Experiment: R2A with A22 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS13300 and RR42_RS13305 are separated by 114 nucleotides RR42_RS13305 and RR42_RS13310 are separated by 531 nucleotides
RR42_RS13300: RR42_RS13300 - recombinase RecB, at 2,868,325 to 2,871,240
_RS13300
RR42_RS13305: RR42_RS13305 - hypothetical protein, at 2,871,355 to 2,871,621
_RS13305
RR42_RS13310: RR42_RS13310 - thioredoxin, at 2,872,153 to 2,872,479
_RS13310
Position (kb)
2871
2872 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2871.486 kb on - strand, within RR42_RS13305 at 2871.629 kb on - strand at 2871.687 kb on - strand at 2871.777 kb on + strand at 2871.781 kb on + strand at 2871.781 kb on + strand at 2871.784 kb on - strand at 2871.835 kb on + strand at 2871.836 kb on - strand at 2871.857 kb on + strand at 2871.900 kb on + strand at 2871.936 kb on + strand at 2871.988 kb on + strand at 2871.990 kb on + strand at 2871.990 kb on + strand at 2871.993 kb on + strand at 2871.993 kb on + strand at 2871.993 kb on + strand at 2871.993 kb on + strand at 2871.993 kb on + strand at 2871.994 kb on - strand at 2871.994 kb on - strand at 2871.995 kb on + strand at 2871.995 kb on + strand at 2871.995 kb on + strand at 2871.995 kb on + strand at 2871.995 kb on + strand at 2871.995 kb on + strand at 2871.995 kb on + strand at 2871.996 kb on - strand at 2871.996 kb on - strand at 2871.996 kb on - strand at 2872.089 kb on + strand at 2872.089 kb on + strand at 2872.123 kb on + strand at 2872.477 kb on + strand at 2872.481 kb on - strand at 2872.523 kb on + strand at 2872.524 kb on - strand at 2872.576 kb on + strand at 2872.576 kb on + strand at 2872.576 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.01 mg/ml remove 2,871,486 - RR42_RS13305 0.49 -0.9 2,871,629 - -1.7 2,871,687 - -0.8 2,871,777 + +1.6 2,871,781 + +0.8 2,871,781 + +0.2 2,871,784 - -2.2 2,871,835 + -0.2 2,871,836 - -0.7 2,871,857 + -0.1 2,871,900 + -0.8 2,871,936 + -0.7 2,871,988 + -0.4 2,871,990 + +0.8 2,871,990 + -1.2 2,871,993 + +0.3 2,871,993 + -0.6 2,871,993 + +0.2 2,871,993 + -0.3 2,871,993 + -0.1 2,871,994 - +0.4 2,871,994 - +0.3 2,871,995 + -1.0 2,871,995 + +1.0 2,871,995 + -0.3 2,871,995 + -1.0 2,871,995 + +0.0 2,871,995 + -0.7 2,871,995 + -0.8 2,871,996 - -0.7 2,871,996 - +0.0 2,871,996 - -0.8 2,872,089 + +2.8 2,872,089 + +0.2 2,872,123 + -0.6 2,872,477 + -0.6 2,872,481 - -1.8 2,872,523 + -2.2 2,872,524 - +0.3 2,872,576 + +0.8 2,872,576 + -0.6 2,872,576 + -1.2
Or see this region's nucleotide sequence