Experiment: R2A with A22 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS16150 and RR42_RS16155 are separated by 707 nucleotides RR42_RS16155 and RR42_RS16160 are separated by 84 nucleotides
RR42_RS16150: RR42_RS16150 - cell division protein ZapA, at 3,496,826 to 3,497,167
_RS16150
RR42_RS16155: RR42_RS16155 - RNA-binding protein, at 3,497,875 to 3,498,369
_RS16155
RR42_RS16160: RR42_RS16160 - membrane protein, at 3,498,454 to 3,499,266
_RS16160
Position (kb)
3497
3498
3499 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3497.126 kb on + strand, within RR42_RS16150 at 3497.191 kb on + strand at 3497.217 kb on + strand at 3497.272 kb on + strand at 3497.316 kb on + strand at 3497.316 kb on + strand at 3497.316 kb on + strand at 3497.316 kb on + strand at 3497.317 kb on - strand at 3497.317 kb on - strand at 3497.318 kb on + strand at 3497.346 kb on + strand at 3497.346 kb on + strand at 3497.346 kb on + strand at 3497.346 kb on + strand at 3497.346 kb on + strand at 3497.347 kb on - strand at 3497.347 kb on - strand at 3497.347 kb on - strand at 3497.360 kb on + strand at 3497.360 kb on + strand at 3497.360 kb on + strand at 3497.361 kb on - strand at 3497.443 kb on + strand at 3497.443 kb on + strand at 3497.546 kb on - strand at 3497.578 kb on + strand at 3497.578 kb on + strand at 3497.578 kb on + strand at 3497.578 kb on + strand at 3497.578 kb on + strand at 3497.578 kb on + strand at 3497.579 kb on - strand at 3497.611 kb on + strand at 3497.695 kb on - strand at 3497.837 kb on + strand at 3497.837 kb on + strand at 3497.837 kb on + strand at 3497.838 kb on - strand at 3498.007 kb on + strand, within RR42_RS16155 at 3498.007 kb on + strand, within RR42_RS16155 at 3498.008 kb on - strand, within RR42_RS16155 at 3498.008 kb on - strand, within RR42_RS16155 at 3498.064 kb on + strand, within RR42_RS16155 at 3498.064 kb on + strand, within RR42_RS16155 at 3498.064 kb on + strand, within RR42_RS16155 at 3498.064 kb on + strand, within RR42_RS16155 at 3498.065 kb on - strand, within RR42_RS16155 at 3498.160 kb on + strand, within RR42_RS16155 at 3498.163 kb on + strand, within RR42_RS16155 at 3498.163 kb on + strand, within RR42_RS16155 at 3498.163 kb on + strand, within RR42_RS16155 at 3498.164 kb on - strand, within RR42_RS16155 at 3498.367 kb on + strand at 3498.367 kb on + strand at 3498.367 kb on + strand at 3498.421 kb on + strand at 3498.421 kb on + strand at 3498.422 kb on - strand at 3498.425 kb on + strand at 3498.426 kb on + strand at 3498.426 kb on - strand at 3498.498 kb on + strand at 3498.606 kb on + strand, within RR42_RS16160 at 3498.633 kb on + strand, within RR42_RS16160 at 3498.800 kb on + strand, within RR42_RS16160 at 3498.800 kb on + strand, within RR42_RS16160 at 3499.045 kb on + strand, within RR42_RS16160 at 3499.046 kb on - strand, within RR42_RS16160 at 3499.046 kb on - strand, within RR42_RS16160 at 3499.046 kb on - strand, within RR42_RS16160 at 3499.096 kb on + strand, within RR42_RS16160 at 3499.102 kb on + strand, within RR42_RS16160 at 3499.102 kb on + strand, within RR42_RS16160 at 3499.103 kb on - strand, within RR42_RS16160 at 3499.103 kb on - strand, within RR42_RS16160 at 3499.103 kb on - strand, within RR42_RS16160 at 3499.103 kb on - strand, within RR42_RS16160 at 3499.103 kb on - strand, within RR42_RS16160 at 3499.103 kb on - strand, within RR42_RS16160 at 3499.234 kb on + strand at 3499.234 kb on + strand at 3499.234 kb on + strand at 3499.235 kb on - strand at 3499.304 kb on + strand at 3499.304 kb on + strand at 3499.304 kb on + strand at 3499.305 kb on - strand at 3499.305 kb on - strand at 3499.308 kb on + strand at 3499.308 kb on + strand at 3499.308 kb on + strand at 3499.309 kb on - strand at 3499.309 kb on - strand at 3499.309 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.01 mg/ml remove 3,497,126 + RR42_RS16150 0.88 +1.7 3,497,191 + -0.4 3,497,217 + -0.2 3,497,272 + -1.1 3,497,316 + +0.8 3,497,316 + -1.4 3,497,316 + -0.3 3,497,316 + -0.7 3,497,317 - +0.1 3,497,317 - -0.2 3,497,318 + +0.1 3,497,346 + -0.3 3,497,346 + -0.6 3,497,346 + -1.9 3,497,346 + -0.5 3,497,346 + -0.6 3,497,347 - -3.3 3,497,347 - -1.4 3,497,347 - -0.1 3,497,360 + -0.1 3,497,360 + -0.5 3,497,360 + -0.1 3,497,361 - -0.2 3,497,443 + -0.6 3,497,443 + -0.0 3,497,546 - +0.5 3,497,578 + +0.5 3,497,578 + -0.2 3,497,578 + -0.5 3,497,578 + -1.0 3,497,578 + +0.8 3,497,578 + +0.2 3,497,579 - -0.2 3,497,611 + -0.2 3,497,695 - -0.9 3,497,837 + -1.7 3,497,837 + +0.9 3,497,837 + +0.5 3,497,838 - -0.7 3,498,007 + RR42_RS16155 0.27 +1.3 3,498,007 + RR42_RS16155 0.27 +0.9 3,498,008 - RR42_RS16155 0.27 +0.6 3,498,008 - RR42_RS16155 0.27 -0.1 3,498,064 + RR42_RS16155 0.38 -0.0 3,498,064 + RR42_RS16155 0.38 +0.6 3,498,064 + RR42_RS16155 0.38 +1.6 3,498,064 + RR42_RS16155 0.38 +0.7 3,498,065 - RR42_RS16155 0.38 -0.5 3,498,160 + RR42_RS16155 0.58 -0.0 3,498,163 + RR42_RS16155 0.58 +1.7 3,498,163 + RR42_RS16155 0.58 +0.2 3,498,163 + RR42_RS16155 0.58 +0.5 3,498,164 - RR42_RS16155 0.58 -2.3 3,498,367 + -0.6 3,498,367 + -1.1 3,498,367 + -1.3 3,498,421 + -0.1 3,498,421 + -0.5 3,498,422 - +0.2 3,498,425 + +0.4 3,498,426 + +2.5 3,498,426 - +0.1 3,498,498 + +1.1 3,498,606 + RR42_RS16160 0.19 -1.9 3,498,633 + RR42_RS16160 0.22 +0.6 3,498,800 + RR42_RS16160 0.43 +2.6 3,498,800 + RR42_RS16160 0.43 -0.9 3,499,045 + RR42_RS16160 0.73 -0.1 3,499,046 - RR42_RS16160 0.73 +0.2 3,499,046 - RR42_RS16160 0.73 +0.1 3,499,046 - RR42_RS16160 0.73 -1.2 3,499,096 + RR42_RS16160 0.79 +1.0 3,499,102 + RR42_RS16160 0.80 +0.2 3,499,102 + RR42_RS16160 0.80 +0.6 3,499,103 - RR42_RS16160 0.80 -1.4 3,499,103 - RR42_RS16160 0.80 -0.9 3,499,103 - RR42_RS16160 0.80 -1.5 3,499,103 - RR42_RS16160 0.80 +0.2 3,499,103 - RR42_RS16160 0.80 +0.1 3,499,103 - RR42_RS16160 0.80 -1.2 3,499,234 + +0.3 3,499,234 + -0.6 3,499,234 + -0.2 3,499,235 - +0.7 3,499,304 + -0.1 3,499,304 + -0.7 3,499,304 + +0.5 3,499,305 - +1.1 3,499,305 - +0.8 3,499,308 + -0.7 3,499,308 + -0.6 3,499,308 + -0.3 3,499,309 - -0.1 3,499,309 - +1.1 3,499,309 - -0.5
Or see this region's nucleotide sequence