Experiment: R2A with A22 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS15735 and RR42_RS15740 are separated by 214 nucleotides RR42_RS15740 and RR42_RS15745 are separated by 60 nucleotides
RR42_RS15735: RR42_RS15735 - pyridoxal phosphate-dependent aminotransferase, at 3,401,123 to 3,402,277
_RS15735
RR42_RS15740: RR42_RS15740 - hypothetical protein, at 3,402,492 to 3,402,968
_RS15740
RR42_RS15745: RR42_RS15745 - group1 glycosyl transferase, at 3,403,029 to 3,404,264
_RS15745
Position (kb)
3402
3403 Strain fitness (log2 ratio)
-2
-1
0
1 at 3401.556 kb on + strand, within RR42_RS15735 at 3401.557 kb on - strand, within RR42_RS15735 at 3401.557 kb on - strand, within RR42_RS15735 at 3401.584 kb on - strand, within RR42_RS15735 at 3401.586 kb on + strand, within RR42_RS15735 at 3401.587 kb on - strand, within RR42_RS15735 at 3401.587 kb on - strand, within RR42_RS15735 at 3401.785 kb on + strand, within RR42_RS15735 at 3401.786 kb on - strand, within RR42_RS15735 at 3401.979 kb on - strand, within RR42_RS15735 at 3402.040 kb on + strand, within RR42_RS15735 at 3402.040 kb on + strand, within RR42_RS15735 at 3402.041 kb on - strand, within RR42_RS15735 at 3402.126 kb on - strand, within RR42_RS15735 at 3402.126 kb on - strand, within RR42_RS15735 at 3402.269 kb on - strand at 3402.269 kb on - strand at 3402.280 kb on + strand at 3402.281 kb on - strand at 3402.281 kb on - strand at 3402.299 kb on - strand at 3402.312 kb on - strand at 3402.478 kb on + strand at 3402.586 kb on - strand, within RR42_RS15740 at 3402.630 kb on - strand, within RR42_RS15740 at 3402.714 kb on + strand, within RR42_RS15740 at 3402.929 kb on - strand at 3403.004 kb on - strand at 3403.004 kb on - strand at 3403.011 kb on - strand at 3403.019 kb on + strand at 3403.019 kb on + strand at 3403.019 kb on + strand at 3403.019 kb on + strand at 3403.020 kb on - strand at 3403.020 kb on - strand at 3403.020 kb on - strand at 3403.020 kb on - strand at 3403.121 kb on + strand at 3403.192 kb on + strand, within RR42_RS15745 at 3403.282 kb on + strand, within RR42_RS15745 at 3403.283 kb on - strand, within RR42_RS15745 at 3403.321 kb on + strand, within RR42_RS15745 at 3403.345 kb on + strand, within RR42_RS15745 at 3403.351 kb on + strand, within RR42_RS15745 at 3403.351 kb on + strand, within RR42_RS15745 at 3403.351 kb on + strand, within RR42_RS15745 at 3403.351 kb on + strand, within RR42_RS15745 at 3403.552 kb on + strand, within RR42_RS15745 at 3403.714 kb on + strand, within RR42_RS15745 at 3403.906 kb on + strand, within RR42_RS15745 at 3403.906 kb on + strand, within RR42_RS15745 at 3403.915 kb on + strand, within RR42_RS15745
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.01 mg/ml remove 3,401,556 + RR42_RS15735 0.37 -0.2 3,401,557 - RR42_RS15735 0.38 -0.1 3,401,557 - RR42_RS15735 0.38 -0.5 3,401,584 - RR42_RS15735 0.40 -0.9 3,401,586 + RR42_RS15735 0.40 -0.6 3,401,587 - RR42_RS15735 0.40 -0.1 3,401,587 - RR42_RS15735 0.40 -1.0 3,401,785 + RR42_RS15735 0.57 -1.2 3,401,786 - RR42_RS15735 0.57 +1.3 3,401,979 - RR42_RS15735 0.74 +0.6 3,402,040 + RR42_RS15735 0.79 -0.4 3,402,040 + RR42_RS15735 0.79 -0.3 3,402,041 - RR42_RS15735 0.79 -0.4 3,402,126 - RR42_RS15735 0.87 +0.3 3,402,126 - RR42_RS15735 0.87 -0.7 3,402,269 - -0.3 3,402,269 - -0.7 3,402,280 + -0.6 3,402,281 - +1.0 3,402,281 - +0.0 3,402,299 - -0.3 3,402,312 - +1.0 3,402,478 + +0.5 3,402,586 - RR42_RS15740 0.20 -1.2 3,402,630 - RR42_RS15740 0.29 -0.6 3,402,714 + RR42_RS15740 0.47 -2.0 3,402,929 - +0.4 3,403,004 - +0.2 3,403,004 - +0.2 3,403,011 - -1.3 3,403,019 + -0.5 3,403,019 + -1.4 3,403,019 + +1.1 3,403,019 + -0.7 3,403,020 - -0.2 3,403,020 - -0.7 3,403,020 - -0.4 3,403,020 - +1.5 3,403,121 + -2.5 3,403,192 + RR42_RS15745 0.13 -1.4 3,403,282 + RR42_RS15745 0.20 -1.5 3,403,283 - RR42_RS15745 0.21 -2.2 3,403,321 + RR42_RS15745 0.24 +0.3 3,403,345 + RR42_RS15745 0.26 +0.9 3,403,351 + RR42_RS15745 0.26 -0.5 3,403,351 + RR42_RS15745 0.26 -0.7 3,403,351 + RR42_RS15745 0.26 -0.5 3,403,351 + RR42_RS15745 0.26 -0.4 3,403,552 + RR42_RS15745 0.42 +1.4 3,403,714 + RR42_RS15745 0.55 -0.7 3,403,906 + RR42_RS15745 0.71 -0.9 3,403,906 + RR42_RS15745 0.71 -0.8 3,403,915 + RR42_RS15745 0.72 -0.7
Or see this region's nucleotide sequence