Experiment: R2A with A22 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS15350 and RR42_RS15355 are separated by 58 nucleotides RR42_RS15355 and RR42_RS15360 are separated by 8 nucleotides
RR42_RS15350: RR42_RS15350 - BEC protein, at 3,320,509 to 3,321,753
_RS15350
RR42_RS15355: RR42_RS15355 - alpha/beta hydrolase, at 3,321,812 to 3,322,642
_RS15355
RR42_RS15360: RR42_RS15360 - peptidase M29, at 3,322,651 to 3,323,757
_RS15360
Position (kb)
3321
3322
3323 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3320.847 kb on - strand, within RR42_RS15350 at 3320.912 kb on + strand, within RR42_RS15350 at 3320.912 kb on + strand, within RR42_RS15350 at 3320.937 kb on - strand, within RR42_RS15350 at 3320.987 kb on + strand, within RR42_RS15350 at 3320.987 kb on + strand, within RR42_RS15350 at 3320.988 kb on - strand, within RR42_RS15350 at 3320.988 kb on - strand, within RR42_RS15350 at 3320.991 kb on - strand, within RR42_RS15350 at 3320.991 kb on - strand, within RR42_RS15350 at 3321.134 kb on + strand, within RR42_RS15350 at 3321.134 kb on + strand, within RR42_RS15350 at 3321.134 kb on + strand, within RR42_RS15350 at 3321.135 kb on - strand, within RR42_RS15350 at 3321.194 kb on + strand, within RR42_RS15350 at 3321.195 kb on - strand, within RR42_RS15350 at 3321.198 kb on - strand, within RR42_RS15350 at 3321.332 kb on + strand, within RR42_RS15350 at 3321.332 kb on + strand, within RR42_RS15350 at 3321.333 kb on - strand, within RR42_RS15350 at 3321.333 kb on - strand, within RR42_RS15350 at 3321.333 kb on - strand, within RR42_RS15350 at 3321.521 kb on + strand, within RR42_RS15350 at 3321.521 kb on + strand, within RR42_RS15350 at 3321.521 kb on + strand, within RR42_RS15350 at 3321.522 kb on - strand, within RR42_RS15350 at 3321.522 kb on - strand, within RR42_RS15350 at 3321.522 kb on - strand, within RR42_RS15350 at 3321.602 kb on + strand, within RR42_RS15350 at 3321.603 kb on - strand, within RR42_RS15350 at 3321.603 kb on - strand, within RR42_RS15350 at 3321.603 kb on - strand, within RR42_RS15350 at 3322.068 kb on + strand, within RR42_RS15355 at 3322.308 kb on + strand, within RR42_RS15355 at 3322.308 kb on + strand, within RR42_RS15355 at 3322.309 kb on - strand, within RR42_RS15355 at 3322.347 kb on - strand, within RR42_RS15355 at 3322.347 kb on - strand, within RR42_RS15355 at 3322.941 kb on - strand, within RR42_RS15360 at 3322.982 kb on + strand, within RR42_RS15360 at 3322.982 kb on + strand, within RR42_RS15360 at 3322.983 kb on - strand, within RR42_RS15360 at 3323.033 kb on + strand, within RR42_RS15360 at 3323.033 kb on + strand, within RR42_RS15360 at 3323.033 kb on + strand, within RR42_RS15360 at 3323.033 kb on + strand, within RR42_RS15360 at 3323.033 kb on + strand, within RR42_RS15360 at 3323.033 kb on + strand, within RR42_RS15360 at 3323.033 kb on + strand, within RR42_RS15360 at 3323.033 kb on + strand, within RR42_RS15360 at 3323.033 kb on + strand, within RR42_RS15360 at 3323.034 kb on - strand, within RR42_RS15360 at 3323.034 kb on - strand, within RR42_RS15360 at 3323.034 kb on - strand, within RR42_RS15360 at 3323.073 kb on - strand, within RR42_RS15360
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.01 mg/ml remove 3,320,847 - RR42_RS15350 0.27 +1.1 3,320,912 + RR42_RS15350 0.32 -0.1 3,320,912 + RR42_RS15350 0.32 -0.5 3,320,937 - RR42_RS15350 0.34 -0.3 3,320,987 + RR42_RS15350 0.38 -2.1 3,320,987 + RR42_RS15350 0.38 -0.5 3,320,988 - RR42_RS15350 0.38 -1.5 3,320,988 - RR42_RS15350 0.38 +2.7 3,320,991 - RR42_RS15350 0.39 -0.4 3,320,991 - RR42_RS15350 0.39 +0.0 3,321,134 + RR42_RS15350 0.50 -0.1 3,321,134 + RR42_RS15350 0.50 +0.2 3,321,134 + RR42_RS15350 0.50 +1.8 3,321,135 - RR42_RS15350 0.50 +0.0 3,321,194 + RR42_RS15350 0.55 +0.3 3,321,195 - RR42_RS15350 0.55 -0.3 3,321,198 - RR42_RS15350 0.55 -0.8 3,321,332 + RR42_RS15350 0.66 -0.4 3,321,332 + RR42_RS15350 0.66 +0.4 3,321,333 - RR42_RS15350 0.66 -0.3 3,321,333 - RR42_RS15350 0.66 -0.9 3,321,333 - RR42_RS15350 0.66 +1.4 3,321,521 + RR42_RS15350 0.81 -0.4 3,321,521 + RR42_RS15350 0.81 -1.5 3,321,521 + RR42_RS15350 0.81 -0.2 3,321,522 - RR42_RS15350 0.81 -1.2 3,321,522 - RR42_RS15350 0.81 +0.7 3,321,522 - RR42_RS15350 0.81 +0.1 3,321,602 + RR42_RS15350 0.88 -0.0 3,321,603 - RR42_RS15350 0.88 +0.2 3,321,603 - RR42_RS15350 0.88 +0.2 3,321,603 - RR42_RS15350 0.88 -0.2 3,322,068 + RR42_RS15355 0.31 +0.4 3,322,308 + RR42_RS15355 0.60 -1.2 3,322,308 + RR42_RS15355 0.60 +1.1 3,322,309 - RR42_RS15355 0.60 +1.1 3,322,347 - RR42_RS15355 0.64 +1.8 3,322,347 - RR42_RS15355 0.64 -0.1 3,322,941 - RR42_RS15360 0.26 +1.0 3,322,982 + RR42_RS15360 0.30 -2.4 3,322,982 + RR42_RS15360 0.30 -0.1 3,322,983 - RR42_RS15360 0.30 +0.2 3,323,033 + RR42_RS15360 0.35 +0.4 3,323,033 + RR42_RS15360 0.35 +0.6 3,323,033 + RR42_RS15360 0.35 -0.7 3,323,033 + RR42_RS15360 0.35 -1.7 3,323,033 + RR42_RS15360 0.35 +1.0 3,323,033 + RR42_RS15360 0.35 +0.4 3,323,033 + RR42_RS15360 0.35 -0.0 3,323,033 + RR42_RS15360 0.35 +1.8 3,323,033 + RR42_RS15360 0.35 -0.2 3,323,034 - RR42_RS15360 0.35 +0.7 3,323,034 - RR42_RS15360 0.35 -0.6 3,323,034 - RR42_RS15360 0.35 +0.5 3,323,073 - RR42_RS15360 0.38 +0.3
Or see this region's nucleotide sequence