Experiment: R2A with A22 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS13050 and RR42_RS13055 are separated by 58 nucleotides RR42_RS13055 and RR42_RS13060 are separated by 29 nucleotides RR42_RS13060 and RR42_RS13065 are separated by 88 nucleotides
RR42_RS13050: RR42_RS13050 - transaldolase, at 2,811,401 to 2,812,339
_RS13050
RR42_RS13055: RR42_RS13055 - NAD-dependent protein deacylase, at 2,812,398 to 2,813,144
_RS13055
RR42_RS13060: RR42_RS13060 - 23S rRNA methyltransferase, at 2,813,174 to 2,813,917
_RS13060
RR42_RS13065: RR42_RS13065 - ribonuclease R, at 2,814,006 to 2,816,597
_RS13065
Position (kb)
2813
2814 Strain fitness (log2 ratio)
-2
-1
0
1 at 2812.202 kb on - strand, within RR42_RS13050 at 2812.331 kb on - strand at 2812.889 kb on + strand, within RR42_RS13055 at 2812.940 kb on - strand, within RR42_RS13055 at 2812.940 kb on - strand, within RR42_RS13055 at 2812.940 kb on - strand, within RR42_RS13055 at 2812.973 kb on - strand, within RR42_RS13055 at 2813.090 kb on + strand at 2813.090 kb on + strand at 2813.090 kb on + strand at 2813.091 kb on - strand at 2813.091 kb on - strand at 2813.091 kb on - strand at 2813.091 kb on - strand at 2813.091 kb on - strand at 2813.091 kb on - strand at 2813.146 kb on + strand at 2813.147 kb on - strand at 2813.208 kb on + strand at 2813.209 kb on - strand at 2813.209 kb on - strand at 2813.367 kb on + strand, within RR42_RS13060 at 2813.367 kb on + strand, within RR42_RS13060 at 2813.367 kb on + strand, within RR42_RS13060 at 2813.367 kb on + strand, within RR42_RS13060 at 2813.367 kb on + strand, within RR42_RS13060 at 2813.368 kb on - strand, within RR42_RS13060 at 2813.464 kb on - strand, within RR42_RS13060 at 2813.831 kb on + strand, within RR42_RS13060 at 2813.831 kb on + strand, within RR42_RS13060 at 2813.831 kb on + strand, within RR42_RS13060 at 2813.832 kb on - strand, within RR42_RS13060 at 2813.835 kb on + strand, within RR42_RS13060 at 2813.836 kb on - strand, within RR42_RS13060 at 2813.836 kb on - strand, within RR42_RS13060 at 2813.836 kb on - strand, within RR42_RS13060 at 2813.836 kb on - strand, within RR42_RS13060 at 2813.911 kb on + strand at 2813.928 kb on + strand at 2813.928 kb on + strand at 2813.928 kb on + strand at 2813.929 kb on - strand at 2813.929 kb on - strand at 2813.929 kb on - strand at 2814.541 kb on + strand, within RR42_RS13065 at 2814.542 kb on - strand, within RR42_RS13065 at 2814.602 kb on - strand, within RR42_RS13065 at 2814.631 kb on + strand, within RR42_RS13065 at 2814.632 kb on - strand, within RR42_RS13065 at 2814.847 kb on + strand, within RR42_RS13065 at 2814.848 kb on - strand, within RR42_RS13065 at 2814.854 kb on + strand, within RR42_RS13065
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.01 mg/ml remove 2,812,202 - RR42_RS13050 0.85 +0.8 2,812,331 - -1.8 2,812,889 + RR42_RS13055 0.66 +0.0 2,812,940 - RR42_RS13055 0.73 +0.3 2,812,940 - RR42_RS13055 0.73 -0.3 2,812,940 - RR42_RS13055 0.73 -0.2 2,812,973 - RR42_RS13055 0.77 -0.4 2,813,090 + +1.1 2,813,090 + +0.9 2,813,090 + +0.2 2,813,091 - -0.0 2,813,091 - +1.0 2,813,091 - +0.2 2,813,091 - +1.0 2,813,091 - +0.6 2,813,091 - -0.3 2,813,146 + +0.5 2,813,147 - -0.3 2,813,208 + +0.8 2,813,209 - -1.3 2,813,209 - -0.2 2,813,367 + RR42_RS13060 0.26 -2.2 2,813,367 + RR42_RS13060 0.26 -2.2 2,813,367 + RR42_RS13060 0.26 -1.3 2,813,367 + RR42_RS13060 0.26 -0.5 2,813,367 + RR42_RS13060 0.26 -0.7 2,813,368 - RR42_RS13060 0.26 -0.9 2,813,464 - RR42_RS13060 0.39 -0.0 2,813,831 + RR42_RS13060 0.88 +0.4 2,813,831 + RR42_RS13060 0.88 -1.1 2,813,831 + RR42_RS13060 0.88 -1.0 2,813,832 - RR42_RS13060 0.88 -0.1 2,813,835 + RR42_RS13060 0.89 +1.0 2,813,836 - RR42_RS13060 0.89 -1.3 2,813,836 - RR42_RS13060 0.89 -0.9 2,813,836 - RR42_RS13060 0.89 +0.2 2,813,836 - RR42_RS13060 0.89 -1.8 2,813,911 + +0.3 2,813,928 + -0.1 2,813,928 + +0.3 2,813,928 + -0.3 2,813,929 - -1.6 2,813,929 - +0.1 2,813,929 - +1.4 2,814,541 + RR42_RS13065 0.21 +0.4 2,814,542 - RR42_RS13065 0.21 -0.2 2,814,602 - RR42_RS13065 0.23 -1.8 2,814,631 + RR42_RS13065 0.24 +0.5 2,814,632 - RR42_RS13065 0.24 -0.9 2,814,847 + RR42_RS13065 0.32 +1.3 2,814,848 - RR42_RS13065 0.32 +0.2 2,814,854 + RR42_RS13065 0.33 -0.6
Or see this region's nucleotide sequence