Experiment: R2A with A22 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS13905 and RR42_RS13910 are separated by 96 nucleotides RR42_RS13910 and RR42_RS13915 are separated by 21 nucleotides
RR42_RS13905: RR42_RS13905 - fumarate hydratase, at 2,997,194 to 2,998,717
_RS13905
RR42_RS13910: RR42_RS13910 - glutamate racemase, at 2,998,814 to 2,999,626
_RS13910
RR42_RS13915: RR42_RS13915 - ATPase, at 2,999,648 to 3,001,948
_RS13915
Position (kb)
2998
2999
3000 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2997.896 kb on + strand, within RR42_RS13905 at 2997.896 kb on + strand, within RR42_RS13905 at 2997.897 kb on - strand, within RR42_RS13905 at 2998.313 kb on + strand, within RR42_RS13905 at 2998.313 kb on + strand, within RR42_RS13905 at 2998.314 kb on - strand, within RR42_RS13905 at 2998.317 kb on - strand, within RR42_RS13905 at 2998.317 kb on - strand, within RR42_RS13905 at 2998.379 kb on - strand, within RR42_RS13905 at 2998.497 kb on - strand, within RR42_RS13905 at 2998.524 kb on - strand, within RR42_RS13905 at 2998.910 kb on + strand, within RR42_RS13910 at 2998.910 kb on + strand, within RR42_RS13910 at 2998.911 kb on - strand, within RR42_RS13910 at 2999.312 kb on + strand, within RR42_RS13910 at 2999.352 kb on + strand, within RR42_RS13910 at 2999.352 kb on + strand, within RR42_RS13910 at 2999.353 kb on - strand, within RR42_RS13910 at 2999.353 kb on - strand, within RR42_RS13910 at 2999.353 kb on - strand, within RR42_RS13910 at 2999.353 kb on - strand, within RR42_RS13910 at 2999.516 kb on + strand, within RR42_RS13910 at 2999.517 kb on - strand, within RR42_RS13910 at 2999.777 kb on - strand at 2999.978 kb on + strand, within RR42_RS13915 at 2999.978 kb on + strand, within RR42_RS13915 at 2999.978 kb on + strand, within RR42_RS13915 at 3000.039 kb on + strand, within RR42_RS13915 at 3000.039 kb on + strand, within RR42_RS13915 at 3000.039 kb on + strand, within RR42_RS13915 at 3000.040 kb on - strand, within RR42_RS13915 at 3000.040 kb on - strand, within RR42_RS13915 at 3000.040 kb on - strand, within RR42_RS13915 at 3000.040 kb on - strand, within RR42_RS13915 at 3000.040 kb on - strand, within RR42_RS13915 at 3000.162 kb on + strand, within RR42_RS13915 at 3000.163 kb on - strand, within RR42_RS13915 at 3000.265 kb on - strand, within RR42_RS13915 at 3000.265 kb on - strand, within RR42_RS13915 at 3000.265 kb on - strand, within RR42_RS13915 at 3000.265 kb on - strand, within RR42_RS13915 at 3000.265 kb on - strand, within RR42_RS13915 at 3000.265 kb on - strand, within RR42_RS13915 at 3000.345 kb on + strand, within RR42_RS13915 at 3000.345 kb on + strand, within RR42_RS13915 at 3000.346 kb on - strand, within RR42_RS13915 at 3000.358 kb on - strand, within RR42_RS13915 at 3000.434 kb on + strand, within RR42_RS13915 at 3000.544 kb on + strand, within RR42_RS13915 at 3000.544 kb on + strand, within RR42_RS13915 at 3000.545 kb on - strand, within RR42_RS13915 at 3000.545 kb on - strand, within RR42_RS13915
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.01 mg/ml remove 2,997,896 + RR42_RS13905 0.46 -0.5 2,997,896 + RR42_RS13905 0.46 +0.7 2,997,897 - RR42_RS13905 0.46 -2.4 2,998,313 + RR42_RS13905 0.73 -1.1 2,998,313 + RR42_RS13905 0.73 +1.4 2,998,314 - RR42_RS13905 0.73 -2.7 2,998,317 - RR42_RS13905 0.74 -1.1 2,998,317 - RR42_RS13905 0.74 -2.1 2,998,379 - RR42_RS13905 0.78 -1.0 2,998,497 - RR42_RS13905 0.85 -2.3 2,998,524 - RR42_RS13905 0.87 -0.7 2,998,910 + RR42_RS13910 0.12 -3.6 2,998,910 + RR42_RS13910 0.12 -4.5 2,998,911 - RR42_RS13910 0.12 -1.5 2,999,312 + RR42_RS13910 0.61 -2.7 2,999,352 + RR42_RS13910 0.66 -3.9 2,999,352 + RR42_RS13910 0.66 -2.8 2,999,353 - RR42_RS13910 0.66 -1.5 2,999,353 - RR42_RS13910 0.66 -1.6 2,999,353 - RR42_RS13910 0.66 -2.7 2,999,353 - RR42_RS13910 0.66 -1.1 2,999,516 + RR42_RS13910 0.86 -2.2 2,999,517 - RR42_RS13910 0.86 -2.7 2,999,777 - -0.2 2,999,978 + RR42_RS13915 0.14 -0.2 2,999,978 + RR42_RS13915 0.14 +0.0 2,999,978 + RR42_RS13915 0.14 -0.4 3,000,039 + RR42_RS13915 0.17 -1.5 3,000,039 + RR42_RS13915 0.17 +0.3 3,000,039 + RR42_RS13915 0.17 +0.0 3,000,040 - RR42_RS13915 0.17 +0.5 3,000,040 - RR42_RS13915 0.17 +0.1 3,000,040 - RR42_RS13915 0.17 +0.0 3,000,040 - RR42_RS13915 0.17 +0.3 3,000,040 - RR42_RS13915 0.17 -0.4 3,000,162 + RR42_RS13915 0.22 +0.0 3,000,163 - RR42_RS13915 0.22 -0.4 3,000,265 - RR42_RS13915 0.27 +0.0 3,000,265 - RR42_RS13915 0.27 -0.1 3,000,265 - RR42_RS13915 0.27 -1.4 3,000,265 - RR42_RS13915 0.27 -0.4 3,000,265 - RR42_RS13915 0.27 +1.7 3,000,265 - RR42_RS13915 0.27 +1.5 3,000,345 + RR42_RS13915 0.30 -1.3 3,000,345 + RR42_RS13915 0.30 -0.1 3,000,346 - RR42_RS13915 0.30 +0.3 3,000,358 - RR42_RS13915 0.31 +0.9 3,000,434 + RR42_RS13915 0.34 -0.7 3,000,544 + RR42_RS13915 0.39 +0.2 3,000,544 + RR42_RS13915 0.39 +1.0 3,000,545 - RR42_RS13915 0.39 +0.6 3,000,545 - RR42_RS13915 0.39 +0.3
Or see this region's nucleotide sequence