.3 3,580,463 + RR42_RS16525 0.78 -2.3 3,580,521 - RR42_RS16525 0.86 -0.4 3,580,521 - RR42_RS16525 0.86 +0.4 3,580,759 + +0.1 3,580,759 + +0.4 3,580,759 + +0.6 3,580,952 - RR42_RS16530 0.20 -1.2 3,581,065 + RR42_RS16530 0.32 -1.6 3,581,066 - RR42_RS16530 0.32 +0.4 3,581,074 - RR42_RS16530 0.33 +0.7 3,581,074 - RR42_RS16530 0.33 -0.2 3,581,126 - RR42_RS16530 0.39 -1.2 3,581,140 + RR42_RS16530 0.40 +0.9 3,581,186 + RR42_RS16530 0.45 -0.6 3,581,228 + RR42_RS16530 0.50 +0.0 3,581,228 + RR42_RS16530 0.50 -0.3 3,581,405 + RR42_RS16530 0.68 -1.4 3,581,406 - RR42_RS16530 0.68 -0.6 3,581,453 + RR42_RS16530 0.73 -0.2 3,581,454 - RR42_RS16530 0.73 -0.4 3,581,527 + RR42_RS16530 0.81 -0.4 3,581,527 + RR42_RS16530 0.81 -0.5 3,581,527 + RR42_RS16530 0.81 +0.7 3,581,528 - RR42_RS16530 0.81 +0.2 3,581,540 - RR42_RS16530 0.83 +1.9
Or see this region's nucleotide sequence