Experiment: R2A with A22 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS15405 and RR42_RS15410 are separated by 282 nucleotides RR42_RS15410 and RR42_RS15415 are separated by 170 nucleotides RR42_RS15415 and RR42_RS15420 overlap by 4 nucleotides RR42_RS15420 and RR42_RS15425 are separated by 178 nucleotides
RR42_RS15405: RR42_RS15405 - peroxidase, at 3,330,933 to 3,331,571
_RS15405
RR42_RS15410: RR42_RS15410 - membrane protein, at 3,331,854 to 3,332,129
_RS15410
RR42_RS15415: RR42_RS15415 - membrane protein, at 3,332,300 to 3,332,680
_RS15415
RR42_RS15420: RR42_RS15420 - hypothetical protein, at 3,332,677 to 3,333,183
_RS15420
RR42_RS15425: RR42_RS15425 - membrane protein, at 3,333,362 to 3,334,519
_RS15425
Position (kb)
3332
3333 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3331.323 kb on - strand, within RR42_RS15405 at 3331.563 kb on + strand at 3331.563 kb on + strand at 3331.564 kb on - strand at 3331.564 kb on - strand at 3331.596 kb on - strand at 3331.596 kb on - strand at 3331.611 kb on + strand at 3331.612 kb on - strand at 3331.640 kb on + strand at 3331.642 kb on + strand at 3331.651 kb on + strand at 3331.669 kb on + strand at 3331.669 kb on + strand at 3331.672 kb on + strand at 3331.760 kb on + strand at 3331.765 kb on + strand at 3331.766 kb on - strand at 3331.768 kb on + strand at 3331.768 kb on + strand at 3331.898 kb on + strand, within RR42_RS15410 at 3332.046 kb on + strand, within RR42_RS15410 at 3332.046 kb on + strand, within RR42_RS15410 at 3332.047 kb on - strand, within RR42_RS15410 at 3332.111 kb on - strand at 3332.111 kb on - strand at 3332.127 kb on + strand at 3332.329 kb on + strand at 3332.330 kb on - strand at 3332.561 kb on + strand, within RR42_RS15415 at 3332.562 kb on - strand, within RR42_RS15415 at 3332.562 kb on - strand, within RR42_RS15415 at 3332.846 kb on - strand, within RR42_RS15420 at 3332.846 kb on - strand, within RR42_RS15420 at 3333.091 kb on + strand, within RR42_RS15420 at 3333.092 kb on - strand, within RR42_RS15420 at 3333.260 kb on - strand at 3333.288 kb on + strand at 3333.380 kb on + strand at 3333.380 kb on + strand at 3333.381 kb on - strand at 3333.549 kb on + strand, within RR42_RS15425 at 3333.620 kb on + strand, within RR42_RS15425 at 3333.621 kb on - strand, within RR42_RS15425 at 3333.659 kb on + strand, within RR42_RS15425
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.01 mg/ml remove 3,331,323 - RR42_RS15405 0.61 -1.1 3,331,563 + -0.6 3,331,563 + -0.4 3,331,564 - -0.1 3,331,564 - +1.1 3,331,596 - -0.2 3,331,596 - +0.2 3,331,611 + +0.3 3,331,612 - -0.0 3,331,640 + -0.9 3,331,642 + +1.6 3,331,651 + +0.2 3,331,669 + -1.2 3,331,669 + +0.1 3,331,672 + -2.4 3,331,760 + -0.2 3,331,765 + +0.4 3,331,766 - +1.6 3,331,768 + +1.2 3,331,768 + -0.1 3,331,898 + RR42_RS15410 0.16 +0.0 3,332,046 + RR42_RS15410 0.70 -0.1 3,332,046 + RR42_RS15410 0.70 -1.1 3,332,047 - RR42_RS15410 0.70 -0.6 3,332,111 - +0.5 3,332,111 - +0.1 3,332,127 + +0.5 3,332,329 + +0.2 3,332,330 - -1.2 3,332,561 + RR42_RS15415 0.69 +1.0 3,332,562 - RR42_RS15415 0.69 +0.3 3,332,562 - RR42_RS15415 0.69 +0.8 3,332,846 - RR42_RS15420 0.33 -0.3 3,332,846 - RR42_RS15420 0.33 +0.8 3,333,091 + RR42_RS15420 0.82 +0.7 3,333,092 - RR42_RS15420 0.82 -0.3 3,333,260 - -0.5 3,333,288 + -0.2 3,333,380 + -0.1 3,333,380 + -0.9 3,333,381 - -0.4 3,333,549 + RR42_RS15425 0.16 -0.3 3,333,620 + RR42_RS15425 0.22 -2.1 3,333,621 - RR42_RS15425 0.22 +2.1 3,333,659 + RR42_RS15425 0.26 +0.4
Or see this region's nucleotide sequence