Experiment: R2A with A22 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS13900 and RR42_RS13905 are separated by 285 nucleotides RR42_RS13905 and RR42_RS13910 are separated by 96 nucleotides RR42_RS13910 and RR42_RS13915 are separated by 21 nucleotides
RR42_RS13900: RR42_RS13900 - hypothetical protein, at 2,996,111 to 2,996,908
_RS13900
RR42_RS13905: RR42_RS13905 - fumarate hydratase, at 2,997,194 to 2,998,717
_RS13905
RR42_RS13910: RR42_RS13910 - glutamate racemase, at 2,998,814 to 2,999,626
_RS13910
RR42_RS13915: RR42_RS13915 - ATPase, at 2,999,648 to 3,001,948
_RS13915
Position (kb)
2997
2998
2999 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2996.380 kb on - strand, within RR42_RS13900 at 2996.472 kb on + strand, within RR42_RS13900 at 2996.473 kb on - strand, within RR42_RS13900 at 2996.473 kb on - strand, within RR42_RS13900 at 2996.498 kb on - strand, within RR42_RS13900 at 2996.506 kb on + strand, within RR42_RS13900 at 2996.923 kb on + strand at 2996.942 kb on + strand at 2996.943 kb on - strand at 2996.943 kb on - strand at 2997.018 kb on + strand at 2997.018 kb on + strand at 2997.019 kb on - strand at 2997.019 kb on - strand at 2997.019 kb on - strand at 2997.019 kb on - strand at 2997.143 kb on + strand at 2997.143 kb on + strand at 2997.143 kb on + strand at 2997.143 kb on + strand at 2997.144 kb on - strand at 2997.149 kb on + strand at 2997.248 kb on + strand at 2997.248 kb on + strand at 2997.248 kb on + strand at 2997.248 kb on + strand at 2997.248 kb on + strand at 2997.248 kb on + strand at 2997.248 kb on + strand at 2997.249 kb on - strand at 2997.249 kb on - strand at 2997.257 kb on + strand at 2997.258 kb on - strand at 2997.258 kb on - strand at 2997.261 kb on - strand at 2997.275 kb on + strand at 2997.275 kb on + strand at 2997.896 kb on + strand, within RR42_RS13905 at 2997.896 kb on + strand, within RR42_RS13905 at 2997.897 kb on - strand, within RR42_RS13905 at 2998.313 kb on + strand, within RR42_RS13905 at 2998.313 kb on + strand, within RR42_RS13905 at 2998.314 kb on - strand, within RR42_RS13905 at 2998.317 kb on - strand, within RR42_RS13905 at 2998.317 kb on - strand, within RR42_RS13905 at 2998.379 kb on - strand, within RR42_RS13905 at 2998.497 kb on - strand, within RR42_RS13905 at 2998.524 kb on - strand, within RR42_RS13905 at 2998.910 kb on + strand, within RR42_RS13910 at 2998.910 kb on + strand, within RR42_RS13910 at 2998.911 kb on - strand, within RR42_RS13910 at 2999.312 kb on + strand, within RR42_RS13910 at 2999.352 kb on + strand, within RR42_RS13910 at 2999.352 kb on + strand, within RR42_RS13910 at 2999.353 kb on - strand, within RR42_RS13910 at 2999.353 kb on - strand, within RR42_RS13910 at 2999.353 kb on - strand, within RR42_RS13910 at 2999.353 kb on - strand, within RR42_RS13910 at 2999.516 kb on + strand, within RR42_RS13910 at 2999.517 kb on - strand, within RR42_RS13910
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.01 mg/ml remove 2,996,380 - RR42_RS13900 0.34 -1.0 2,996,472 + RR42_RS13900 0.45 -0.2 2,996,473 - RR42_RS13900 0.45 +1.3 2,996,473 - RR42_RS13900 0.45 -0.4 2,996,498 - RR42_RS13900 0.48 -0.3 2,996,506 + RR42_RS13900 0.49 +0.3 2,996,923 + +0.4 2,996,942 + +0.2 2,996,943 - +0.6 2,996,943 - +0.0 2,997,018 + -0.1 2,997,018 + -0.0 2,997,019 - -0.1 2,997,019 - -0.4 2,997,019 - +0.6 2,997,019 - +0.7 2,997,143 + +0.3 2,997,143 + -0.0 2,997,143 + +1.1 2,997,143 + +0.3 2,997,144 - -0.4 2,997,149 + +0.6 2,997,248 + -0.5 2,997,248 + +0.4 2,997,248 + +0.7 2,997,248 + -0.2 2,997,248 + -0.5 2,997,248 + -0.3 2,997,248 + -1.0 2,997,249 - -2.4 2,997,249 - -3.8 2,997,257 + +0.4 2,997,258 - -3.1 2,997,258 - -0.6 2,997,261 - -2.4 2,997,275 + +0.7 2,997,275 + +0.4 2,997,896 + RR42_RS13905 0.46 -0.5 2,997,896 + RR42_RS13905 0.46 +0.7 2,997,897 - RR42_RS13905 0.46 -2.4 2,998,313 + RR42_RS13905 0.73 -1.1 2,998,313 + RR42_RS13905 0.73 +1.4 2,998,314 - RR42_RS13905 0.73 -2.7 2,998,317 - RR42_RS13905 0.74 -1.1 2,998,317 - RR42_RS13905 0.74 -2.1 2,998,379 - RR42_RS13905 0.78 -1.0 2,998,497 - RR42_RS13905 0.85 -2.3 2,998,524 - RR42_RS13905 0.87 -0.7 2,998,910 + RR42_RS13910 0.12 -3.6 2,998,910 + RR42_RS13910 0.12 -4.5 2,998,911 - RR42_RS13910 0.12 -1.5 2,999,312 + RR42_RS13910 0.61 -2.7 2,999,352 + RR42_RS13910 0.66 -3.9 2,999,352 + RR42_RS13910 0.66 -2.8 2,999,353 - RR42_RS13910 0.66 -1.5 2,999,353 - RR42_RS13910 0.66 -1.6 2,999,353 - RR42_RS13910 0.66 -2.7 2,999,353 - RR42_RS13910 0.66 -1.1 2,999,516 + RR42_RS13910 0.86 -2.2 2,999,517 - RR42_RS13910 0.86 -2.7
Or see this region's nucleotide sequence