Experiment: R2A with A22 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS14950 and RR42_RS14955 are separated by 326 nucleotides RR42_RS14955 and RR42_RS14960 are separated by 168 nucleotides RR42_RS14960 and RR42_RS14965 are separated by 27 nucleotides RR42_RS14965 and RR42_RS14975 are separated by 486 nucleotides
RR42_RS14950: RR42_RS14950 - membrane protein, at 3,233,333 to 3,233,800
_RS14950
RR42_RS14955: RR42_RS14955 - hypothetical protein, at 3,234,127 to 3,234,489
_RS14955
RR42_RS14960: RR42_RS14960 - tRNA-Met, at 3,234,658 to 3,234,736
_RS14960
RR42_RS14965: RR42_RS14965 - hypothetical protein, at 3,234,764 to 3,237,040
_RS14965
RR42_RS14975: RR42_RS14975 - DNA primase, at 3,237,527 to 3,239,341
_RS14975
Position (kb)
3234
3235
3236
3237
3238 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3233.796 kb on + strand at 3233.796 kb on + strand at 3233.796 kb on + strand at 3233.797 kb on - strand at 3233.938 kb on - strand at 3233.980 kb on + strand at 3233.980 kb on + strand at 3233.980 kb on + strand at 3233.980 kb on + strand at 3233.980 kb on + strand at 3233.980 kb on + strand at 3233.981 kb on - strand at 3233.981 kb on - strand at 3233.981 kb on - strand at 3233.981 kb on - strand at 3234.097 kb on + strand at 3234.098 kb on - strand at 3234.098 kb on - strand at 3234.139 kb on - strand at 3234.218 kb on - strand, within RR42_RS14955 at 3234.228 kb on + strand, within RR42_RS14955 at 3234.229 kb on - strand, within RR42_RS14955 at 3234.247 kb on + strand, within RR42_RS14955 at 3234.248 kb on - strand, within RR42_RS14955 at 3234.248 kb on - strand, within RR42_RS14955 at 3234.276 kb on + strand, within RR42_RS14955 at 3234.276 kb on + strand, within RR42_RS14955 at 3234.286 kb on + strand, within RR42_RS14955 at 3234.286 kb on + strand, within RR42_RS14955 at 3234.286 kb on + strand, within RR42_RS14955 at 3234.326 kb on - strand, within RR42_RS14955 at 3234.368 kb on - strand, within RR42_RS14955 at 3234.368 kb on - strand, within RR42_RS14955 at 3234.424 kb on - strand, within RR42_RS14955 at 3234.488 kb on - strand at 3234.556 kb on + strand at 3234.557 kb on - strand at 3234.558 kb on + strand at 3234.559 kb on - strand at 3234.789 kb on - strand at 3234.789 kb on - strand at 3234.789 kb on - strand at 3234.840 kb on - strand at 3235.256 kb on - strand, within RR42_RS14965 at 3235.613 kb on - strand, within RR42_RS14965 at 3235.613 kb on - strand, within RR42_RS14965 at 3235.613 kb on - strand, within RR42_RS14965 at 3235.632 kb on + strand, within RR42_RS14965 at 3235.876 kb on - strand, within RR42_RS14965 at 3236.472 kb on + strand, within RR42_RS14965 at 3236.473 kb on - strand, within RR42_RS14965 at 3237.023 kb on - strand at 3237.026 kb on - strand at 3237.026 kb on - strand at 3237.026 kb on - strand at 3237.071 kb on - strand at 3237.071 kb on - strand at 3237.071 kb on - strand at 3237.071 kb on - strand at 3237.377 kb on + strand at 3237.377 kb on + strand at 3237.377 kb on + strand at 3237.378 kb on - strand at 3237.378 kb on - strand at 3237.383 kb on + strand at 3237.383 kb on + strand at 3237.384 kb on - strand at 3237.384 kb on - strand at 3237.437 kb on + strand at 3237.437 kb on + strand at 3237.469 kb on + strand at 3237.469 kb on + strand at 3237.470 kb on - strand at 3237.470 kb on - strand at 3237.470 kb on - strand at 3237.497 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.01 mg/ml remove 3,233,796 + -1.6 3,233,796 + -0.7 3,233,796 + -2.0 3,233,797 - +1.2 3,233,938 - -1.8 3,233,980 + +0.4 3,233,980 + +0.5 3,233,980 + +0.1 3,233,980 + -0.7 3,233,980 + -0.4 3,233,980 + -1.3 3,233,981 - -0.5 3,233,981 - +0.3 3,233,981 - -0.8 3,233,981 - -0.1 3,234,097 + -1.8 3,234,098 - -1.0 3,234,098 - -0.6 3,234,139 - -0.9 3,234,218 - RR42_RS14955 0.25 -0.1 3,234,228 + RR42_RS14955 0.28 +1.2 3,234,229 - RR42_RS14955 0.28 -1.3 3,234,247 + RR42_RS14955 0.33 +2.3 3,234,248 - RR42_RS14955 0.33 -0.8 3,234,248 - RR42_RS14955 0.33 -0.8 3,234,276 + RR42_RS14955 0.41 -0.8 3,234,276 + RR42_RS14955 0.41 +0.9 3,234,286 + RR42_RS14955 0.44 -0.5 3,234,286 + RR42_RS14955 0.44 +0.1 3,234,286 + RR42_RS14955 0.44 -0.6 3,234,326 - RR42_RS14955 0.55 -1.1 3,234,368 - RR42_RS14955 0.66 +0.2 3,234,368 - RR42_RS14955 0.66 -0.3 3,234,424 - RR42_RS14955 0.82 -0.1 3,234,488 - +0.4 3,234,556 + +1.6 3,234,557 - +0.1 3,234,558 + +0.3 3,234,559 - +0.3 3,234,789 - +0.1 3,234,789 - -0.0 3,234,789 - -0.6 3,234,840 - -0.2 3,235,256 - RR42_RS14965 0.22 +0.0 3,235,613 - RR42_RS14965 0.37 -2.4 3,235,613 - RR42_RS14965 0.37 -0.4 3,235,613 - RR42_RS14965 0.37 -0.8 3,235,632 + RR42_RS14965 0.38 -1.6 3,235,876 - RR42_RS14965 0.49 +0.7 3,236,472 + RR42_RS14965 0.75 -1.0 3,236,473 - RR42_RS14965 0.75 -1.0 3,237,023 - -0.6 3,237,026 - -0.0 3,237,026 - -0.4 3,237,026 - -0.3 3,237,071 - +0.1 3,237,071 - +0.3 3,237,071 - +0.7 3,237,071 - -0.4 3,237,377 + -0.7 3,237,377 + +0.0 3,237,377 + -1.2 3,237,378 - -0.0 3,237,378 - -1.2 3,237,383 + +1.3 3,237,383 + -0.8 3,237,384 - +0.8 3,237,384 - -1.0 3,237,437 + -1.6 3,237,437 + -0.9 3,237,469 + -0.4 3,237,469 + -1.3 3,237,470 - +0.1 3,237,470 - -1.0 3,237,470 - +1.7 3,237,497 + -0.8
Or see this region's nucleotide sequence