Experiment: R2A with A22 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS14750 and RR42_RS14755 are separated by 48 nucleotides RR42_RS14755 and RR42_RS14760 are separated by 110 nucleotides RR42_RS14760 and RR42_RS14765 are separated by 19 nucleotides RR42_RS14765 and RR42_RS14770 are separated by 67 nucleotides
RR42_RS14750: RR42_RS14750 - peptidase PMbA, at 3,178,963 to 3,180,333
_RS14750
RR42_RS14755: RR42_RS14755 - hypothetical protein, at 3,180,382 to 3,181,008
_RS14755
RR42_RS14760: RR42_RS14760 - molybdenum cofactor biosynthesis protein MogA, at 3,181,119 to 3,181,709
_RS14760
RR42_RS14765: RR42_RS14765 - transporter, at 3,181,729 to 3,182,610
_RS14765
RR42_RS14770: RR42_RS14770 - oligoribonuclease, at 3,182,678 to 3,183,328
_RS14770
Position (kb)
3181
3182 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3180.137 kb on - strand, within RR42_RS14750 at 3180.137 kb on - strand, within RR42_RS14750 at 3180.295 kb on - strand at 3180.296 kb on + strand at 3180.296 kb on + strand at 3180.296 kb on + strand at 3180.296 kb on + strand at 3180.297 kb on - strand at 3180.297 kb on - strand at 3180.297 kb on - strand at 3180.370 kb on + strand at 3180.370 kb on + strand at 3180.371 kb on - strand at 3180.371 kb on - strand at 3180.378 kb on + strand at 3180.508 kb on - strand, within RR42_RS14755 at 3180.655 kb on + strand, within RR42_RS14755 at 3180.655 kb on + strand, within RR42_RS14755 at 3180.655 kb on + strand, within RR42_RS14755 at 3180.655 kb on + strand, within RR42_RS14755 at 3180.656 kb on - strand, within RR42_RS14755 at 3180.934 kb on - strand, within RR42_RS14755 at 3181.123 kb on + strand at 3181.124 kb on - strand at 3181.164 kb on + strand at 3181.165 kb on - strand at 3181.165 kb on - strand at 3181.165 kb on - strand at 3181.214 kb on + strand, within RR42_RS14760 at 3181.215 kb on - strand, within RR42_RS14760 at 3181.609 kb on - strand, within RR42_RS14760 at 3181.609 kb on - strand, within RR42_RS14760 at 3181.635 kb on + strand, within RR42_RS14760 at 3181.635 kb on + strand, within RR42_RS14760 at 3181.745 kb on + strand at 3181.746 kb on - strand at 3181.746 kb on - strand at 3181.746 kb on - strand at 3182.547 kb on - strand at 3182.625 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.01 mg/ml remove 3,180,137 - RR42_RS14750 0.86 +0.6 3,180,137 - RR42_RS14750 0.86 +0.7 3,180,295 - -0.5 3,180,296 + -0.3 3,180,296 + +0.0 3,180,296 + -0.4 3,180,296 + +1.4 3,180,297 - +0.5 3,180,297 - -0.8 3,180,297 - +1.0 3,180,370 + -0.3 3,180,370 + -0.5 3,180,371 - +0.9 3,180,371 - +0.1 3,180,378 + -3.3 3,180,508 - RR42_RS14755 0.20 -2.4 3,180,655 + RR42_RS14755 0.44 -0.3 3,180,655 + RR42_RS14755 0.44 +0.3 3,180,655 + RR42_RS14755 0.44 -1.0 3,180,655 + RR42_RS14755 0.44 -0.4 3,180,656 - RR42_RS14755 0.44 -0.1 3,180,934 - RR42_RS14755 0.88 -1.1 3,181,123 + -1.0 3,181,124 - -1.0 3,181,164 + +0.2 3,181,165 - -0.1 3,181,165 - -1.0 3,181,165 - -0.1 3,181,214 + RR42_RS14760 0.16 +0.2 3,181,215 - RR42_RS14760 0.16 +0.8 3,181,609 - RR42_RS14760 0.83 -0.1 3,181,609 - RR42_RS14760 0.83 +0.8 3,181,635 + RR42_RS14760 0.87 -1.5 3,181,635 + RR42_RS14760 0.87 +1.0 3,181,745 + -0.2 3,181,746 - -0.0 3,181,746 - +0.7 3,181,746 - -0.1 3,182,547 - +0.1 3,182,625 + -0.1
Or see this region's nucleotide sequence